Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 1, Problem 17P
Interpretation Introduction

Interpretation:

The ratio of protonated to deprotonated form of acid is to be determined.

Concept introduction:

The ratio of protonated to de protonated form of an acid can be determined by the use of following expression,

pH=pKa+log([ A ][HA]) ...... (1)

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What are the components of a lysine buffer at pH 9.2? Refer to the following forms of lysine: A H B H H H H;N-C-COOH H;N-C-coo- H,N-C-c0- H,N-C-coo- CH2 CH2 CH2 CH. CH2 CH, pk-2.17 pk=4.04 pk=12.48 CH, CH, ÇH2 CH, CH. CH, CH, CH. CH, CH, NH, NH, NH, NH, . A and B .C and D .B and C . A and D B and D
pH of solution 14.00 12.00 10.00 8.00 6.00 First 4.00 equivalence point 2.00 0 First midpoint Second equivalence point Third midpoint pH = pKa=12.32 Third equivalence point HPO4(aq) + OH(aq) PO4(aq) + H2O(1) Second midpoint pH = pKa = 7.21 H2PO4 (aq) + OH(aq) HPO42 (aq) + H2O(1) Using the Henderson- Hasselback equation, show how to create 2L of a 0.1 M KPhos pH 7.5 buffer using K2HPO4 and KH2PO4. The chart to the left should help you understand what pKa to start with. Show your work. pH = pKa = 2.16 H3PO4(aq) + OH(aq) H2PO4(aq) + H2O(l) 25.0 50.0 75.0 100.0 Volume of NaOH added (mL)
A 0.200 M solution of a weak monoprotic acid (HA) has a pH of 2.35. What is the value of K of this acid? a K =2.011e-4 a What is the percent ionization of this acid? Percent Ionization = .10 %
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