Three grams of soil from the ground were added to 27 mL of sterile water and shaken vigorously. After the soil particles settled, 0.1 mL of this was added to 9.9 mL of sterile water. This was further diluted by 4 successive 1/10 dilutions. One mL from the last dilution was used to prepare a pour plate. After incubation, 289 colonies were present on this plate. What was the number of colony-forming units/gram of the soil?

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Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.