A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting it with 450 ml of sterile distilled water. One ml and 0.1 ml of sample suspension were inoculated in 50 ml Dextrose Tryptone Bromcresol Purple agar and were heated at 100˚C for 5 minutes. Each flask was poured into 3 plates. After the incubation, opaque colonies surrounded by a yellow halo in purple field were counted. The following counts were obtained from the plates: 95, 107, 92, 26, 28 and 27. Compute for the total spore counts and express your final answer per 50 grams of the powdered milk sample.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting it with 450 ml of sterile distilled water. One ml and 0.1 ml of sample suspension were inoculated in 50 ml Dextrose Tryptone Bromcresol Purple agar and were heated at 100˚C for 5 minutes. Each flask was poured into 3 plates. After the incubation, opaque colonies surrounded by a yellow halo in purple field were counted. The following counts were obtained from the plates: 95, 107, 92, 26, 28 and 27. Compute for the total spore counts and express your final answer per 50 grams of the powdered milk sample. 

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