Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample, added urea, and then let it incubate for one week. She then performed serial dilutio shown below:
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- Joseph, being a know-it-all, told Mary that her method would not include all microbes. To demonstrate this, he took another 10g sample of Mary's garden and added starch. After the 1- week incubation time, he performed the same serial dilution scheme as Mary but used pour plating instead of spread plating. Shown below are the counts that he obtained after the incubation period. Compute for the CFU/g given the data below. Be sure to show complete solutions and box your final answer. Final answers should be expressed in 3 significant figures. Tube 1 135, 129 Tube 2 9.7Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uLAnswer each of the questions below using the plate images provided. Nutrient Agar 1 EMB Blood Agar 1. How many colonies are on NA plate 3?. 2. How many bacteria were transferred to NA plate 3?. 3. How many colonies are on NA plate 2? 4. How many bacteria were transferred to NA plate 2?. 5. How would you describe the growth on NA plate 1?. 6. How many colonies grew together on NA plate 1? 7. How many bacteria were transferred to NA plate 1? Nutrient Agar 2 8. How many bacteria collected from the source? (Hint: What fraction of tube 1 was spread on NA plate 1?) 9. Were any of the bacteria from the source Gram-negative? How can you tell?. 10. Could any of the bacteria from the source ferment lactose? How can you tell? Nutrient Agar 3 11. Were any of the bacteria from the source hemolytic? How can you tell?. 00
- Provided with the following data, compute the corresponding CFU/ml of the original culture. ssume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22What was the concentration of bacteria (CFU/mL) in the original suspensions used for dilutions and plating below: Dilution Amount plated # of colonies counted 10-4 1.0 mL 271 10-5 0.1 mL 36 10-6 1.0 mL 9Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
- Five grams of soil were added to 45 ml of sterile water and shaken vigorously. After that, 0.1 ml of this was added to 9.9 ml of sterile water. This was further diluted by four successive 1/10 dilutions. The last dilution was used to prepare a spread plate. After incubation, 58 colonies were present on this plate. What is the CFU/g of the soil sample?A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank (tube A) and then serially diluted five (5) more times by transferring 1 ml of a previous dilution into a tube of 9 ml of sterile water. After making these serial dilutions, 2 ml samples were taken from tubes D, E, and F, added into empty petri dishes, and molten PCA was poured into each petri dish. Tubes A, B, and C were heated in a 80˚C water bath for 30 minutes, 2 ml samples of these tubes were added into empty petri dishes, and molten PCA was poured into each petri dish. These plates were allowed to solidify, and they were incubated at 35˚C for 48 hours. After the incubation period, you counted CFUs on all 6 plates. The results of your CFU counts are contained in table 1. Table 1. CFU counts for 3 plates from the heated dilutions (A-C) and the 3 plates from the non-heated dilutions (D-F) # CFUs Not heated Heated A -- 400 B -- 45 C -- 6 D 350 -- E 31 -- F 2 --…You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?
- First There Was One, Then There Were Two RESULTS Results of the plate counts for the samples you worked with. Number of Colonies for Each Dilution Plated Time Sampled 10-3 10-4 10-5 10-6 10-7 10-8 10-9 10:40 TNTC TNTC TNTC 280 TFTC TFTC3 T FIC2 TFTC TFTC 13 TNTC TNTU TNTC TNTC I60 TNTC TNTC 1:00 TFTC q a TFTC O 1:20 11:40 12:00 TNTC TNTC87 TFTC TFTC, TFICg TNTC ITNTC ITNTC TNTC 125 TNTC TNTC TNTC TNTC 164 TFIC 13 T+TC Calculate the cfu/ml for each time sample you worked with in the space below and record those values in the table below.Provided with the following data, compute the corresponding CFU/ml of the original culture. Assume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22 2Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?