in a specrophotometry ethanol determination experiment, you measured OD340 and assumed this corresponds to the rise in NADH concentration. How can you increase your confidence that you actually measured a change of NADH concentration (using a plate reader/spectrophotometer) ?
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in a specrophotometry ethanol determination experiment, you measured OD340 and assumed this corresponds to the rise in NADH concentration. How can you increase your confidence that you actually measured a change of NADH concentration (using a plate reader/spectrophotometer) ?
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- Under controlled conditions, a transpiration experiment was conducted using two different plant species, A and B. Each plant species was exposed to a low-humidity environment and a high-humidity environment with all other conditions kept the same. The transpiration rate was measured over a period of one hour. The data was graphed as shown below. A B transpiration rate (pl/cm²min) с 0.2 0.18 0.16 0.14 0.12 0.1 0.08 0.06 A bar graph of the transpiration rates of two plants under different humid conditions D 0.04 0.02 low humidity 40% In order to see the effect the change in humidity had on the transpiration rate, what would be a proper con trol to add to the study? high humidity 80% running the same experiment multiple times to collect additional data adding more water to the original plants and running the experiment again grow another set of plants at normal humidity growing more plants at 0% and 100% humidity Species A Species BUnder controlled conditions, a transpiration experiment was conducted using two different plant species, A and B. Each plant species was exposed to a low-humidity environment and a high-humidity environment with all other conditions kept the same. The transpiration rate was measured over a period of one hour. The data was graphed as shown below. A B transpiration rate (pl/cm²min) с 0.2 0.18 0.16 0.14 0.12 0.1 A bar graph of the transpiration rates of two plants under different humidity conditions What is the independent variable in the experiment? D 0.08 0.06 0.04 0.02 low humidity 40% the type of soll each plant was grown in the amount of water lost the humidity the plants are grown in high humidity 80% the amount of water given to each plant Species A Species BLab students placed ten spinach leaf disks in each of three test tubes containing a baking soda solution and a control tube without baking soda. The volume of liquid in each tube and the temperature of the solutions were held constant. All tubes were placed an equal distance from the light source indicated in the accompanying table. After 10 minutes, the amount of oxygen present in each sample was measured. Test Tube Grams of Baking Soda (CO2 source) Wattage of Light Bulb 1 0.5 25 2 0.5 50 3 0.5 75 4 0 75 Which tube is expected to produce the least amount of oxygen? a) Tube 1 b) Tube 2 c) Tube 3 d) Tube 4
- For scenario 2, below write the following 1.the process that the sets of measurements represent. 2.suitable equations of the process. 3.the calculated rates and 4.the resulting units for the process as expressed by your calculation. Scenario 2 Time,0 minutes:7.13mg/L O2 Time,20 minutes:13.39mg/L O2 Algal biomass:2.3g fresh massIn a photosynthetic investigation, a student measured the following pH of the stroma and the inner thylakoid compartments of spinach leaf cells at the conclusion of the experiment. Source of Fluid pH (mean) Standard Error of Mean (SEM) Stroma 8.2 0.75 Inner compartment of thylakoid 5.6 0.35 b. Construct an appropriately labeled graph to illustrate the sample means of the two locations to within 95% confidence (i.e. sample mean + 2 SEM).Lab students placed ten spinach leaf disks in each of three test tubes containing a baking soda solution and a control tube without baking soda. The volume of liquid in each tube and the temperature of the solutions were held constant. All tubes were placed an equal distance from a light source as indicated in the accompanying table. After 10 minutes, the amount of oxygen present in each sample was measured. Test Tube Grams of Baking Soda (CO2 source) Wattage of Light Bulb 1 0.5 25 2 0.5 50 3 0.5 75 4 0 75 Which tube is expected to contain the greatest quantity of oxygen? Tube 1 Tube 2 Tube 3 Tube 4
- A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*KWhen the yeast cells have completely re-hydrated, measure out 1.8 mL of well-mixed yeast suspension (0.2% yeast) into each of two new 15 mL centrifuge tubes. Add 200 μL of a 10% (w/v) Sodium Azide*** in YGM solution to one of the 15 mL tubes with yeast suspension. Add 200 μl of YGM to the other 15 mL tube of yeast suspension for your Control. Let both tubes incubate at room temperature for 30 min, vortexing every ~5 minutes What is the % concentration of azide in this sample during metabolic inhibition?What is the % concentration of yeast in this sample during metabolic inhibition?A 10-L stirred tank is used to cultivate mouse myeloma cells. Dry air is sparged from the bottom at 1.0 L/min to supply oxygen to the system. The gas composition at the outler is 16% oxygen, 3.5% CO2, 6.5% water vapor, and balanced nitrogen. The ambient pressure and temperature are 1 atm and 37 °C, respectively. The dissolved oxygen in the reactor is controlled at 20% of saturation with ambient air. a. Assuming the reactor is at a steady-state, what is the volumetric oxygen transfer coefficient (kLa)? b. The specific OUR of myeloma cells is 5*10-11 mmol/cell*h. What is the cell concentration? c. What is the respiratory quotinent (RQ) (i.e., mole CO2 produced/mol O2 consumed)? Assume that the mole fraction of CO2 in the ambient air is negligible. The universal gas constant R = 0.082 L*atm/mol*K
- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206Figure 1 show the shape of the peaks in gas chromatography that causes loss of column efficiency. Determine the type of shape and identify two possible solutions to fix it. INJ Time (min)An activated sludge system operates at a flow rate (Q) of 400 m³/day (and an HRT of 0.129 day) with an incoming BOD (So) of 300 mg/L. Through pilot plant work, the kinetic constants for this system are determined to be Y = 0.5 kg SS/kg BOD, Ks = 200 mg/L, and 2/day. What mixed liquor concentration is necessary to attain a 95% BOD removal? What will the corresponding MCRT be? =