Calculate the percentage efficiency of extraction of phycocyanin in sample 3, if the amount of phycocyanin is 57.4 mg, and the starting amount in sample 1 was 119.2 mg. Your answer should have one decimal place.
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Calculate the percentage efficiency of extraction of phycocyanin in sample 3, if the amount of phycocyanin is 57.4 mg, and the starting amount in sample 1 was 119.2 mg. Your answer should have one decimal place.
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- Calculate the percentage efficiency of extraction of phycocyanin in sample y, if the amount of phycocyanin is 57.8 mg, and the starting amount in sample x was 118.1 mg. Your answer should have one decimal place.Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.192 and A650=0.090, taking into account the dilution factor (100), and the total volume of extract (45). Note your answer to 2 decimal places.
- Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.211 and A650=0.086, taking into account the dilution factor (100), and the total volume of extract (140).Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.Calculate the volume of BSA stock that will be required to make the standard solutions needed to create the BSA standard curve. Be sure to show your work and include the volume of 0.02 M phosphate buffer required to reach a final volume of 1 mL. From a 2,000 μg/mL BSA stock, create 1 mL of each of the following stock solutions in 0.02 M phosphate buffer using individual microcentrifuge tubes: 50 μg/mL, 250 μg/mL, 500 μg/mL, 1,000 μg/mL, 1,250 μg/mL, 1,500 μg/mL. Be sure to properly label all the microcentrifuge tubes before creating the standards.
- You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.Pretend the results below were obtained for water from your well. The 10 mL tubes are Full Strength, or 9.9 mL of the sample plus 0.1 mL of 10x broth (here a fluorescent Brilliant Green Broth). The 1 mL tubes are 1 mL sample plus 9 mL of normal broth (so a ten-fold dilution). The 0.1 mL are that much sample with 9.9 mL of the broth (so a hundred-fold dilution). Dark green indicates coliform presence. Use the table and the figure and give me the MPN. To do so, count the number of positive tubes at each dilution. Then match the counts to the set of values that most closely fits. Yes, you need to consider the set of values for None, 1 and 0.1 taken together. For example, 5,2,1. What is the MPN estimate for this well water sample? If safe drinking water is <5 cells/100 mL would you drink this well water?
- The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?You will be given 1 mL of a 2.0 mg/mL solution of bovine serum albumin (BSA) and 1 mL of a 2.0 mg/mL solution of bovine gamma globulin (BGG). From these solutions, you will need to make 100 μL of each of the following standards using 1x MBP purification buffer as the diluent for preparation of a standard curve: 1000 μg/mL, 750 μg/mL, 500 μg/mL, 250 μg/mL, 125 μg/mL, and 0 μg/mL.What non-titrimetric method can be used to determine the iodine value of edible oil? Discuss its basic principles and general methods involved.