Interpretation:
As the isoelectric point of Histidine is 7.64, on paper electrophoresis towards which electrode does it migrate at
Concept Introduction:
The isoelectric point of an amino acid is the pH at which the molecules in solution have net charge of zero.
The paper electrophoresis is the technique which is used for the separation of amino acids. In this technique, a paper strip coated with a buffer whose pH is predetermined is placed between the two electrodes. When the sample is placed and the electrical potential is applied, the positively charged amino acids moves towards the cathode (negative electrode) and the negatively charged amino acids moves towards the anode (positive electrode). After separation, the paper strip is dried and sprayed with the dye to make the separated amino acids visible.
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Organic Chemistry
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- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning