Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 18, Problem 1TY
Summary Introduction

Introduction:

The restriction enzyme term was originated from the phage λ studies. It is a virus which infects the bacteria. It is involved in the host-controlled restriction and modification phenomenon of such bacteriophages. More than about 3,000 restriction enzymes have been known in detail. These enzymes are consistently used for the DNA modification. They act as an important tool in laboratories for the molecular cloning.

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Answer to Problem 1TY

The correct answer is the option (d). All of the above.

Explanation of Solution

Explanation/justification for the correct answer:

Option (a) are used to cleave DNA into pieces for gene cloning. Restriction enzyme is also known as restriction endonuclease. They are molecular scissors which cleaves the DNA in with intense accuracy at precise sites. They isolate the gene in the cleaved DNA fragments and is used in recombination. Thus it is extensively used in gene cloning and molecular biology techniques.

Option (b) are formed by the bacterial cells to stop the viral infection. Restriction enzyme usual role is to damage the foreign DNA that enters the cell by cleaving the DNA of bacteriophage. It prevents the cell from viral infection.

Option (c) form the sticky ends on DNA fragments. A restriction enzyme cleaves directly through the DNA strands. It then forms the DNA fragments with a blunt or flat end. This hanging nucleotide strand is known as a sticky end. It can simply bond with the complementary DNA pieces.

Option (d) all of the above. The restrictions enzyme cleaves the DNA at the precise sites and forms the sticky ends. They also stop the viral infection. Hence, the option (d) is correct.

Explanation for the incorrect answer:

Option (e) Only (a) and (c). Hence, the option (e) is incorrect.

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Students have asked these similar questions
The following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested  - DNA plasmids are chunks of chromosomal DNA used for cloning
Restriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.
Which of the following most accurately describes the process of DNA cloning?​ set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes​ ​set of laboratory procedures that uses living cells to mass-produce specific DNA fragments ​set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip ​the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology ​set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmid
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