What is the purpose of the quadrant streak plate method? - How could this method be used in a clinical microbiology laboratory? - What happens if you do not flame the loop in between quadrants? Please type
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- What is the purpose of the quadrant streak plate method?
- How could this method be used in a clinical
- What happens if you do not flame the loop in between quadrants?
Please type
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- What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle- What is a pure culture? - Describe the procedure for making a streak plate from a clinical specimen (like a urine culture). - What is the desired outcome (result) of a streak plate? - Clinically, why is it important to make a pure culture from a clinical specimen? What do you do next in order to treat the patient?What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop or needle long enough? Why is it necessary to cool the inoculating loop prior to obtaining the bacterial sample? In which direction should you move the inoculating loop in the Bunsen burner flame? (i.e. from the handle to the loop or from the loop to the handle)
- When bacteria from a throat swab are streaked on blood agar, why is the agar stabbed several times with the loop?A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusDescribe the process to make a 4-phase streak plate beginning with your first streak (i.e you have bacterial culture on your sterile loop and are about to start to streak your agar plate ).
- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedExplain why it is important to use only a small amount of bacteria when preparing a smear. What are the two things that are stained in a capsule stain? What is NOT stained in a capsule stain?Write the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates. Note: This will perform the students so please write it in the easiest manner. Thanks
- What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of an E-test strip.What is the difference between streak plate method and spread plate technique?In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.