Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth culture, removed the cap, and flamed the mouth of the tube. He inserted an inoculating loop to obtain a bacterial sample. Then, he flamed the mouth of the tube and replaced the cap. Noah opened the lid of a labeled agar plate diagonally and used the loop to streak the surface of the agar. After closing the lid, he flamed the loop in an incinerator and put it back in its container. The plate was incubated upside down for 24–48 hours. What did Noah do wrong in this transfer? a. He did not use the transfer tool correctly. b. He did not handle the culture tube correctly. c. He did not handle the agar plate correctly. d. He used the wrong tool in transfer.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter7: Basic Clinical Microbiology
Section7.4: The Gram Stain
Problem 4RQ
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Noah wanted to transfer Staphylococcus aureus from a broth to an
agar plate. He picked up the broth culture, removed the cap, and
flamed the mouth of the tube. He inserted an inoculating loop to
obtain a bacterial sample. Then, he flamed the mouth of the tube
and replaced the cap. Noah opened the lid of a labeled agar plate
diagonally and used the loop to streak the surface of the agar. After
closing the lid, he flamed the loop in an incinerator and put it back in
its container. The plate was incubated upside down for 24–48 hours.
What did Noah do wrong in this transfer?
a. He did not use the transfer tool correctly.
b. He did not handle the culture tube correctly.
c. He did not handle the agar plate correctly.
d. He used the wrong tool in transfer.

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