What are the applications of hplc?

Q: The ___________ color test is used to locate and characterize seminal stains.

A: The seminal stain has a yellowish-grey hue and resembles the shape of a counter map as seen with the…

Q: Which of the following is INCORRECTLY paired? a. SDS-PAGE : Size b. Gel filtration chromatography :…

A:

Q: Identify some advantages and limitations of using HPLC, and explain

A: When organic mixtures of compounds are nonvolatile, thermally not stable, and have high molecular…

Q: What is the importance of paper chromotography?  What is the usage of paper chromotography in the…

A: Paper chromatography is a chromatography technique that is used for separating colored pigments…

Q: HSP-02-754 ppm 180 160 140 120 100 PPM 60 40 20 H. H.

A: The question is based on the concept of spectroscopy.   we have to analyse the spectral data…

Q: Red, blue, and yellow spots were found in the chromatogram. Red spot traveled twice the distance…

A:

Q: Consider the data result in determining the presence of copper has a mean value of 50 mg and…

A: We need to find out the standard deviation ( σ ).    Standard deviation is related with the…

Q: I have 20 ml of a bacterial culture. After preparing a serial dilution as shown below and plating…

A: Given: Volume plated on the petri dish = 0.2 mL cfu counted = 45

Q: What are the dispersed phase and dispersion medium in milk?

A: Dispersed Phase: The phase or particles which are present in the form of colloidal particles is…

Q: qualitative and quantitative aspects of HPLC?

A: The testing of a substance or mixture to determine the characteristics of its chemical constituents.

Q: List five factors that affect the retention time.

A: Retention time is a measure of the time taken for a solute to pass through a chromatography column.…

Q: AICI3 a) d)

A: Carbocation always have tendency to rearrangement.

Q: Write three (3) things in your mind when you heard chromatography?

A: Given  Experiment: Chromatography Chromatography is the analytical technique used to isolate the…

Q: A student performed a chromatography experiment to determine the Rf value of an unknown amino acid.…

A: The Rf value ( retention factor) of a compound is defined as the ratio of distance travelled by the…

Q: Which the pigments present on spinach leaves correspond to each spot in the chromatogram resulting…

A: When the thin layer chromatogram of spinach leaves were read under the regular fluorescent light,…

Q: paper chromatography?

A: Chromatography is a technique that is used for the separation of a mixture. It has two important…

Q: What kind of samples can be analyzed by AFM? What are the applications of AFM?

A: Atomic Force Microscopy (AFM) is a powerful technique that can image virtually any type of surface,…

Q: Find the ksp of PbCl2  5.0 g od pbcl2 100ml of h2o

A: GIVEN DATA: given mass of  PbCl2: 5gm Molar mass of PbCl2 is 278.1gmol-1 water = 100ml

Q: How resolution in HPLC analysis can be improved?

A: HPLC i.e  high-performance liquid chromatography is an analytical method which is being used to…

Q: one of the following tests is usually carried by infra red for raw materials in British pharmacopeia…

A: There are various aspects of raw material that should be checked in order for the better quality…

Q: What is DEPT used for?

A: DEPT Distortions Enhancement by Polarization Transfer   Three types of DEPT: 1) DEPT-45 2) DEPT-90…

Q: Principles of Chromatography

A: Definition :

Q: what are advangtage and disadvantage of using an Atomic Force Microscopy and explain each

A: Atomic force microscopy is a technique by which we study the different type of surfaces like…

Q: List the desirable characteristics of HPLC detectors.

A:

Q: Many ink dyes can be separated by the technique of___________ chromatography

A: Thin Layer Chromatography is a method for separating non-volatile substances. The separation…

Q: HO and ?

A: Alcohol can be defined as an organic compound in which carbon atom is attached to hydroxyl group.

Q: A TLC plate was developed with a series of anlagesics and the unknown sample. An image of the plate…

A: Rf  value of each spot = To be determined Identity Of Unknown Solution = To be Determined

Q: . What is the difference between temporary and permanent hardness?

A: Difference between temporary and permanent hardness :

Q: Aside from TLC and paper chromatography, what are the other types of chromatography. Discuss their…

A: Answer - chromatography - Chromatography is a laboratory technique for the separation of a mixture…

Q: describe different type of degasser in HPLC system, and why degassing is important in the middle of…

A: Degasser is  very useful in HPLC .the main use of degasser in HPLC are  hellium sparging, sonication…

Q: What is done in thermogravimetric analysis?

A: Thermogravimetric analysis are of three types. They are Isothermal or static thermogravimetry…

Q: What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to…

A: TLC works on the basic principle of separation of compounds based on the interaction of the sample…

Q: An example of planar chromatography is:

A: Dear student I have given answer to your question in the image format.

Q: What is the effect of temperature on chemisorption?

A: The process that deals with the accumulation of gas-solid or liquid, or gas molecules on another…

Q: Why HPLC is an important   technique in the fields of chemistry . explain with different reasons?

A: The full form of HPLC is High Performance Liquid Chromatography.

Q: The protein concentration of a protein isolate was determined using the Bradford assay. Five…

A: A question based on tools in analytical chemistry that is to be accomplished.

Q: 4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses,…

A: Pipettes are one of the most important instruments in the analytical measurements that help us in…

Q: A student wanted to determine the color components of a black marker ink and describe the behavior…

A:

Q: Please propose a chromatography-based strategy to purify human insulin proteins that have been…

A:

Q: ) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions.…

A: The main components of clove oil are eugenol, eugenol acetate and caryophyllene. The structures of…

Q: Which is more accurate, a transfer pipet or a measuring pipet? What is theuncertainty in microliters…

A: A pipette is an apparatus which is utilized to measure out and deliver out a definite volume of…

Q: Identification of spots on the TLC plate is done by all of the following EXCEPT O Spraying with…

A: TLC is thin layer chromatography used to know the components in mixture and generally used to check…

Q: Enumerate the differences between a serological and a volumetric pipette and enumerate the uses,…

A: The main difference between the volumetric and serological pipette is that The volumetric pipette…

Q: ct-

A: The total amount of metter in the universe remains constant. This is a first law of thermodynamics…

Q: Draw a skitch for GC and HPLC instruments showing all parts

A: GC and HPLC instrumentation can be given as follows

Q: Mention the relative sizes of hard gelatin capsule with their corresponding volume and fill weight…

A: HARD GELATIN CAPSULES: Hard gelatin capsules are modern invention which created a great impact in…

Micelles and Biological Membrane

Micelles are the type of lipid molecules that organize themselves in spherical shape in aqueous solutions. The production of the micelle is a reply to the amphipathic behavior of fatty acids. It means that they consist of both hydrophobic regions and hydrophilic regions. In micelles, polar head groups (hydrophilic groups) produce an outer layer associated with water whereas nonpolar tails (hydrophobic groups) are organized in the interior.