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A: Which of the following methods cannot be used to visualize spots on a TLC plate? Group of answer…
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A: Rf value of each spot = To be determined Identity Of Unknown Solution = To be Determined
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Q: how does TLC determine if a sample is pure?
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Q: We used tab water to extract caffeine from tea instead of using distilled water. How does it affect…
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Q: You are treating the TLC plate with 5% of sulfuric acid in ethanol. Briefly describe how this works.
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Q: does a co spot on TLC plate have two Rf values?
A: co-spot in TLC
What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to visualize a TLC plate?
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- A) What are the basic principles underlying TLC technique? B) Draw a TLC plate and explain how TLC can be used to identify the presence of a specific compound in an organic mixture.Although TLC is qualitative and tells you what is present in a given sample, it can also be used to determine where a compound might be lost: What samples could you test on a single TLC plate that might give you this information?What is Ultra-Performance Liquid Chromatography (UPLC)? What are the differences and advantages of UPLC over HPLC?
- 1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.5. The following TLC plate was developed in 4:1 hexane/acetone. a) Draw what the TLC plate could look like if it were developed in 9:1 hexane/acetone. b) Draw what the TLC plate could look like if it were developed in 1:1 hexane/acetone. 4:1 hexane/acetone 9:1 hexane/acetone 1:1 hexane/acetoneA student ran a TLC plate using a 90:10 hexane:ethyl acetate developer and this was the outcome. How can they improve their next trial?
- Consider the following molecules. Which statement(s) is TRUE? CH3 CH3 но ČH3 но ČD3 i. You could differentiate between the two with a spot test. ii. You could differentiate between the two with liquid chromatography. iii. You could differentiate between the two with TLC. iv. You could differentiate between the two with a microcrystalline test. O a. i and iv only O b. ii and iii only O c. iii only O d. i only e. Neither i, ii, iii, nor iv are correct statements.An example of planar chromatography is: Column chromatography HPLC GLC TLCAside from TLC and paper chromatography, what are the other types of chromatography. Discuss their principles, applications and limitations.
- How does ammonia, dimethylglyoxime (DMG), and 8-hydroxyquinoline (8HQ) interact with your cations, leading to the evolution of colored species? Context: Paper chromatography experiment (separation of inorganic cations) with a 9:1 acetone/ HCl solventHow is TLC used to determine the purity of a sample?4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.