A student performs a TLC experiment and at the end of the experiment, sees no spots other than the initial spot. What does this mean, and what should the student do to fix the problem?
Q: What cause the spikes in the spectrophotometer reading? Explain
A: In electronic spectroscopy, generally the electron excites from an initial low energy state to a…
Q: Why would you add a piece of filter paper to your TLC developing beaker (jar)?
A:
Q: What is affinity chromatography? How does it work? What is technique? What information does it give…
A:
Q: Discuss the different separation techniques. Handpicking Threshing Winnowing
A:
Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 14.9 cm and the spot…
A: Given values, Distance travelled by solvent = 14.9 cm Distance travelled by solute= 9.5 cm
Q: If you wanted to make a red object look the same as a black object, which filter (or filters) would…
A: A filter is transparent material that absorb some colour and allow the other colours to pass .…
Q: A student ran a TLC plate using a 90:10 hexane:ethyl acetate developer and this was the outcome. How…
A: Given that , A student ran a TLC plate with 90:10 of n-hexane : Ethyl acetate.
Q: From the experiment up what are possible reasons that make the food dyes diffuse to form distinct…
A: Diffusion is a very slow process. Processes like agitation or increasing temperature can increase…
Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 13.8 cm and the spot…
A: In the given TLC experiment the movent of the solvent is = 13.8 cm The movement of the spot is = 6.5…
Q: A student measures the solvent height on a TLC plate as 11.5 cm. Spot A was measured at a height of…
A: Formula used :- RF = distance traveled by compound/distance traveled by solvent
Q: Although TLC is qualitative and tells you what is present in a given sample, it can also be used to…
A: Thin layer chromatography (TLC) is a part of types of chromatography techniques that is used in the…
Q: Question: Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with…
A:
Q: R Spectrum and analysis of peaks and Possible causes of error
A: A question based on IR spectrum that is to be accomplished.
Q: In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If…
A: To ensure that the colours separate into independent bands that do not overlap as they migrate up…
Q: technique called “GCMS” is often used instead of simple GC analysis. This technique entails passing…
A: GC stands for gas chromatography. It is a chromatography technique used in chemistry. MS stands for…
Q: As distillation method is used to seperate a homogenous mixture, which kind of mixtures…
A: Chromatography is a technique that is used for separating the components in a mixture. Separation is…
Q: What is wrong with this picture? TLC PLATE
A: Given a TLC paper
Q: Describe one alternative column other than a C18 for caffeine detection in terms of stationary phase
A: C18 columns are present in high performance liquid chromatography (HPLC) that are utilized as a…
Q: In analytical chemistry, when and why do we do representative sampling? How can you apply this…
A: A question based on analytical chemistry, which is to be accomplished.
Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 14.5 cm and the spot…
A:
Q: Calculate the Rf value of a spot if it travelled 1.5 cm while the solvent travelled 2.5 cm. Express…
A: Given : 1. Spot distance = 1.5 cm 2. Solvent distance = 2.5 cm
Q: Column chromatography is also very useful for separating mixtures of organic compounds that are not…
A: Column chromatography is one of the most useful methods for the separation and purification of both…
Q: Calculate the RF value for a spot in a TLC experiment if the solvent move 13.5 cm and the spot move…
A:
Q: [1] In paper chromatography, why do some ions move only a short distance from the point of…
A: In paper chromatography, the stationary phase is the water present in trapped state between the…
Q: Preparing a Thin Layer Chromatography experiment there are some common mistakes. Explain what…
A: Interpretation- To tell about all the common mistakes when we prepare a thin layer chromatography…
Q: When using cuvettes to measure the absorbance of a solution, why is it necessary to only hold the…
A: Generally during the measurement of absorbance of a solution ,we hold the cuvettes near the top ,due…
Q: Chemical dyes are used to increase resolution of objects. True False
A: Chemical dyes are used to increase resolution of objects. This statement is true or false is the…
Q: B Given the chromatogram from a simple paper chromatography experiment, which statement is TRUE? O…
A:
Q: What are the main components of a spectrophotometer? Choose all that applies. radiation source…
A:
Q: A gel filtration column has a radius (r) of 0.80 cm and a length (l) of 20.0 cm. Calculate the total…
A:
Q: the specific rotation
A:
Q: How can TLC be used to determine if a sample is pure?
A: Thin Layer Chromatography (TLC) is a separation procedure that requires a very small sample. It is…
Q: 1) Determine the total volume of the standards by using a formula in the appropriate boxes (your…
A: The data is given for absorbance versus concentration of different solutions.
Q: What are some ways to visualize a TLC plate? Why may it be necessary to have more than one method to…
A: TLC works on the basic principle of separation of compounds based on the interaction of the sample…
Q: From the experiment in Question 1, the following data were obtained: 2. Distance traveled by spot O…
A:
Q: The TLC and mp data contradict one another with regards to purity. True False
A:
Q: Explain why the dye spots are elongated and not round on your chromatogram.
A: The term chromatography or the paper chromatography refers to a technique which is used for the…
Q: What would happen to the retention time of a compoundi
A: Retention time is defined as the time that is by the solute to pass through a column during…
Q: In a 2D experiment, what is sandwiched between two evolution and detection processes? O 1.…
A:
Q: Calculate the Rf value for a spot in a TLC experiment if the solvent moved 13.5 cm and the spot…
A:
Q: There were 2 spots on the TLC that did not match up with any of the standards. What were the Rf…
A: A TLC plate is used to determine whether a reaction is proceeding or not. This is done by seeing the…
Q: what chromatography seperation how is tlc part of it
A:
Q: Question 9 Which of the following is not a part of conventional UV/Vis. spectrophotometer? A…
A: UV/Vista spectrophotometer is used in UV/Vista spectroscopy.
Q: What is affinity chromatography? What 'are the principles of affinity chromatography? which…
A: Answer - Affinity chromatography - Affinity chromatography is a separation method based on a…
Q: What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a…
A: Introduction: Chromatography is a technique used to separate the mixture if solution. There are two…
Q: Two compounds have Rf values of 0.50 and 0.61, respectively. The solvent front on a plate is…
A: Term Rf value is related to chromatography technique. Rf value is the distance travelled by given…
Q: A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only…
A: TLC is thin-layer chromatography which involves distribution of a solid sample by solid-liquid…
A student performs a TLC experiment and at the end of the experiment, sees no spots other than the initial spot. What does this mean, and what should the student do to fix the problem?
Trending now
This is a popular solution!
Step by step
Solved in 3 steps with 1 images
- When do you need to blank a spectrophotometer (Spec 20)? After completing all data collection. After the wavelength is changed. Before running a set of samples. Between each sample, even if the wavelength remains the same.Which is TRUE for spectroscopy of a mixture? A. The absorbance for a mixture at a particular wavelength is the sum of the absorbances for the components that absorb at the particular wavelength. B. Spectrophotometers can differentiate between mixture components that absorb at the same wavelength. C. Each component in a mixture has the same molar absorptivity at the same wavelength. D. The concentration for each component in a mixture is easily calculated by least squares for guesses of each component when the individual spectra are well resolved.Cite one research article that used an IR spectrophotometer in their study and discuss how they used the instrument to generate their data and how they used the data of IR in answering their hypothesis.
- Kiko wants to determine the concentration of an unknown solution which produces a green solution. However, the spectrophotometer he uses doesn't have a function to determine the analytical wavelength of the solution. What is the most probable analytical wavelength he can use for the experiment? Refer to the color wheel below.A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A. 52 B. 3.1 C. 2.4 D. 0.52 E. 0.031Why do we need to prepare a standard curve before we can use Beer's Law to determine the concentration of a solution? O We don't have to create a standard curve so long as we choose the appropriate wavelength. Because the standard curve tells us the absorbance of our sample Because the standard curve is used to determine the extinction coefficient Because the standard curve indicates what wavelength to set the spectrophotometer to
- Force Completion Once started, this test must be completed in one sitting. Do not leave the test before clicking Save and Submit Remaining Time: 36 minutes, 58 seconds. Question Completion Status: A Moving to another question will save this response. Question 9 Which of the following compounds gives an infrared (IR) spectrum with a peak at 1730 cm (strong peak)? ОН ОН 1 2 3 4 2 3 4 A Moving to another question will save this response. MacBook Air OO0 D00 F4 F2 II F8 F3 F5 F6 F7 F9 $ & 2 5 7 8 tA4 #3Question 4 What is the correct order of the components in a UV-Visible spectrophotometer? A) Sample Light source →→ Monochromator→ Photodetector B Light Source →→→ Sample → Monochromator→ Photodetector C Light source → Sample Photodetector → Monochromator D Light Source →→→ Monochromator→ Sample Photodetector ContinueDoes sunlight affect uv vis spectrophotometer ? is this statement true? ''Sunlight can dim the measurements by heating the instrument, leading to inaccurate measurements.''
- a. When the light source is monochromatic, Beer's Law holds true. b. Beer's Law establishes a relationship between the concentration of a sample and its absorbance, wherein absorbance is the ratio of light leaving the sample to light entering it. c. For solutions that have more than one absorbing species, Beer's Law holds true even if the analytes of interest don't interact.When do you need to blank a spectrophotometer (Spec 20)? a.After the wavelength is changed. b.After completing all data collection. c.Before running a set of samples. d.Between each sample, even if the wavelength remains the same.Which of the following explains why a more concentrated dye solution has a higher absorbance? A. There are more dye molecules in the solution so more isotopes reach the detector. b. There are more isotopes in solution to emit photons. c. There are more dye molecules in solution to emit isotopes. d. There are more isotopes in solution to emit protons. e. There are more dye molecules in the solution to absorb photons.