The protein catalase catalyzes the reaction 2 H,O,(aq) — 2 H₂O(1) + O₂(g) S and has a Michaelis-Menten constant of KM = 25 mM and a turnover number of 4.0 × 107 s-¹. The total enzyme concentration is 0.010 µM and the initial substrate concentration is 4.83 µM. Catalase has a single active site. Calculate the value of Rmax (often written as Vmax) for this enzyme. Rmax = Calculate the initial rate, R (often written as Vo), of this reaction. R = x10 mM-s-1 mM-s-1

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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The protein catalase catalyzes the reaction
2H,O,(aq) — 2H,O(l) + O,(g)
and has a Michaelis-Menten constant of KM = 25 mM and a turnover number of 4.0 × 107 s¯¹. The total enzyme concentration
is 0.010 µM and the initial substrate concentration is 4.83 µM. Catalase has a single active site.
Calculate the value of Rmax (often written as Vmax) for this enzyme.
Rmax
Calculate the initial rate, R (often written as V), of this reaction.
R =
×10
mM.s-1
mM-s-1
Transcribed Image Text:The protein catalase catalyzes the reaction 2H,O,(aq) — 2H,O(l) + O,(g) and has a Michaelis-Menten constant of KM = 25 mM and a turnover number of 4.0 × 107 s¯¹. The total enzyme concentration is 0.010 µM and the initial substrate concentration is 4.83 µM. Catalase has a single active site. Calculate the value of Rmax (often written as Vmax) for this enzyme. Rmax Calculate the initial rate, R (often written as V), of this reaction. R = ×10 mM.s-1 mM-s-1
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