Which of the following statements is TRUE of BOTH DNA replication and DNA transcription? During the process, the DNA double helix is separated into single-stranded regions. An intermediate in the process is an RNA-DNA hybrid helix. During the process, only one single-stranded DNA region acts as a template. Amino acids are matched to a strand of DNA to form a new polymer.
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- How does DNA replication occur in a precise manner to ensure that identical genetic information is put into the new chromatid? See Figures 8.12 and 8.13. FIGURE 8.12 In DNA replication, the two polynucleotide strands uncoil, and each is a template for synthesizing a new strand. A replicated DNA molecule contains one new strand and one old strand. This mechanism is called semiconservative replication. FIGURE 8.13 A close-up look at the process of DNA replication. (a) As the strands uncoil, bases are added to the newly synthesized strand by complementary base pairing with bases in the template strand. The new bases are linked together by DNA polymerase. (b) DNA synthesis can proceed only in the 5 3 direction; newly synthesized DNA on one template strand is made in short segments and linked together by the enzyme DNA ligase.What is the difference between the leading strand and the lagging strand in DNA replication? There are different DNA polymerases involved in elongation of the leading strand and the lagging strand. The leading strand is synthesized continuously in the 5' → 3' direction, while the lagging strand is synthesized discontinuously in the 5' → 3' direction. The leading strand requires an RNA primer, whereas the lagging strand does not. The leading strand is synthesized in the 3' → 5' direction in a discontinuous fashion, while the lagging strand is synthesized in the 5' → 3' direction in a continuous fashion.DNA replication occurs by adding (Note: NTPS = nucleotide triphosphates; dNTPs = deoxynucleotide triphosphates) DNTPS to the 3' end of the template strand NTPS to the 3' end of the daughter strand DNTPS to the 3' end of the daughter strand DNTPS to the 5' end of the template strand NTPS to the 5' end of the daughter strand
- "Unlike what happens in DNA replication, where both strands are copied, only one of the two strands is transcribed into MRNA. The DNA strand that contains the gene is sometimes called the sense strand, or coding strand, and the DNA strand that gets transcribed to give RNA is called the antisense strand, or noncoding strand. Because the sense strand and the antisense strand are complementary, and because the DNA antisense strand and the newly formed RNA strand are also complementary, the RNA molecule produced during transcription is a copy of the DNA sense strand... The only difference is that the RNA molecule has a U everywhere the DNA sense strand has a T." Consider the following segment of a DNA sense strand: (5') CAA-ACT-ACG-GCG-TTG-CAG (3’)Shown below is a drawing showing the result of an experiment in which an RNA molecule is allowed to mix with genomic DNA that has been denatured by boiling, and the two molecules are allowed to hybridize. The DNA strand is presumed to be the lighter-shaded one on the top. Note that only one strand of DNA is shown. This result was the first evidence for which of the following processes? a Replication b Transcription c Translation d SplicingWhich of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision Repair (BER) is true? Only NER involves the action of DNA ligase to seal nicks in the DNA backbone. Both NER and BER involve the creation of an apyrimidinic (AP) site. Both NER and BER involve a single DNA strand cleavage by an endonuclease. Only BER requires DNA polymerase. Both NER and BER can be activated by exposure to visible light.
- Which of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision Repair (BER) is true? Only NER involves the action of DNA ligase to seal nicks in the DNA backbone. Both NER and BER involve DNA strand cleavage by an endonuclease. Both NER and BER can be activated by exposure to visible light. Only BER requires DNA polymerase. Both NER and BER involve the creation of an apyrimidinic (AP) site.DNA polymerase occasionally incorporates the wrong nucleotide during DNA replication. If left unrepaired, the base-pair mismatch that results will lead to mutation in the next replication. As part of a template strand, the incorporated wrong base will direct the incorporation of a base complementary to itself, so the bases on both strands of the DNA at that position will now be different from what they were before the mismatch event. The MER-minus strain of yeast does not have a functional mismatch excision repair system, but it has normal base excision repair and nucleotide excision repair systems. Which of the following statements is correct about differences in the mutation spectrum between MER-minus and wildtype yeast? More than one answer is correct. Options: More point mutations will arise in MER-minus yeast. Fewer point mutations will arise in MER-minus yeast as compared with wildtype. Of the total point mutations that…During replication of a DNA molecule, the daughter strands are not produced in exactly the same manner. One strand, the leading strand, is made continuously toward the replication fork, while the lagging strand is made in fragments in the opposite direction. This difference in synthesis of the two strands is the result of which of the following? O a. DNA polymerase is not efficient enough to make two "good" strands of DNA O b. The two template strands are antiparallel, and DNA polymerase can only synthesize DNA in the 5' to 3' direction O c. The lagging strand is the result of DNA breaks caused by exposure to UV light Od. The cell does not contain enough nucleotides to make two complete strands Oe. Both B&D
- Match the following descriptions with the enzymes involved in DNA replication. Adds an RNA primer to begin elongation Removes the RNA primer from the beginning of the newly constructed strands Splices lagging strand segments Cleaves the rung of the DNA double helix ladder Description: Answer Answer Answer Answer Enzyme: Helicase DNA Polymerase Primase DNA LigaseName and describe the first four events (and associated enzymes) that happen during DNA replication at the DNA double helix (in correct sequence)Assume a deletion occurs in a gene that encodes DNA polymerase I and no functional DNA polymerase I is produced. What will be the most likely consequence of this mutation? The DNA would not exist in a supercoiled state. There would be no RNA primers laid down. The DNA will not be able to unwind to initiate replication. The DNA strands would contain pieces of RNA. There would be no DNA replication on the leading or lagging strands.