The enzyme invertase is usually found outside the cells of yeast. the enzyme also has 3 carbohydrates on it. In a particular mutant strain, the carbohydrates are present, but the enzyme is found in the rough ER instead of outside the cell. This mutant is defective in O Transport from Rough ER to Smooth Er Transport from Golgi to the outer membrane Transport from the Rough ER to Golgi Transport from Rough ER to lysosomes
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- A bacterial lactose transporter, which is highly specific for lactose, contains a Cys residue that is essential to its transport activity. Covalent reaction of N-ethylmaleimide (NEM) with this Cys residue irreversibly inactivates the transporter. A high concentration of lactose in the medium prevents inactivation by NEM, presumably by sterically protecting the Cys residue, which is in or near the lactose-binding site. You know nothing else about the transporter protein. Suggest an experiment that might allow you to determine the Mr of this Cys-containing transporter polypeptide.Digoxin, a toxin derived from the foxglove (shown), can be used to treat heart disorders such as atrial fibrillation. Digoxin's mechanism of action is to inhibit the Na+/K+ ATPase. Which of the following is the most likely side effect of Digoxin treatment? O Failure to transport glucose into cells while fasting (not eating) O Failure to transport Ht into lysosomes using direct active transport O Failure to transport glucose into cells after a meal O Failure to secrete pancreatic digestive enzymes in response to eatingIn eukaryotic cells secreted proteins are targeted first to the endoplasmic reticulum and then pass through the Golgi, before being released from secretory vesicles into the extracellular space. A much simpler route would be for ribosomes synthesising secretory proteins to be targeted to a translocon in the plasma membrane, with the protein being secreted directly as it is translated. List three potential advantages of the former, more circuitous, route for protein secretion over the simpler, more direct, alternative route suggested.
- "In most animal cells, minus end-directed microtubule motors deliver their cargo to the periphery of the cell, whereas plus end-directed microtubule motors deliver their cargo to the interior of the cell", is true or false.A cell with a K+1 concentration of 0.3 eq/L, and a Na+1 concentration of 0.1 mol/L is placed into a solution with a K+1 concentration of 0.2 eq/L, and a Na+1 concentration of 0.2 mol/L. It is assumed that the K+1 and Na+1 can both pass through the cell membrane (the K+1 and Na+1 transport proteins are open). Answer the following true or false questions. 1. Diffusion of a solute through the membrane is called dialysis 2. K+1 will dialyze (diffuse) out of the cell 3. Na+1 will dialyze (diffuse) into the cell 4. Like the diffusion of gases, diffusion of particles in a liquid is independent of the other solutesIn the absence of tension, MscL channel has an energy gap separating the closed and open states Eo = 50 kT. MscL opening is associated with a 20 nm2 in-plane protein expansion (DA). Using Excel plot its open probability (Po) as a function of membrane tension and paste the graph below. What will be the open probabilities of two MscL channels if one situates at the pole of the bacterial cell above, another in the middle?
- Maintaining nuclear RanGTP levels is critical for nucleo-cytoplasmic transport. Describe how mutations that disrupt RanGEF function would affect the movement of proteins coming in and going out of the nucleus.Certain cellular components appear to move bidirectionally on microtubules. Describe how this is possible given that microtubule orientation is fixed by the MTOC.Part 2A: Preparation of solutions necessary for the investigation of transmembrane transport in yeast In Part 2A, you will make the solutions necessary for Part 2B in the following lab session. 56 mM glucose (Fisher Chemical Company, Waltham, MA) 20 mM HEPES (Fisher Chemical Company, Waltham, MA) pH 6.8 Store this at 4 degrees until the day of the experiment. 03. Determine the total amount of YGM solution you'll need next week in Part 1B, and add to that a good amount extra to be prepared for repeats. Describe in your lab notebook how much YGM your group will likely need AND your exact plan for making it. Tip: Work through the procedure, find where you need YGM and take note of how many experiments you will be doing!
- Which statement about the M6P receptor is true/accurate? Answers: It is involved in retrieval of ER resident proteins from the Golgi and transport back to the ER. Due to no difference in pH in the lumen of each, it has the same affinity for M6P in the Golgi as in the endosome. Due to a slight difference in pH in the lumen of each, it has a high affinity for M6P in the Golgi and a low affinity for M6P in the endosome. Due to a slight difference in pH in the lumen of each, it has a low affinity for M6P in the Golgi and a high affinity for M6P in the endosome.Transport of histidine into a bacterial cell was measured at several different histidine concentrations, see table below. Based on these data, is histidine uptake facilitated by a carrier or channel protein? a b Histidine (uM) 2.5 7 16 31 72 с Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. A carrier protein because it becomes saturated. A carrier protein because it does not become saturated. A channel protein because it becomes saturated. Transport (uM/min) d A channel protein because it does not become saturated. 42.5 119 240 490 1000 X Your answerDrag the terms on the left to the appropriate blanks on the right to complete the sentences. Terms can be used once, more than once, or not at all. Submit dCTP GMP AMP GTP dTTP activation inhibition dGTP My Answers Give Up UTP + Gln + ATP → CTP + Glu+ ADP + P₁. Cofactor: dCMP + H₂O →→→→DUMP + NH3. Cofactor: CDP+ [2H] → dCDP+ H₂O. Cofactor: Effect: Effect: Effect: Reset Help