the enzyme and substrate should be mixed only when the reaction is initiated (when the absorbance readings are taken) the michaelis constant must be known

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Which of the following steps should be followed
when performing kinetics experiments?
Choose all that apply, partial positive and
negative mark are given for correct and
incorrect answers.
enzyme concentration is kept constant
for all reactions
the enzyme and substrate should be
mixed only when the reaction is
initiated (when the absorbance
readings are taken)
the michaelis constant must be known
reaction rates must be taken until
reaction completion
Transcribed Image Text:Which of the following steps should be followed when performing kinetics experiments? Choose all that apply, partial positive and negative mark are given for correct and incorrect answers. enzyme concentration is kept constant for all reactions the enzyme and substrate should be mixed only when the reaction is initiated (when the absorbance readings are taken) the michaelis constant must be known reaction rates must be taken until reaction completion
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