Question 18Which of the following activities of DNA pol I is MOST important in proofreading?A) 5' to 3' exonucleaseB polymerase activityligation activity(D) 3' to 5' exonuclease

The DNA replication in the newly added base are read by DNA pol I enzyme to check weather the added…

Answered: Question Which of the following…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question 11 Each cycle of amplification in PCR involves all of the steps EXCEPT: A) annealing of oligodeoxyribonucleotide primers to DNA. B) the addition of fresh dNTPs to the reaction mixture. C reaction with DNA polymerase at approximately 70°C. D) thermal denaturation of the target duplex DNA.
QUESTION 21 Choose each of the characteristic(s) of all RNA polymerases from the list below. A. elongation adds new ribonucleotides to the 3-OH B. requires a primer to initiate synthesis C. have 3' to 5' exonuclease activity D. adds nucleotides based on sequence complementarity to a template E. have 5' to 3' polymerase activity O F. elongation adds new ribonucleotides to the 2'-OH G. have 3' to 5' polymerase activity
Question 5 Review Figure 21.6 types of DNA sequences in the human genome. The lowest percentage of human genome O repetitive DNA related to transposable elements and related sequences (L1 sequences and Alu elements) O repetitive DNA unrelated to transposable elements (simple sequence and large-segment duplications O unique noncoding DNA O introns O exons O regulatory sequences
Question 30 Which of the following correctly describes a difference between RNA & DNA polymerases? (A) RNA polymerases usually do not need a template, while DNA polymerases do. B DNA polymerases usually require a primer while RNA polymerases do not. RNA polymerases usually synthesize introns, while DNA polymerases synthesize cistrons. D RNA polymerases polymerize 5' –> 3', while DNA polymerases polymerize 3' –> 5'.
Question 4. Given the probability that a restriction enzyme will cut DNA if the recognition sequence for the enzyme is 5'-GGATCC-3' and the GC content is 60%, c) Which of the following will lead to DNA cutting more frequently? Choose all that apply. O Increasing the AT content to 60% O Using a seven base cutter with the sequence 5'-GGATCCC-3' O Using a five base cutter with the sequence 5'-GGACC-3' O Increasing the GC content to 70% O Decreasing the GC content to 50%
Question 16 Advantages of using the PCR include the following EXCEPT A it will always give the desired product B it can target desired sequences from either genomic or cDNA templates (C) the reaction is specific to a desired DNA sequence (D) only small amounts of template are needed
Question 19 The polymerase chain reaction requires the following except A primers complementary to the ends of the sequence to be amplified B) carefully controlled temperature conditions cycles of denaturation, annealing and extension RNA polymerase to synthesize RNA primers
Question 12 Which of the following DNA pol I activities would be MOST important in the removal of the primer? A 3' to 5' exonuclease activity B 5' to 3' exonuclease activity polymerase activity D) ability to nick both strands of the DNA
Question 8 The cloning a eukaryotic gene in a bacterial plasmid does not include extension gene inserted into a cloning vector such as a plasmid The vector is obtained by bacterial cells. O Host cells grown in culture to form a clone of cells containing the "cloned gene of interest" O formation of a recombinant DNA
Question 14 Why are long reads the preferred method to sequence repetitive elements? Select the statement that is FALSE. ◇ If a repetitive region is longer than 300 basepairs (the length of a short read) it's impossible to determine the total length. O Long reads are more likely to span repetitive regions on either side, making it easier to map the repetitive regions. O It's hard to determine where short reads should be placed, as they could map to multiple repetitive regions. O Long reads are less error prone and thus better for sequencing overall. Question 15 Which of the following statements is NOT true about GWAS? When identifying alleles associated with a disease, we compare the frequency of an allele in a control group versus a cash group
QUESTION 19 Which of the following is/are true regarding next generation sequencing? Next generation sequencing is also known as 'sequencing by synthesis' Next generation sequencing uses ddNTPS to identify the base at the end of a nucleotide chain Next generation sequencing will NOT work if chain terminating nucleotides are included in the reaction Next generation sequencing uses fluorescently labeled ddNTPs instead of radioactively labeled ddNTPs Next generation sequencing allows hundreds of millions of DNA fragments to be sequenced at the same time QUESTION 20 tick Save and Submit to save and submit. Click Save All Answers to save all answers.
Question 25 If one DNA segment has the following base composition, 5-CAGTTAGTCA-3', which of the following sequences is complementary? A 3'-CAGTTAGTCA-5' 3'-TGACTAACTG-5' 3-TGACTAAСTG-5 D 5-TGACTAACTG-3'

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Question Which of the following activities of DNA pol lis MOST important in proofreading A 5' to 3' exonuclease B polymerase activity ligation activity 3' to 5' exonuclease