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Genetics Question 2
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- Question 5 Okazaki fragments are short DNA pieces that explain how the DNA polymerase can continue the synthesis of the new strand. A) True B) FalseQuestion 2. Ribosomes are cellular structures that are composed of protein and RNA; this structure is responsible for catalyzing peptide bond formation between amino acids during a process known as translation. a) Many antibiotics that kill bacteria target translation. Why might this be an effective mechanism to kill bacteria? Why don't antibiotics also kill human (eukaryotic) ribosomes? b) The antibiotic Kasugamycin (KSG) destabilizes the P-site of the ribosome. Describe what parts of translation would be altered in the presence of this antibiotic. c) How does the following graph show the efficacy of translational knockdown with KSG? Met-Methionine C % of Met incorporation 100 80 60 40 20 0 + 0 2 4 6 8 KSG concentration (mg/ml) 10Question 33 RNA polymerase IlI: A is located in the nucleoplasm and transcribes the protein-encoding genes through mRNAs. B transcribes tRNA genes and protein transport genes. c) transcribes the 55 RNA genes. D) transcribes RNA genes associated with TRNA processing.
- Question 6 The helical turns of the DNA does not only provide spaces for binding with regulatory proteins but they cause the molecule to resemble a spiral staircase. A) True B) FalseQuestion 18 Which of the following activities of DNA pol I is MOST important in proofreading? A) 5' to 3' exonuclease B polymerase activity ligation activity (D) 3' to 5' exonucleaseQuestion 7 A unique 5'CCA terminal sequence is found in all TRNAS to be able to carry the correct amino acid to the ribosome. (A) True B) False Question 8 Predominant nucleotides during protein synthesis are the GTPS. A True B) False Question 9 The nucleoside monophosphates are seen in metabolic pathways because their phosphoric anhydride bonds can be hydrolyzed off for energy. (A True False Question 10 The cyclic form of the sugar in DNA is a beta-D-3-deoxyribofuranose. A True False
- QUESTION 15 Consider the gene that encodes telomerase RNA. Suppose that the sequence of the template strand that spans the region used to synthesize the telomere repeat has a sequence 3'-GGAACC-5'. If so, what will be the sequence of the telomere? A. 5'-CCAAGG-3' O B. 5'-GGAACC-3' O C. 5'-CCTTGG-3' O D. 5.CCUUGG-3' O E. 5'-GGTTCC-3'Question 1. Enzymes, proteins and deoxyribonucleic acid (DNA) macromolecules. Enzymes are not only speed up the reaction, but also are necessary for DNA repreduction. are important biological a) Compare the process of protein synthesis between eukaryote mRNA and viral RNA b) With the aid of a diagram, draw an adapter molecule that recognizes the codons of mRNA and explain its functions in DNA translation.Question 1 In prokaryotes, the sigma factor recognizes base sequences in the which facilitates RNA polymerase binding and initiates RNA synthesis. A operator B promoter c) regulatory sequence D) enhancer
- Question 43 The addition of restriction endonucleases in the cloning process is done following the ligation with DNA ligase. A) True B) FalseQuestion 12 The "natural" function of restriction endonucleases is to A help bacteriophages infect cells. B) regulate gene expression from specific promoters. protect bacterial cells which have them. D) remove chromatin from histones.Question 14 During RNA chain elongation gyrase proceeds ahead of the transcription bubble in order to A remove positive supercoils by a cut and seal process B remove positive supercoils by creating relaxed DNA c remove negative supercoils by removing H-bonds D) remove negative supercoils by unwinding the 2 strands