Per the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a standard plate count. You are curious if the fresh eggs that you buy from your neighbor are considered safe to consume so you use your eScience Microbiology kit to test these eggs using direct plate count after serial dilution. After you complete the experiment, you obtain 74 countable colonies from the 10-2 dilution plate. The inoculum volume you plated was 0.1 mL.   How many bacteria are present in 1 mL of the egg you sampled?   Are these eggs considered safe to consume per USDA standards?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Per the USDA, whole, unpasteurized fresh eggs can contain no more than 50,000 CFU/mL bacteria in a standard plate count. You are curious if the fresh eggs that you buy from your neighbor are considered safe to consume so you use your eScience Microbiology kit to test these eggs using direct plate count after serial dilution.

After you complete the experiment, you obtain 74 countable colonies from the 10-2 dilution plate. The inoculum volume you plated was 0.1 mL.

 

  1. How many bacteria are present in 1 mL of the egg you sampled?

 

  1. Are these eggs considered safe to consume per USDA standards?
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