For the pour plate method, state which plate will be chosen for colony isolation and why? From the chosen plate, indicate (circle) the colonies for both bacterial species that will be selected and transferred to new nutrient agars as a pure culture.

Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…

A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…

Q: Can gelatin hydrolysis be correlated with the pathogenicity of a bacterium? Explain your answer

A: Disclaimer: “Since you have asked multiple questions, we will solve the first question for you. If…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: In what instances would the pour plate method be more appropriate than the spread plat method?

A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: Why is the culture medium referred to as a highly enriched laboratory soil?

A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…

Q: aseptic technique and pure culture technique

A: A‌SEPTIC TECHNIQUE_ *Inserting PICC lines. *Performing dialysis.‌ *Inserting catheters.‌ *Running…

Q: Identify one culture-based and one non-culture-based method used for analyzing bacterial growth.

A: Bacteria divide by binary fission. They grow in an exponential manner. Four different phases can be…

Q: why it is useful to plot the microbial growth from a growing microbial culture?

A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…

Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…

A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…

Q: If you transfer 0.1 mL of culture into a 99mL of sterile water, then add 1mL of that to an agar…

A: Making a solution thinner or less dense is the procedure of dilution. Serial dilutions (also known…

Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…

A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: what tool is used to inoculate the agar surface when careying out the antibiotic sensitivity test…

A: Antibiotic sensitivity test is a microbiological test to know the effect of antibody on microbes…

Q: temperature and light source affect the number and type of colonies that can be found in the…

A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…

Q: What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for…

A: Enrichment culture media is the one which is used for the growth and isolation of a specific type of…

Q: What is a pure culture and how can one be obtained

A: Cells are the structural and functional units of life.

Q: On which of the following types of media would you expect untransformed bacteria to grow on?

A: In this question, we have to answer which kind media support the growth of untransformed bacteria.

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: According to these images and your own knowledge, can you make a biological drawing of bacteria in…

A: Yoghurt is a nutrient which is rich in lactobacillus (lactic acid bacteria) . Convert milk into…

Q: which method do you think is more reilable, a test utilizing microbial growth media, or a…

A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…

Q: If there is 50 bacterial colonies on a 1:1000 dilution streak plate. How many cfu/mL are there?

A: Introduction: A CFU stands for colony-forming units. It is a unit that we use for estimating the…

Q: Describe three problems associated with using the standard plate count method for determining the…

A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…

Q: Given the scenario, compute for the total volume of the culture media solution (milliliter or liter)…

A: We have been given the concentration of the nutrient broth and Agar and we have also been given the…

Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…

A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…

Q: why In spread-plate technique, the bacterial suspension volume  should not exceed 0.1ml.

A: Spread plate method is a technique which uses a Petri plate or dish in which a growth medium or gar…

Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…

A: Bacteria from colonies in the agar plates and different bacterial colonies will differ in shape,…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: What is the main difference between plates before and after centrifugation? What may be the purpose…

A: Answer: CENTRIFUGATION = It is the process involving mechanical force that is centrifugal force,…

Q: List the step-by-step procedure of Gram staining.

A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…

Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…

A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…

Q: What adjustments can be made to the formulation of media used for enumeration of yeasts and moulds…

A: Microbial culture is an essential method for the study of them in the lab. Proper vigil is needed in…

Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…

A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…

Q: What are the advantages and disadvantages of using the Slide Culture technique?

A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…

Q: Assuming you had a bacterial culture with a concentratoion of 2x10^9 bacteria/mL, whaat volume would…

A: A microbiological culture, also known as a microbial culture, is a technique for growing…

Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…

A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…

Q: a single agar plate can be used to quantitatively measure the MIC value of multiple antibiotic…

A: Antibiotics are a typical type of antibiotic that doctors use to treat bacteria. Antibiotics are…

Q: A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts.…

A: When the culture medium is dispersed on the viable plate, a very thin layer is formed in which…

Q: The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in…

A: Acid fast bacteria are those which responds to acid fast staining . Most of the members of…

Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…

A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.

Q: Why does a streaking method used to inoculate plates result in isolated colonies? (Explain…

A:

Q: Describe the difference between batch and continuous culture techniques

A: Bacteria and fungi, for example, are extremely valuable to a wide range of industries.…

Q: How many different types of colonies can you find on the blood agar plate? describe each. (As shown…

A: Blood agar is general medium that is provided with the nutrients and can be use as a general growth…

Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: Briefly, in your own words, describe the steps of the gram staining procedure.

A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…

Q: Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind…

A: The selective ingredients of MSA the bile salts and the dye, crystal violet which inhibit the growth…

Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…

A:

Q: You have performed an experiment and your aim is to obtain pure colonies. However, you observe some…

A: Bacteria are prokaryotic organisms. They are microscopic single-celled organisms that can live in a…

Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…

A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.