In the traditional alkaline lysis method, what is the purpose of Solution 1? For what is its use?
Q: ain... My Drive - Google... 4. Nucleotides within a single DNA strand are connected together by…
A: 4. Answer: Nucleotides within a single DNA strand are connected together by phosphodiester linkages,…
Q: Based on your knowledge of the natural amino acids, what would you expect pKa of the side chain of…
A: Succinic acid have 4 carbons, among which the two terminal carbons are carboxylic acid groups. The…
Q: 7. You have a 200 mg/mL stock solution of protein. To make 20mL of 5mg/mL solution, add of stock and…
A: Dilution results in a decrease in the concentration of a substance in a mixture. A stock solution is…
Q: 2.4. the second stage of cholesterol synthesis further conversion into cholesterol; formation of…
A: “Since you have posted multiple questions, we will provide the solution only to the first question…
Q: Human insulin is prodụced as a recombinant protein in the yeast Saccharomyces cerevisiae The protein…
A: A glycosylated protein is a protein that has a carbohydrate moiety attached to it. Given to us are…
Q: Calculate the pI of the following peptides: a. MASTERY b. STICKY c. DALLAS d. ART
A: the isoelectric point of a protein is the pH at which the net electric charge of that amino acid is…
Q: Assuming the average weight of a deoxynucleotide monophosphate (dNMP) is 327.0 g/mol, how many…
A: Nucleic acids primarily act as transmitters and stores genetic information. DNA is the chemical…
Q: Write the line structure of each of the following peptide at pH7 and identify how many peptide bond…
A: To calculate the number of peptide bonds in a peptide, you need to know the number of amino acids it…
Q: Question: A man inspires 1000 ml from a spirometer. The intrapleural pressure was 24 cm H2O before…
A: The compliance of a system is defined as the change in volume that occurs per unit change in the…
Q: Optional) Describe the structure of DNA, discussing the DNA helix and the base pairs of ONA, the…
A: Introducion There are two types of nucleic acid present in our body. DNA and RNA. DNA acts as a…
Q: Deficiency in HGPRT will lead to what? a. No synthesis of ATP b. No salvage pathway for all purines…
A: Hypoxanthine- guanine phosphoribosyl transferase (HGPRT) is required in salvage pathway which is…
Q: Compare and contrast Pyruvate Dehydrogenase with a-ketoglutarate dehydrogenase Outline the…
A: The tricarboxylic acid cycle is the metabolic pathway that generates NADH and FADH2 from the…
Q: Inhibitors are important in developing new pharmaceuticals. Two important classes of inhibitors…
A: Inhibitors are molecules or compounds that reduce the rate of a chemical reaction, or block it…
Q: Identify what GalNAc, GlcNAc, Frutose and Sialic Acid are, draw them and describe their…
A: Carbohydrates chemically are polyhydroxy aldehydes or ketones. They serve diverse function roles…
Q: 1. Bacteria have a membrane potential, although the mechanisms of how it is maintained differ from…
A: To calculate the number of positive ions needed on the exterior surface of the bacterium to…
Q: Meiotic crossing over occurs between: Selected answer will be automatically saved. For keyboard…
A: Meiosis occurs in the sexually reproducing cells called gametes in higher organisms. A gamete…
Q: 6. Retroviruses have an RNA genome instead of a DNA genome, and the RNA must be copied into the…
A: Retroviruses are a type of virus that has a unique way of replicating their genetic material. Unlike…
Q: Which one of the following concerning fermentation is correct? Kimchi's high salt content prevents…
A: Fermentation is a metabolic process in which microorganisms such as yeast and bacteria convert…
Q: states the five functions of a globular protein and an example for each.
A: Proteins are large biomolecules composed of amino acids linked by peptide bonds. They play a vital…
Q: n a-c below, will the residue on the right-hand side increase or decrease the pKa of the residue on…
A: Amino acids that do not have ionisable side chains are zwitterion at neutral pH since they have an…
Q: A genetically modified bacterium was cultivated to produce 1,3-propanediol (1,3-PDO), and the…
A: Oxygen Demand is a measure of the amount of oxidizable substances in a water sample that can lower…
Q: Why are hydrogenases oxygen sensitive? Consult Morra, S. (2022), Fantastic [FeFe] Hydrogenases and…
A: Hydrogenases are enzymes that catalyze the oxidation and reduction of molecular hydrogen. They play…
Q: Name : Surname: 2. The amino acid side chain residues in an a helix point center of the helix. A)…
A: The proteins consist of 20 naturally occurring amino acids. The proteins must fold into the proper…
Q: 1)s. Name an enzyme that requires NAD/NADH for activity. Draw the chemistry of this reaction (show…
A: Enzymes are highly specialized proteins that have extraordinary catalytic power, greater than that…
Q: 1.2 Ninhydrin Test Substance 2% glycine 2% gelatin 2% albumin 1% tyrosine casein + distilled water…
A: Ninhydrin test : It is the alpha-amino groups in proteins and amino acids that are responsible for…
Q: In a case where the energy source to be used it 1 malate unit, exactly how many ATP can be yielded?…
A: Cellular respiration is a collection of three metabolic pathways that generate ATP from oxidation of…
Q: Enzymes are essential to the processes of photosynthesis and cellular respiration. Names two factors…
A: Enzymes are high molecular-weight protein molecules that catalyse biochemical reactions. The…
Q: Identify the principle (what amino acid/s it detects and the general reaction), the reagents used,…
A: Since you have posted a question with multiple sub parts, we will provide the solution only to the…
Q: 12. Which of the following ligands cannot act as an ambidentate ligand? o nitrite, NO₂ o…
A: Ligand is a molecule which bind reversibly to the protein. A ligand may be any kind of molecule, it…
Q: 1. What is Du? ESTIONS ON LABORATORY ASSAY NO.7 2. Why do we need test for Du when weak or no…
A: Since you have posted multiple questions, we will provide the solution only to the first question as…
Q: Consider the following α helix from myoglobin at pH 7.…
A: Proteins are folded polypeptides. Polypeptides are amino acid polymers linked to each other via a…
Q: Isolate B Isolate A Isolate C Isolate D The purity and concentration of DNA isolate can be evaluated…
A: DNA purification is a process used to isolate DNA from other cellular components. The most common…
Q: Bile acids: localization of synthesis, their biochemical significance.
A: Introduction Cholesterol is a compound which is essential for our body. Cholesterol is synthesised…
Q: True or False: The side chain of glutamate can form an ionic bond at pH 1. True False
A: Glutamate is an α-amino acid that is commonly found in proteins.The amino acid has a side chain…
Q: In terms of organism, location and function, what are the major differences between nuclear and…
A: DNA is deoxy ribo nucleic acid. It is a hereditary material and can store genetic information. This…
Q: Deduce the amino acid sequence of a polypeptide from the following: 1. Acid hydrolysis gives Ala2,…
A: Deducing the amino acid sequence of a polypeptide from the analysis of peptide fragments generated…
Q: True and false - A k+ channel will be just as permeable to Na+ as to K+ because Na+ is a smaller ion…
A: Ion channels are proteins that form pores in the plasma membrane and allow ions to pass through in…
Q: You are making 300 mL of MS-agar medium for plant tissue culture. You need 0.4 mg/LBAP and 0.8 mg/L…
A: Recall the equation of dilution: C1 x V1 = C2 x V2 Where, C1 is the concentration of the stock…
Q: The hormones insulin and glucagon play an important role in the regulation of plasma glucose. a)…
A: Our body primarily uses glucose as a source of energy. In order to carry out the normal functions of…
Q: ) Describe the 10 enzymatic reactions of glycolysis and the organization of the pathway in 2 main…
A: Glycolysis is a metabolic pathway during which glucose molecule splits into pyruvate molecules with…
Q: Part Three: Metabolism: Your cells are going to USE the food they've been provided! Describe the…
A: Metabolism is the chemical reactions occurs in our body which changes the food into energy to run…
Q: Time (s) 200 180 160 140 120 100 80 60 40 20 0 0 Change in absorbance vs Time 0.05 y = 660.47x…
A: Change in absorbance per minute at any wavelength can be determined by using the ratio of…
Q: 1. In the figure shown, identify what is labeled by the letter C. * A 0- D 0=P-O-CH₂ сн, В 0- Ribose…
A: Nucleotides are phosphoric acid esters of nucleosides. They are the building blocks of nucleic…
Q: 1. Why is it necessary to calibrate a Pasteur pipette? 2. Cite some possible sources of errors in…
A: Since you have posted multiple questions, we will provide the solution only to the first question as…
Q: The question stated the standard free energy change not the actual free energy change. You solved…
A: Standards free energy change calculated at biochemical standards is called biochemical standard free…
Q: Why would LDH be up- or down-regulated depending on location of cancer cells? Anaerobic cancer…
A: Introduction glycolysis is a process by which glucose breaks into pyruvate. Glycolysis occurs both…
Q: Consider a peptide with the sequence Ala-Glu-Arg-Leu. Assume the ionizable groups have the pKa…
A: Amino acid sequences are written with N-terminal amino acid on the left and C-terminal amino acid on…
Q: Explain why the fluorescence signal decreases as the concentration of TNT in the sample increases
A: The procedure of immunoassay describes that: TNT-binding antibodies (atb) are chemically attached…
Q: What chemicals do you think are most harmful to your own health? How are you exposed? How could you…
A: Benzene and other aromatic hydrocarbons, along with heavy metal compounds are one of the the most if…
Q: 23. Which is a general term indicating a carbohydrate polymer? A) Glycan B) Polycarb C) Multimer D)…
A: Chemically carbohydrates are polyhydroxy aldehydes or ketones. They have the general formula :…
In the traditional alkaline lysis method, what is the purpose of Solution 1? For what is its use?
Step by step
Solved in 2 steps
- What is demanufacturing, and why is it used for some types of waste?A disk-diffusion test using E. coli gave the following results: Disinfectant Zone of inhibition (diameter in mm) A 15 B 2.0 C 10 D 5 In the table above, which compound was the most effective against E. coli? A) A B) B C) C D) D E) The answer cannot be determined based on the information provided.What criteria are used to determine the specified substrate?
- What are the main types of immobilization techniques used in biosensors? (with a deep explanation)What is Bioreactor? What are the advantages of Stirred tank Bioreactor over Shake flask.The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks) a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w / v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .