In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purple

In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purple

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 17SST

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In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The counterstain (safranin) causes the purple color to develop The primary stain, crystal violet, is dark purple Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol
All of the following are correctly matched and can be found in bacteria EXCEPT: None of the other four answers (all are correctly matched and can be found in bacteria) Nucleus membrane-bound organelle containing multiple chromosomes Ribosomes - site of protein synthesis Cell wall contains peptidoglycan Cell (plasma) membrane - encloses cytoplasm; phospholipid bilayer with proteins
In the Gram stain, gram-negative bacteria appear red for all the following reasons EXCEPT: They stain with safranin because they are colorless at the step where it is added Safranin is a red stain None of the other four answers (all are true) The peptidoglycan layer in their cell walls is too thin to retain the crystal violet-iodine complex when the decolorizer (alcohol) is added The gram-negative cells' outer lipopolysaccharide layer (outer membrane) prevents their being stained by crystal violet in the first step
What color would the bacterial cells have when viewed microscopically after the following treatments: Gram Reaction Ammonium Oxalate Gram lodine Ethyl Alcohol Safranin Crystal Violet Gram-positive Gram-negative
When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone 2-Crystal violet 3-Safranin 4-lodine O 2-1-4-3 2-4-1-3 O 1-2-3-4 O 1-3-2-4 O 4-3-2-1
Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained cells. Choices may be used more than once--------- Pseudomonas aeruginosa after decolorization
Answer this question xi) What is the most important step in the Gram staining procedure? Why?
Put the following Steps of gram smear in order Create a bacteria Add crystal violet to the bacterial smear. Rinse with water after 60 seconds. Allow the smear to air dry and then heat fix it. Add gram iodine to the smear, let it sit for 60 seconds and rinse water decolorize with 95% ethanolfoe 5-10 seconds and then rise with water Blot dry with bibulous paper Add safranin to the smear and let sit for 60 seconds, then rinse with water Observe using oil immersion
You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? Red/Pink Purple colorless
You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purple
Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those bacteria with a thick layer of peptidoglycan have purple appearance in gram dye/stain test. True False