Hii, can anyone help me with this. Your help would be very much appreciated, thank youuu. Below are morphological, physiological and biochemical characteristics of anunknown luminous bacterium isolated from the gills of a marine fish. Use the databelow to identify the isolate. Make an identification scheme.TestUnknown Isolate BLuminescenceCell ShapeGram StainingOxygen RequirementMotilityDrop)Growth atPositiveStraight RodsNegativeFacultative Anaerobe345(HangingMotileNegative0% NaCi73% NaC1Positive86% NaC1PositiveAccumulation of PHBFlagellaOxidasePositive1 PolarPositive9101112Nitrate Reduction13GelatinaseNegativePositiveGrowth at 35°CGrowth at 4°C1415NegativePositiveMethyl RedVoges-ProskauerCatalaseStarch HydrolysisLysine DecarboxylaseIndole ProductionUtilization ofGlucoseLactoseMannitol1617Positive18Positive19NegativeNegativeNegative202122Positive (fermentative)Positive (fermentative)NegativeNegativePositive (fermentative)NegativePositive (oxidative)23242526Sucrose27XyloseInositol2829Galactose

The isolate identified from the above data is Photobacterium. Photobacterium is a free living, gram…

Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme. Test Unknown Isolate B Luminescence Cell Shape Gram Staining Oxygen Requirement Motility Drop) Positive Straight Rods Negative Facultative Anaerobe 2 3 4 (Hanging Motile Growth at Negative 0% NaCi 3% NaC1 Positive 6% NaCl Positive Accumulation of PHB Flagella Oxidase Positive 1 Polar 10 11 12 Positive Nitrate Reduction Negative Positive 13 Gelatinase Growth at 35°C Growth at 4°C Methyl Red Voges-Proskauer Catalase Starch Hydrolysis Lysine Decarboxylase Indole Production Utilization of 14 15 Negative Positive 16 17 Positive 18 Positive Negative Negative Negative 19 20 21 22 23 Glucose Positive (fermentative) Positive (fermentative) Negative Negative Positive (fermentative) Negative Positive (oxidative) 24 Lactose 25 Mannitol 26 Sucrose 27 Xylose Inositol 28 29 Galactose

Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme. Test Unknown Isolate B Luminescence Cell Shape Gram Staining Oxygen Requirement Motility Drop) Positive Straight Rods Negative Facultative Anaerobe 2 3 4 (Hanging Motile Growth at Negative 0% NaCi 3% NaC1 Positive 6% NaCl Positive Accumulation of PHB Flagella Oxidase Positive 1 Polar 10 11 12 Positive Nitrate Reduction Negative Positive 13 Gelatinase Growth at 35°C Growth at 4°C Methyl Red Voges-Proskauer Catalase Starch Hydrolysis Lysine Decarboxylase Indole Production Utilization of 14 15 Negative Positive 16 17 Positive 18 Positive Negative Negative Negative 19 20 21 22 23 Glucose Positive (fermentative) Positive (fermentative) Negative Negative Positive (fermentative) Negative Positive (oxidative) 24 Lactose 25 Mannitol 26 Sucrose 27 Xylose Inositol 28 29 Galactose

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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You must write up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain and the second will be Gram negative. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes. Can you please help me find the dichotomous key . Thank you !
You must find up your OWN dichotomous key for all the possible unknown organisms listed below. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Note : The test must be performed using any of this test Mannitol Salt Agar, DNAse test, Catalase test, coagulase test, Novobiocin Test, BILE ESCULIN AGAR (BEA) TEST, Oxidase test, Bacitracin Sensitivity, Blood Agar . Macconkey, Nitrate Reduction test. Thank you so much in Advance !
You must write up your OWN dichotomous key for all the possible unknown organisms listed below . The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures . Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes Imp Note - the test should be performed using any of the following tests stated below. Bile Escalin test, Oxidaze test, Blood Agar , Catalese Test, Mannitol salt agar . Thank you !
A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/L
What are the materials and methods to achieve identification code (not the actual identification code) and the method to achieve the unknown identity since the "unknown organism" is identified using a numerical code from the bioMerieux catalog/APIweb that lists each reference species with its 7 digit code. Gram stain of the isolate to be identified, the oxidase test , the API 20E strip used and the reagents added .
Complete the table below with the observations: include amount of growth (-, +, ++, +, ++), colonial morphology for NA, color of colonies for EMB, and media color for MSA. Organism/ Media Nutrient Agar (NA) Eosin Methylene Blue (EMB) Agar Mannitol Salt Agar (MSA) Escherichia coli Pseudomonas aeruginosa Staphylococcus epidermidis Staphylococcus aureus Unknown 9 mixture
Answer the following questions briefly and concisely 1.How do bacteria in a chemostat and those in a batch culture vary from one another? 2. What happens in a chemostat if the dilution rate is higher than the organism's maximum specific growth rate? 3.Does a chemostat require the use of pure cultures? 4. Why would a complicated culture media for Leuconostoc mesenteroides be simpler to make than one with a fixed chemical composition?
Purpose Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Please help me with this question. Thank you so much !
Given the illustration and values below, determine the concentration of the original sample. Report results in CFU/mL or colony-forming unit/milliliter. Note that in order to observe the accuracy of results, culture plates with countable colonies between 25-250 CFU are considered in standard bacterial plate count.
Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________
The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1
You have used morphological observations and biochemical tests to identify two unknown bacterial isolates in your practical sessions, briefly describe three other techniques that can be used to identify unknown bacteria (you can use any reliable online resources). Note: These must not be based on biochemical tests (enzyme use, acid production etc) or morphological characteristics (size, shape, structures).