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If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to the
medium was observed?
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- Why is the looping out method preferable for sputum specimen for acid fast smears?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?One hundred microliters (0.100 ml) of a 10-5 dilution of a bacterial suspension plated on an agar plate gave rise to 229 distinct colonies. What was the concentration of bacteria in the original, undiluted suspension?
- There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectivelyWhy are semi-solid media such as TTC deeps suitable to test motility of bacteria?Why does a streaking method used to inoculate plates result in isolated colonies?
- Below are morphological, physiological and biochemical characteristics of an unknown luminous bacterium isolated from the gills of a marine fish. Use the data below to identify the isolate. Make an identification scheme Test Unknown Isolate B 1 Luminescence Positive 2 Cell Shape Straight Rods 3 Gram Staining Negative 4 Oxygen Requirement Facultative Anaerobe 5 Motility (Hanging Drop) Motile 6 Growth at 0% NaCl Negative 7 3% NaCl Positive 8 6 % NaCl Positive 9 Accumulation of PHB Positive 10 Flagella 1 Polar 11 Oxidase Positive 12 Nitrate Reduction 13 Gelatinase Negative 14 Growth at 35OC Positive 15 Growth at 4OC Negative 16 Methyl Red Positive 17 Voges-Proskauer Positive 18 Catalase Positive 19 Starch Hydrolysis Negative 20 Lysine…Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration.a) explain in your own words how to do a nitrate reduction test to identify unknown bacteria b) explain in your own words how to perform a phenol red broth (prb) test to help identify unknown bacteria
- If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic intestinal bacteria?A culture with approximately 4x105 cells/mL were incubated. After 10 hours, the number of cells had increased to 5x109. a) How long was the generation time in minutes?b) How many generations have occurred?