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how to know if my product was pure usiing a TLC plate that has reaction mixture, co spot, and starting material lanes?
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- The solvent level in the development chamber should be above the level of the spotted compounds on the TLC plate. Group of answer choices True FalseA student ran a TLC plate using a 90:10 hexane:ethyl acetate developer and this was the outcome. How can they improve their next trial?A) What are the basic principles underlying TLC technique? B) Draw a TLC plate and explain how TLC can be used to identify the presence of a specific compound in an organic mixture.
- Your HRP sample had a concentration of 2.0 mg/mL and you added 10 uL to a tube containing 10 uL of 2X Sample Buffer. You mixed this well and then heated at 75C for 5 min. Then you added 10 uL of this solution to a well of a gel. How much protein did you add to that well?A TLC plate was run in cyclohexane:ethyl acetate 4:1, the pure product, 2 starting materials and final product were spotted. what does the tlc tell you about the product.D. Solvent Extraction Samples Upper phase Lower phase Oil + Water Kerosene + Oil
- How you can improve resolution in HPLC analysisSelect one: O True O False To make a w/o emulsion you will need a surfactant with low HLB value; e.g., 6. Select one: O True O FalseA TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ B
- 5. The following TLC plate was developed in 4:1 hexane/acetone. a) Draw what the TLC plate could look like if it were developed in 9:1 hexane/acetone. b) Draw what the TLC plate could look like if it were developed in 1:1 hexane/acetone. 4:1 hexane/acetone 9:1 hexane/acetone 1:1 hexane/acetone4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.An analgesic drug sample was analyzed using TLC to determine its probable active ingredient. The compound may contain compound A, compound B, compound C, or compound D. Five spots were made on the TLC plate, of which four are for the standard compounds (A, B, C and D) and one for the test sample. The solvent was then allowed to pass through the TLC plate. The calculated Rf values of compound A, compound B, compound C, compound D, and the drug sample were 0.24, 0.61, 0.40, 0.89, 0.38, respectively. a. What could be the active ingredient in the analgesic drug sample? Why?