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- How is the information from a Kirby-Bauer disk diffusion test used for the recommendation of the clinical use of an antimicrobial drug?
- Who should be responsible for discovering and developing new antibiotics? Support your answer with reasoning.
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- Ex. 17: Chemotherapeutic Agents Using the Antimicrobial Susceptibility Table on pg 62 in the lab manual, determine whether not the bacteria plated in this Kirby-Bauer test are resistant (R), intermediate (I), or susceptible (S). The bacterial species tested was Escherichia coli (Gram negative). Ciprofloxacin Diameter = 20 Streptomycin Diameter 30 Carbenicillin Diameter 17 + Erythromycin Diameter = 23 Novobiocin Diameter = 0 bacterial growth = disk with antibiotic (see labels) = no growthHow are dilution susceptibility tests and disc diffusion tests usedto determine microbial drug sensitivity?What are the tests to determine the level of antimicrobial activity of a antimicrobial agent? How dilution susceptibility and disk diffusion test can be used to determine the effectiveness of a drug?
- The following results were obtained from a disk diffusion test for microbial susceptibility to antibiotics. Staphylococcus aureus was the test organism. Use these data to answer questions. The following results were obtained using the Kirby-Bauer method to assess microbial susceptibility to antibiotics. Staphylococcus aureus was the experimental organism. Use the data below to answer the questions. antibiotics Zone of Inhibition TO 3mm В. 7mm C Omm 10mm The most effective antibiotic was: Select one: O TO B. can't tell O O O O OBroad-spectrum drugs target a wide variety of bacterial pathogens. Even when the broad-spectrum drug is capable of killing a target pathogen, it may not be the best treatment. Which statement best explains why a broad spectrum drug might be an undesirable treatment choice? Broad-spectrum antimicrobials are usually toxic to the host cells. Broad-spectrum antimicrobials only work if they are used shortly after the infection begins. Broad-spectrum antimicrobials may kill much of the normal microbiota. Broad-spectrum antimicrobials are not able to kill bacteria that are in their log phase of growth. Broad-spectrum antimicrobials may stimulate excess growth of the normal microbiota.Why is the effectiveness of an antimicrobial agent determined using an exponentially growing bacterial culture? Please explain.
- Calculate the number of bacteria (CFU/ml) in each serum and saline sample. What was the purpose of the saline? Which bacteria produced more CFU/ml in serum? Why? Provide a possible explanation for the ability of Pseudomonas to colonize mucous membranes. Reference(s): Include at least 1 valid reference for your research in correct format (either APA or MLA) (2pts)Briefly discuss the potential impact on the antibiotic susceptibility testing results when different agar thicknesses are used across different laboratories. What would you suggest to ensure that results between testing laboratories are comparable.diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14
- The Kirby -Bauer antimicrobial sensitivity testing method relies on: A. The inhibition of one antimicrobial by another B. The diffusion of antimicrobials in paper discs C. Serial dilutions of antimicrobials in test tubes D. A gradient of an antimicrobial on a paper stripWhich one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? Select one: a. Agar macrodilution method b. Gradient diffusion method c. Broth microdilution method d. Disk diffusion methodDiscuss the procedure and application for the agar well diffusion method of evaluating antimicrobial activity.