Cystic fibrosis (CF) test to screen for mutations associated with the CFTR gene. Positive samples will undergo gene sequence analysis. What assay format is used in the screening test.
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- I understand that microarrays are being used to define the molecular abnormality and the prognosis in some patients with leukaemia. What are microarrays?Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? ExplainBriefly explain Current Infection Marker (CIM) test, Campylobacter-Like Organism (CLO) test, and the multiplex polymerase chain reaction test (PCR). Which of the tests had the highest accuracy and what methodology and techniques is used for Multiplex PCR test.
- A student performed a plaque assay and noticed that the plaques formed were very small. In a few sentences, state ONE reason why this might occur and how the student might resolve this issue.Is this DNA extraction procedure needed/necessary for COVID19 screening? If not, what is its counterpart? Describe the procedure done.A patient has a blood bank history of being type A positive. The patient has recently been diagnosed with carcinoma of the colon and now the reagent anti-B is showing a 1+ reaction with the patient's cells. Other typing results are as expected. Please explain.
- Answer the following questions. 1. Explain the steps associated with DNA profiling and state its advantages and disadvantages, 2. Describe how mutations are linked to DNA polymorphism. GUIDELINES: Each answer should be 250 words in length. Content should not be copied. References and bibliography should be provided for the content and images. please asapWrite and discuss about the analysis procedure used for carrier screening for the single-gene diseases that is routinely conducted in different countries. (Subject: Genetic engineering).are best suited for diagnosing a disorder that is caused by low protein expression due to unwanted DNA methylation. Biochemical tests Molecular tests Cytogenic tests Carrier tests Drug tests
- STR markers: are point mutations detectable by DNA sequencing are variations in the number of repeats of very short DNA motifs (2-10 nucleotides) □have high polymorphism are mutations leading to proteins or blood groups that can be differentiated by antigenic testing from a blood sample ☐have low polymorphism no correct answer are changes of a few nucleotides leading to the absence or presence of a site recognized by a restriction enzyme are variations in the number of repeats of medium-sized DNA motifs (10-100 nucleotides) can be located in coding sequences are located exclusively on autosomesGive only typing answer with explanation and conclusion Information: 1_Green Fluorescent Protein 2_nucleotide sequence, Amino acid sequence, and primers are obtained. 3_PCR protocol already described 4_bp has been calculations and estimated agarose gel image already designed. Questions: How do you analyze whether your target protein is expressed by E. coli cells. Explain your analysis method in detail and give information about the results you expect (in detail please)Provide a brief summary (3 – 5 sentences) about information of the GATA3 gene (frequency of mutations, types of mutations, details of patient data such as health symptoms expressed due to the mutated gene above , etc.).