During nucleic acid hybridization, the probe is labelled Question 1 options: for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification
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- Search (Option + Q) rences Review View Help Editing v A^ Dv A A A Question 1 During nucleic acid hybridization, the probe is labelled for DNA stability to increase probe-test DNA binding to identify the location of probe and the test DNA binding for amplification Question 2 10 IV 12 13 14 Which of the following best describes the trait in the pedigree? Question 2 options: X-linked dominant X-linked recessive autosomal domiant autosomal recessiveוןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…personal/eenongen_my_tnstate_edu/_layouts/15/doc.aspx?sourcedoc={a6b083c9-a226-4c31... O Search (Option + Q) Review View Help Editing v A BI U Ov =<=v 三 v ... Question 13 Zinc finger nuclease is a Gene editing tool: which uses a site-specific endonucleases containing a DNA-cleavage domain and a modular DNA-binding domain RNA-guided endonucleases programmable nucleases Exonuclease Question 14 Which elements are required for DNA to behave as a chromosome in yeast cells: a centromere an autonomous replicating sequence two telomeres Antibiotic selection marker Question 15 A series of such clones where the insert of each clone partially overlaps that of its neighbors with no gaps is known as a Clone Contig DNA fingerprinting A tiling path Genome MacBook Pro
- Please I want the answer of (B) and (C) Task 1. Your graduate advisor asks you to amplify the following sequence of DNA by PCR: 5’-ATACGCATTCGGACCAGGTCCTAA-3’ 3’-TATGCGTAAGCCTGGTCCAGGATT-5’ a. To ensure that the entire sequence above is amplified, what 6-nucleotide DNA primers should you add to your PCR mix? You order the primers listed above, but instead receive the following set of primers: 5’-CGCATT-3’ 5’-GGACCT-3’ b. What portion of the double stranded DNA molecule will be amplified after 10 rounds of PCR? Your labmate attempts to rescue your PCR reaction by providing you with the following set of primers: 5’-ATACGC-3’ 5’-TCCTAA-3’ c. What is the result of running the PCR reaction with your labmate’s primers? How many double stranded molecules of DNA will result from 10 rounds of amplification?15/doc.aspx?sourcedoc={dac0ebeb-b019-4578-911c-98.. Search (Option + Q) Review View Help Table O Editing er Av A =Titled “Techniques utilized to generate a genetically engineered organism and confirm gene disruption.” a. In the table, indicate with an "x" if the reagent or technique applies to DNA, RNA, and/or protein. You can have more than one "x" in each row or none at all.Search (Option + Q) Review View Help O Editing v A BIU D Av A ... Question 4 The use of physical or chemical nonviral transfer of genetic material is called Transfection Transduction Transformation Trangenesis Question 5 Congenital hearing loss is mostly autosomal recessive. When two persons of congenital hearing loss marry, as they often do, their biological children have normal hearing. The reason for the children to have normal hearing is that one of the parents is homozygous recessive for one gene and the other parent is homozygous recessive for another gene all the normal children have reverse mutations in their DNA each of the parents has reverse mutations in the germline cells early in their life. all normal children are homozygous recessive. Question 6 The enzyme responsible for transcribing complementary DNA from MRNA is DNA Polymerase Reverse transcriptase RNA Polymerase Endonucleaseacconline.austincc.edu/ultra/courses/_891351_1/cl/outline O gel electrophoresis O PCR O genomic library DNA microarray A patient is suspected of having a certain type of cancer that involves several genes. Doctors want to determine whether this patient is expressing these cancer genes and to what leve each gene is being expressed. What technique may be used? O molecular cloning O CRISPR gene editing 5 genetic recombination that occurs naturally (think horizontal gene transfer, meioșis, fertilization, or mutations). Ar : Y EV АУ ΩΘΙ 用く X A MacBook Air & 8 88 Q V Ix % 0 8A EXE D B 图 Q 用图 5 d Show All † 19 EHR ImmunizaFinal Assessment for Exploring M X forms/d/e/1FAIpQLSe8RrfvHEr59pISGjbEneL041GxKTvyw9-Xc_EA7Um_8xqReA/viewform?hr_submission-Chg1990yzQ8SEAjwvY285... First mRNA base (5) end of codon) A Sequence C Sequence D 5. A DNA sequence of "ACG" will code for the amino acid UUG CUC CUA CUG wh GUC GJA GUG Cys 100 AUU AUC lle ACC AUA ACA AUG MACG] Val M Second mRNA base UCA UCG The GCU GCC.. GCA GIGA CAA CAG Tyr UAC UGC UAA Stop UGA Stop A UAG Stop UGG Tro AAU AAC MT AAC GAC GAG 2 Lys Asp CGA CGG AGU AGC AGA AGG Lys GGU GGC GGA GGG Arg DUAU Gly C Third mRNA base (3' end of codon) Lys (K) DELL C GU A C DEOTOCCAGUCAGUCAGUCHOSOTOS M G (F) A (LS1-1) * GU A C CUGA OPCUGACU GACUSACCO AD A G OCO Trp (W) \u« 9799%3Fo 33 4Wa... SLSU SVM Status... X ↓ O 650 O 550 O 500 200 O 850 Smal ↓ O 1550 Applicant Dashbo... 300 Msel 350 Probe Smal ↓ Caption: Restriction enzyme sites are indicated by the arrows. Smal binds to CCCGGG sequences. Msel binds to TTAA sequences. Enzyme 'X' refers to an unnamed restriction endonuclease that is not affected by DNA methylation. The distance between enzyme sites is indicated as number of bases. Assume only CpG methylation occurs. A human genomic segment (depicted above) was subjected to various experimental conditions (enzyme digestion, DNA methylation) AS INDICATED BELOW. 200 If the DNA was not methylated and digested with enzymes X, and Smal, what is the largest DNA fragment size that would show up on a Southern blot using the radiolabeled probe? Smal ↓ 500 MacBook Pro X ↓ersonal use only, do not reple 2021-05-12 1ore 7. Restriction endonucleases recognize particular sequences of DNA and then cut the 16@gmail.com DNA into fragments (see table below). RESTRICTION ENDONUCLEASES BamHI DNA SEQUENCE RECOGNIZED* Luse only, do no repro 2021-05 va16@gmail.com GIG ATC C С СТАGJG GỊA A TT C СТТААЈG Pers EcoRI * Į indicates where the DNA backbone is cut The base sequences of DNA from two different individuals, Alice and Ben, are given below. Indicate where BamHI and EcoRI would cut these DNA sequences by drawing lines (for hints refer to FIGURE 2 and the video in the Canvas assignment for this section ). Personal use ofy 2021-05-12 forevermayal6@gmail.com BEN LA ALICE G C G C uCE G C Personal use only. do no G C 2021-05-12 A T Com G C G C forevermaya16@gnT A C G A T ТА C G C G C G G C G C А Т PersA T G C vermaya16@amail.com T A use only, do not reproduce. 2021-05-12 A T А Т А Т ТА ТА ТА C G 7. Does BamHI cut the DNA from Alice and Ben in the same place? C G…☆回 w Insert Format Tools Add-ons Help Last edit was yesterday at 8:08 AM TURN 100% Normal text Arial 11 BIUA E- 1E E E - E EX 2. 3 . 5. C Directions: Describe the process of DNA replication in the space below. Specifically reference the pictures in your answer. Your response will undergo a plagiarism check. Be sure to write your response in your own words. Be sure to reference and thoroughly explain: The relationship and importance of DNA replication and the cell cycle. The conservation of genetic information during replication. (Is it conservative? Semi-conservation? Dispersive? What does this mean?) The specific enzymes used in each step and indicate whether each of the enzymes is a synthesis reaction or a digestion reaction. Justify your answer through explanation. Identify the parameters and limitations of the enzymes working effectively. (What factors would limit or interfere with DNA replication?)SEE MORE QUESTIONS