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- Briefly describe the rationale and method of color compensation in flow cytometry.
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- A flow cytometer is constructed of many components. For good quantitative results how and how frequently should the various systems be calibrated? What is MESF and how does it apply in flow cytometry?Define reversibly arresting cells by flow cytometryProvide two advantages and two disadvantage of flow cytometry analysis. Briefly explain each.
- Draw the different types of WBC and state each function. What is the principle behind flow cytometry What is/are purpose of flow cytometryWhat kind of cells are suitable for flow cytometric analysis and why? Please answer with 5 dot points/sentences minimumPrecipitin test is typically performed to identify the origin of blood. What are the advantages of using the gel diffusion method over the others (capillary method and electrophoretic method)?
- Briefly explain the technique for preparation and interpretation of disc diffusion tests.The following questions refer to the analysis of cells using flow cytometry A) When analyzing cells in a flow cytometer how do you ensure you are analyzing single cells rather than doublets or clusters of cells and why is that important? B) How do forward versus side scatter plots enable the characterization of different types of blood cells in the blood of a patient? How does this relate to a potential diagnosis of acute leukemia? C) How do you determine if a patient has B cell versus T cell Leukemia? Why is it important to know?Discuss the principle and step by step procedure of Dry Blood Spots collection.?
- Summarize the steps for general Hematoxylin and Eosin staining procedure through a flow chart. kindly make use of a flowchart for better understanding, and please focus on the general Hematoxylin and Eosin staining procedureWhy mixture of solvents such as ethyl acetate with 5% acetic acid is used as a solvent/mobile phase for developing TLC?Describe what the reverse pipetting technique consists of and in what cases it is used.use in the laboratory