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- For an exponentially growing culture that increases from 5 *106cells/ml to 5 * 108cells/ml in 8 h, calculate g, n, and k forthis culture.A culture consisting of 100 litres of nutrient medium containing 12 g l-1 of growth limiting substrate is inoculated with 10g of bacteria in a batch fermenter. The yield of biomass from substrate is 0.05 g g-1 . The maintenance coefficient is 2.0 g g-1 h -1 . The specific rate of product formation due to maintenance is 1.0 h-1 . The maximum specific growth rate of the organism is 0.3 h -1 . YPX= 7.7 and product formation is directly linked with metabolism. a) What is the time required to produce 15 g of biomass? !!!!! ANS IS 3.07 HRS!!!! STEPS on how to reach this pleaseE. coli has a doubling time of 0.345 hours at 37 0C. Starting with 1 milligram of cells, assuming a lag phase of 30 minutes and a stationary phase of 30 minutes, what number of cells would you expect at the end of 13h, assuming that besides the lag and stationary phases the culture is continuously in exponential phase. Assume that ln (2) = 0.69 and that the mass of a single cell is 10^(-12) g.
- Yeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. = 1.3 g cells / L. The growth mediğim in the reactor, which is well-mixed so the cells have access to all nutrients, contains 150 g CELL DATA ribose (C5H10O5, MW 150), 75 g ammonia (NH3, MW 17), and 85 g oxygen (O2, MW 32). A-10. Determine the maximum concentration of cells, [X], that will form in the bioreactor when the limiting nutrient is consumed. Search Yx/ribose YX/02 YX/NH3 Specific growth rate on limiting nutrient, u Lag phase duration Deceleration phase duration 0.48 g/g 0.87 g/g 0.23 g/g 0.51 h 30 minutes Negligible(b) A Food material containing Bacillus stearothermophilus PS1518 as an indicator organism ts subjected to heat sterilization at 121 C. Calculate the time required to reduce the organism to one tenth of the original number. Fo value for the organism is 4 minutes and the decimal reduction time , D, at 116°C is 40 minutes. Assume operation is at constant temperature of 121°C. HINT Fo = -To 10 dt Where Fo = equivalent exposure time at 121°C of the actual exposure time at a variable temperature To = the reference temperature =121 C, z=10 = number ofC necessary for10fold increase in F8. A. Use Excel (or another graphing program) to draw the growth curve, In (X/X.) vs time, for bacteria grown in a 20 L suspension cell culture, given the following data: - initial concentration: 0.120 gdw cells/L Also report: - lag time: 1.5 hours - mass doubling time during exponential growth: 250 minutes - duration of exponential growth phase: 1 day (24 hours) - negligible time in the deceleration phase - 13 hours in the endogenous metabolism phase with no change in cell concentration - cell death rate with k = 0.0178 min -¹. B. What is the specific growth rate, µ? C. What is the maximum concentration of cells in the reactor? (gdw cells/L) and when does this occur? D. Other than time zero or the end of lag phase, at what time is the concentration of living cells in the reactor equal to the initial concentration of 0.120 gdw/L?
- Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium: (1) culture A – cells are in lag phase; (2) culture B – cell in log phase; (3) culture C – cells in stationary phase; and culture D – cells in decline phase. Imagine that you observe the growth rates of each culture in a fresh sterilized LB medium. Now, plot the outcome growth curves of cultures A, B, C and D in a single graph.
- Sydney Brenner isolated Salmonella typhimurium mutants that were implicated in the biosynthesis of tryptophan and would not grow on minimal medium. When these bacterial mutants were tested on minimal medium to which one of four compounds (indole glycerol phosphate, indole, anthranilic acid, and tryptophan) had been added, the growth responses shown in the following table were obtained. Mutant Minimal medium Anthranilic Indole glycerol acid Indole Tryptoph phosphate trp-1 trp-2 trp-3 trp-4 trp-6 trp-7 trp-8 trp-9 trp-10 trp-11 - Give the order of indole glycerol phosphate, indole, anthranilic acid, and tryptophan in a biochemical pathway leading to the synthesis of tryptophan. Indicate which step in the pathway is affected by each of the mutations. + 1 + 1 IIThe data below were obtained for the growth of a pure culture of Escherichia coli in nutrient broth at a temperature of 37°C. Determine (1) the specific growth rate and (2) generation time of E. coli and the duration of the (3) lag and (4) log (or exponential) phases. (ln 2 = 0.693) Time (h) 0 1 2 3 4 8 16 32 Bacterial No./mL 104.1 103.9 104.4 105.5 106.5 107.7 108.0 107.6Consider the desired phenotype pattern of candidates that we wish to advance into experiments in Week 5 and beyond. If the growth patterns illustrated below are observed in your Week 4 results, which of the candidates would you select for further analysis? Select all that apply. Candidate A B A B C с = vigorous growth on this medium = impaired/slow growth on this medium = no growth on this medium SC-H B SC-K A B YP Gal