B-lactamase is an enzyme found in many antibiotic-resistant bacteria that hydrolyzes and inactivates antibiotics like penicillin and cephalosporin. The amount of antibiotic hydrolyzed in 1 minute in a 10-ml solution containing purified ß-lactamase was measured as a function of antibiotic concentration. The kinetics of hydrolysis was performed for two antibiotic substrates (A and B). Assume that the concentration of ß-lactamase was kept constant during the assay. 12 10 00 Initial Velocity (nanomole/min) N 0 10 T 20 H 30 [Antibiotic] (µm) T 40 I 50 Antibiotic A Antibiotic B a) Based on the enzyme description, what type of enzyme is ß-lactamase? Lyase Isomerase Ligase Hydrolase Oxidoreductase Transferase b) Based on your answer in (a), what other reactant, in addition to the antibiotic substrate, needs to be in the active site of ß-lactamase for the hydrolysis reaction to proceed? c) From the reaction curves above, what is the approximate value of Vmax for the enzyme reaction? (Do not forget the units!) With Antibiotic A: With Antibiotic B: d) From the reaction curves above, what is the estimated KM of the enzyme? (Do not forget the units!) For Antibiotic A: For Antibiotic B: e) Which binds more tightly to ß-lactamase Antibiotic A or Antibiotic B? Explain your answer.
B-lactamase is an enzyme found in many antibiotic-resistant bacteria that hydrolyzes and inactivates antibiotics like penicillin and cephalosporin. The amount of antibiotic hydrolyzed in 1 minute in a 10-ml solution containing purified ß-lactamase was measured as a function of antibiotic concentration. The kinetics of hydrolysis was performed for two antibiotic substrates (A and B). Assume that the concentration of ß-lactamase was kept constant during the assay. 12 10 00 Initial Velocity (nanomole/min) N 0 10 T 20 H 30 [Antibiotic] (µm) T 40 I 50 Antibiotic A Antibiotic B a) Based on the enzyme description, what type of enzyme is ß-lactamase? Lyase Isomerase Ligase Hydrolase Oxidoreductase Transferase b) Based on your answer in (a), what other reactant, in addition to the antibiotic substrate, needs to be in the active site of ß-lactamase for the hydrolysis reaction to proceed? c) From the reaction curves above, what is the approximate value of Vmax for the enzyme reaction? (Do not forget the units!) With Antibiotic A: With Antibiotic B: d) From the reaction curves above, what is the estimated KM of the enzyme? (Do not forget the units!) For Antibiotic A: For Antibiotic B: e) Which binds more tightly to ß-lactamase Antibiotic A or Antibiotic B? Explain your answer.
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter10: From Proteins To Phenotypes
Section: Chapter Questions
Problem 10QP: b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein...
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![B-lactamase is an enzyme found in many antibiotic-resistant bacteria that hydrolyzes and
inactivates antibiotics like penicillin and cephalosporin. The amount of antibiotic hydrolyzed in 1 minute
in a 10-ml solution containing purified ß-lactamase was measured as a function of antibiotic
concentration. The kinetics of hydrolysis was performed for two antibiotic substrates (A and B). Assume
that the concentration of ß-lactamase was kept constant during the assay.
12
Initial Velocity (nanomole/min)
10
8
2
0
10
20
|
30
[Antibiotic] (µm)
40
50
Antibiotic A
Antibiotic B
a) Based on the enzyme description, what type of enzyme is ß-lactamase?
Lyase
Isomerase
Ligase Hydrolase
Oxidoreductase
Transferase
b) Based on your answer in (a), what other reactant, in addition to the antibiotic substrate, needs to be in
the active site of ß-lactamase for the hydrolysis reaction to proceed?
c) From the reaction curves above, what is the approximate value of Vmax for the enzyme reaction? (Do
not forget the units!)
With Antibiotic A:
With Antibiotic B:
d) From the reaction curves above, what is the estimated K of the enzyme? (Do not forget the units!)
For Antibiotic A:
For Antibiotic B:
e) Which binds more tightly to ß-lactamase Antibiotic A or Antibiotic B? Explain your answer.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa4cfac85-c063-4626-8a51-e81b784ec2bd%2F54cdb8d1-b9c0-4701-a364-2ae56b9ee372%2F8dbv9w_processed.png&w=3840&q=75)
Transcribed Image Text:B-lactamase is an enzyme found in many antibiotic-resistant bacteria that hydrolyzes and
inactivates antibiotics like penicillin and cephalosporin. The amount of antibiotic hydrolyzed in 1 minute
in a 10-ml solution containing purified ß-lactamase was measured as a function of antibiotic
concentration. The kinetics of hydrolysis was performed for two antibiotic substrates (A and B). Assume
that the concentration of ß-lactamase was kept constant during the assay.
12
Initial Velocity (nanomole/min)
10
8
2
0
10
20
|
30
[Antibiotic] (µm)
40
50
Antibiotic A
Antibiotic B
a) Based on the enzyme description, what type of enzyme is ß-lactamase?
Lyase
Isomerase
Ligase Hydrolase
Oxidoreductase
Transferase
b) Based on your answer in (a), what other reactant, in addition to the antibiotic substrate, needs to be in
the active site of ß-lactamase for the hydrolysis reaction to proceed?
c) From the reaction curves above, what is the approximate value of Vmax for the enzyme reaction? (Do
not forget the units!)
With Antibiotic A:
With Antibiotic B:
d) From the reaction curves above, what is the estimated K of the enzyme? (Do not forget the units!)
For Antibiotic A:
For Antibiotic B:
e) Which binds more tightly to ß-lactamase Antibiotic A or Antibiotic B? Explain your answer.
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