ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 µM ATP (1000 μL of each) Inhibitors Rotenone Oligomycin CCCP KCN Antimycin A Calculate the needed volumen of the standards! Inhibitors of ETC Stock concentations Final concentrations (in 250 μL) 400 µg/ml in DMSO 1250 µg/ml in EtOH 1000 µg/ml in DMSO 400 mM in Tris-buffer 1 mg/ml in DMSO Calculate the needed volumen! 8 µg/ml 25 µg/ml 20 µg/ml 8 mM 20 µg/ml
ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 µM ATP (1000 μL of each) Inhibitors Rotenone Oligomycin CCCP KCN Antimycin A Calculate the needed volumen of the standards! Inhibitors of ETC Stock concentations Final concentrations (in 250 μL) 400 µg/ml in DMSO 1250 µg/ml in EtOH 1000 µg/ml in DMSO 400 mM in Tris-buffer 1 mg/ml in DMSO Calculate the needed volumen! 8 µg/ml 25 µg/ml 20 µg/ml 8 mM 20 µg/ml
Chapter13: Dimensional Analysis/units Conversion
Section: Chapter Questions
Problem 2.4P
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Transcribed Image Text:CALCULATIONS OF THE
SOLUTIONS FOR EXPERIMENT
Substrates
Stock concentations
Succinate + glutamatate (G/S) 150 mM Glutamtate + 150 mM Succinate
Malate + pyruvate (M/P)
30 mM Malate + 30 mM Pyruvate
Inhibitors
Substrates of ETC
ATP stock (50 µM): Make pre-dilutions with 20, 15, 10, 5, 2,5 and 1,25 μM
ATP (1000 μL of each)
Rotenone
Oligomycin
CCCP
KCN
Antimycin A
Calculate the needed volumen of the standards!
Inhibitors of ETC
Stock concentations
400 µg/ml in DMSO
1250 µg/ml in EtOH
1000 µg/ml in DMSO
400 mM in Tris-buffer
1 mg/ml in DMSO
Calculate the needed volumen!
Final concentrations
(in 250 μL)
8 μg/ml
25 µg/ml
20 µg/ml
8 mM
20 µg/ml
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