Table 1. Different Staining Techniques Gram Stain Acid Fast Endospore Capsule Flagella 1. Principle 2. Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature's) 3. Stain's Used 4. Type of Stain 5. Mechanism of Staining (of each stain used) 6. Flow of Method
Q: Which of the following cell types will most likely reach cell senescence after many rounds of replic...
A: Introduction: Senescence is the gradual deterioration of functional characteristics with age.
Q: After irradiating wild-type cells of Neurospora (a haploid fungus), a geneticist finds two leucine-r...
A: Part A. In the given question, the different types of alleles that are mutant in nature are brought ...
Q: One of the major phylogenetic conclusions of this study is: choose one a. The Ginkgoales ar...
A: Answer : d. The Gnetales are the sister group to angiosperms among extant gymnosperms.
Q: Consider the following F, individuals in different snapdragon species and the F2 ratios produced by ...
A: "Genetics" is the study of the functioning and main codes of variation and heredity. Inheritance is ...
Q: answer the question. The bombardier beetle is spraying a boiling hot liquid that contains irritating...
A: Introduction: Beetle is considered to be the gardener's friend and it is a fearsome predator as it c...
Q: Enumerate and describe the patterns of cleavage exhibited by the frog.
A: Cleavage is a process that occurs in early embryogenesis, whereby a single fertilized egg cell (the ...
Q: How do these work? i. Artificial selection ii. Natural Selection iii. Gene flow iv. Non-random matin...
A: Introduction :- Selective breeding is the process of humans using animal and plant breeding to selec...
Q: When comparing evolutionary similarities between different genes within a gene family, it is usually...
A: DNA is a basic hereditary molecule that carries information related to the person.
Q: a) Provide a description of the the small intestine epithelial cells observed under high power objec...
A: Histopathology:- Small Intestine Mucosa: - Type of Lining: - Simple Columnar Epithelium Morphology:-...
Q: Where do spindle fibers attach on a chromosome? O cell plate O meristem centromere
A: The chromosomes can be described as the thread like structures that can be found in the nucleus of t...
Q: An il or injured patient may have suffered from damaged tissues that the cell cycle, including mitos...
A: Cloning is the procedure of creating a new multicellular organism, genetically identical to another,...
Q: Pt the events of action potertl generstion in the onder tut they occur from fst ) to last (S wved ot...
A: * Action potential is a when s cell was activated by stimulus reversal in electrical potential acro...
Q: Which of the following is NOT one of the roles of algae in nature? O a. Petroleum is the fossil rema...
A: Introduction : Features of algae : Body is relatively simple unicellular or multicellular thallus ...
Q: Ear Drum Cochlea Oval window (i) Give the biological term for the part labelled 'A' and state its fu...
A: Ear is one of sensory organ and it helps in hearing, postural equilibrium and head-eye movement coor...
Q: 1. define inheritance, genes, alleles, and other basic terms used in genetics. 2. execute the basic...
A: It was during the mid-nineteenth century that headway was made in the understanding of inheritance. ...
Q: What is chimera in recombinant dna How we can make chimeric gene Is chimeric gene is related to gmo ...
A: Recombinant DNA (rDNA) is a technique for copy and pasting DNA sequences of interest using enzymes. ...
Q: If you are a strong taster, how many bands would you expect on the gel after electrophroesis? O1 O2 ...
A: Strong taster is the person who gets the taste of food stronger than average people they are called ...
Q: In the Watson-Crick model of DNA structure: Question 5 options: T can form three hydrogen bond...
A: Introduction: DNA stands for 'deoxyribonucleic acid' and it is the hereditary material in humans and...
Q: describe why fehling's test is not an accurate for blood-glucose levels?
A: Fehling's solution is a substance reagent used to separate between water-solvent carb and ketone use...
Q: When comparing evolutionary similarities between different genes within a gene family, it is usually...
A: A gene is a sequence of DNA that codes for a specific protein. Proteins are the building blocks of o...
Q: Skeletal muscles move as coordinated by the nervous system. sympathetic somatic O peripheral O auton...
A: Skeletal muscles/Voluntary muscles Transverse lines are found at regular intervals. Hence these mus...
Q: What can be concluded about the influence of climate change on extreme weather events? a. climat...
A: Correct option is b b. climate change is intensifying many extreme weather events Erosion, loss of o...
Q: Compare the developmental stages (cleavage, blastula, gastrula) of the frog with an invertebrate pre...
A: INTRODUCTION Different stages of development of frog and sea urchin is given below.
Q: . Which of the following has a genotype with homozygous purple flower and heterozygous tall plant. C...
A: * Homozygous and heterozygous are used to describe the allele pairs. *Individuals that carry two ide...
Q: What factor could have been responsible for the changes in the population's age structure? Use the s...
A: The population age structure of a country or region is the distribution of its people's ages. This c...
Q: Provide a description of the plant cell of plastids in leaf of Hydrilla observed under high power ob...
A: Hydrilla is a hardy, fast-growing perennial with long, slender stems that can reach a length of 7 me...
Q: GIVE THE EMBRYOLOGICAL EXPLANATION FOR "The dorsal mesocardium is a ventral mesentery." please be ...
A: 1 : a ventral mesentery of the undeveloped stomach that endures as the falciform tendon and the less...
Q: How does natural selection lead to the formation of new species?
A: *Natural selection is a process of evolution. *Organisms adapted to their environment are likely to ...
Q: B: Lambda DNA/Hindlll Marker, 2 GeneRuler" 1 kb DNA Ladder O'GeneRuler" 1 kb DNA Ladder, ready-to-us...
A:
Q: In your own understanding, Indentify the hurdles that may help to lengthen the shelf-life of squash ...
A: The shelf- life of a product depends upon the condition in which the product is being placed. Better...
Q: Can someone explain this? When you cross female flies with white eyes to male flies with red eyes, y...
A: Cross occurs between white eyes females XwXw x red eyes males XWY. Red is a dominant character over ...
Q: What are the main types of waste?
A: Introduction Organic waste, recyclable waste, non-recyclable waste, toxic waste, nuclear toxic waste...
Q: 3. What's the difference between chronological age and biological age?
A: Introduction The length of time that someone or anything has lived or existed is referred to as thei...
Q: Tell how to make a 1 M solution of Mg3(PO,), and a 0.1 M solution of caso,. Ca.SO4.
A: The term molarity can be used to describe the solute concentration in a particular solution. The for...
Q: A coat color of a horse is governed by many different gene pairs. Dominant B will give the horse a b...
A: Given that, B is the dominant gene which is responsible for black color and b is the recessive one w...
Q: 13 10 13, ATLAS (caudal view) AXIS (left side) 16 CERVICAL (caudal view)
A: * Turtles reptiles also known as Testudines. *A shell is formed from their ribs. *turtles are divide...
Q: On a fox ranch in Wisconsin, a mutation arose that gavea “platinum” coat color. The platinum color p...
A: A gene is the essential physical and functional unit of heredity. They are comprised of DNA (deoxyri...
Q: Why is evolution important in explaining the diversity of life
A:
Q: The efficiency (in terms of increased outputs from fewer inputs) of modern industrial agriculture is...
A: With evergrowing population and constant land and other resources, the production per hectare needs ...
Q: Aside from Dengue Fever what other diseases can Aedes aegypti carry
A:
Q: Mouse Cat Baboon Cost to purchase one animal $5.00 $50.00 $500.00 Cost to maintain one animal for on...
A: Mouse Cat Baboon Cost to purchase $5.00 $50.00 $500.00 Cost to maintain $45.00 $200.00 $500.00...
Q: You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the al...
A: Gram negative bacteria: these bacteria are enclosed in hard capsule and don't retain the crystal vio...
Q: NAD+ b. Pyruvate
A: Fermentation is a well stated to been supposed to define as is a metabolic process that involves the...
Q: Muscle fatigue has been suggested as one factor that can increase the chances of non-contact sports ...
A: * Anterior cruciate ligaments found inside knee joint.which cross each other to form an "X" shaped...
Q: a. Explain your understanding of the renal system
A: The excretory system is a system that is found in the human body. This system's job is to eliminate ...
Q: Why have we chosen a human Rab protein with no direct yeast equivalent as an “outgroup” for this stu...
A: Rab protein: it plays important role in recruitment of organelle specific vesicles tethering factors...
Q: Hello, good day. I have a problem answering this question, and I need your help. Hoping for a respon...
A: Flies belong to the order Diptera, which has evolved advanced mechanosensory organs known as haltere...
Q: How does crossing over shuffle alleles?
A: The meiosis is a cell division process that involves division of a diploid (2n) mother cell and prod...
Q: Research have stated that there are factors that could affect the efficacy and duration of mosquito ...
A: *Mosquitoes are vectors for mamy diseases like malaria and chikungunya abd Zika virus and yellow fev...
Trending now
This is a popular solution!
Step by step
Solved in 5 steps with 5 images
- Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. Gram positive Gram negative 2. Mordant was skipped Gram positive Gram negative 3. Decolorizing step was omitted Gram positive Gram negative 4. counterstain was not used? Gram positive Gram negative 5. Crystal violet and safranin was swapped in position. Gram positive Gram negativeWhy is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 X
- Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please explain, thank you.procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slantprocedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.
- indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific…Negative staining is useful for OA.motility purposes OB.visualizing morphology OC.flagella detection OD.identifying the capsuleWrite the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates. Note: This will perform the students so please write it in the easiest manner. Thanks
- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedOut of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase TestIdentification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus) CAMP Test Coagulase Tube Test PYR Test Novobiocin Sensitivity Test