Q: What is the magnification of each of the following objectives? scanning: low power: high…
A: Microscope can produce the larger images of an object in contrast to its original size and this…
Q: Which one of the following methods of antimicrobial susceptibility testing requires a dilution…
A: Antimicrobial susceptibility testing is a method which is used for determination of antimicrobials…
Q: Considering microbial identification methods, to which category would the following method belong…
A: Microbial identification plays very crusial role in studying the growth of microorganism, with…
Q: Which of the following antimicrobial control methods would likely not beused in the clinical setting…
A: Sterilization alludes to any interaction that eliminates, murders, or deactivates all types of life…
Q: In product testing, microbial growth is found.| Interpretation: Rationale:
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Name 4 different sterilization/disinfecting methods, how they fight microbes,
A: STERILIZATION : It is the process in which removal of all the living forms (microorganisms) and…
Q: Compare and contrast antisepsis, disinfection, sanitization, and sterilization with regard to…
A: Microbes are the microscopic organisms that grow on different objects and surfaces in the…
Q: Match the description to the correct medium This medium is enrichment for fastidious [Choose ]…
A: A culture media or a growth media is a liquid, solid or semi-solid medium, which is designed to…
Q: Read the following statements. Fill-in the possible effect of the procedure and give the rationale.…
A: Microbes, which are tiny and nearly invisible, have had a huge influence on society since the…
Q: 5. Over-dilution with sterile water. Effect: Rationale:
A: Microbial suspension is a diluted form of a bacterial strain in liquid medium which contains the…
Q: a) Microbial products can be classified in three major categories which related to the microorganism…
A: Microbial metabolism defines all the internal bio-chemical pathways, through which microbes obtain…
Q: Match the description to the correct medium This medium is enrichment for fastidious [Choose ]…
A: It is important to grow microorganisms outside the body for the study of their characteristics and…
Q: procedure and application for the agar well diffusion method of evaluating antimicrobial activity.
A: Bacteria have cell walls made up of polysaccharides, that give them strength and rigidity. Bacteria…
Q: Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been…
A: steam sterilization is a method of moist heat sterilization that uses high-pressure steam to…
Q: Correlate antisepsis, sanitization, disinfection, and sterilization with agent effectiveness
A: A microorganism or organism is a creature which is minute, which implies so little that individuals…
Q: Laboratory Scenario Analysis: Read the following statements. Fill-in the possible effect of the…
A: Antimicrobial activity can be defined as all experimental process that is done to inhibit the growth…
Q: 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3.…
A: Germ free or microbial free Environment is needed to make surrounding harmless to living organisms…
Q: Answer the following: which antimicrobial would be suitable for the control of the given bacterial…
A: Bacterial culture control means to inhibit the growth of that bacteria. Growth of bacteria can be…
Q: Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri…
A: Following aseptic techniques is an important part while performing an experiment. Aseptic technique…
Q: Assigned chemical agent: How does this agent specifically destroy microbial cells? Propose a…
A: There are few important points that should kept in mind : The bacteria treated with allows stain to…
Q: Bacterial cells can form colonies on the following media EXCEPT: Select one: Nutrient broth Agar…
A: The nutrient broth is the liquid nutrient media that lacks the solidifying agent. It is used for…
Q: What appropriate method should be used in controlling microbial growth in each of the following? 1.…
A: The control of microbes and other biological agents from organic substances is called sterilization.…
Q: Agar was not completely set prior to inoculating with the organism. Effect: Rationale:
A: Bacterial cultivation requires specific types of medium that can provide some of the basic…
Q: When testing antimicrobial compounds on lawns of plated bacteria, the strongest germicide could…
A: Bacteria are prokaryotic unicellular organisms that are omnipresent in the environment. There are…
Q: Considering microbial identification methods, to which category would the following method belong…
A: There are different types of microbial identification methods, Immunological-Serological analysis,…
Q: 2. Molten agar mixed with bacterial suspension has a temperature higher than 45˚C Effect:…
A: Bacterial culture requires nutrition for growth in any environment be it in vivo or in vitro. Thus,…
Q: Distinguish between the term’s sterilization, disinfection, antiseptics, and decontamination.…
A: These all are the method of killing or reducing the number pathogenic microorganism but these terms…
Q: List one method of disinfection, and one method of sterilization. Label which is which.
A: Disinfection is a process by which most of the harmful microbes are killed or inactivated. However…
Q: Features of continuous cell culture include the following, except ________ . a.addition of product…
A: Introduction: A single cell type that may be serially multiplied in culture for a set number of cell…
Q: Differentiate therapeutic sterilization from direct sterilization What are some of the…
A: Sterilization is a medical procedure where the reproductive ability of an individual is taken off.…
Q: microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures…
A: Growth of bacteria differs with varying temperature. Bacteria grows rapidly in the temperature range…
Q: Vivid outline and Standard operating procedure of Toxin neutralization test.
A: Antigens are defined as the substances or the molecules that are present on the surface of the…
Q: The thermal death time in an autoclave at 121oC for a microbial culture was thirteen minutes. The…
A: sterilization is the process by which microorganisms in a object or surface is completely destroyed…
Q: Z-germicSide is an antimicrobial agent. Based on the given data set below, describe how effective…
A: Phenol is used as an antiseptic and disinfectant against various types of micro-organisms like…
Q: When a disinfectant or antiseptic's effectiveness is expressed as a phenol coefficient, a number…
A: Phenol coefficient: It is a way to compare bactericidal (killing bacteria) nature of a chemical…
Q: Explain the concept of and apply the aseptic technique
A: Bacteria, viruses, and fungi are the microorganism that is present everywhere. Some of them cause…
Q: LZ AMC VA CTR
A: KIRBY BAUER METHOD- The antibiotic sensitivity test is performed by using the agar diffusion method…
Q: The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii.…
A: Sterilization: Microorganisms cause contamination, infection, decay and it is important to remove…
Q: Which one of the following methods of antimicrobial susceptibility testing combines some of the…
A: Antimicrobial susceptibility tests are performed to identify which drugs are effective against a…
Q: Describe the process of qualitative water test for coliform contamination. If shown EMB plates, be…
A: * Coliform bacteria are Bacteria and stains Gram negative and they can be motile or non motile…
Q: Should you sample an area for microbial growth before or after sanitization? Eplain why.
A: Sanitization is basically the instrumental process in which the microbes are usually killed by…
Q: Identification of an organism does not require a pure culture because Bergey’s Manual will sort out…
A: Taxonomy is the branch of biology, which deals with the science of naming, defining and classifying…
Q: equipments can be used to determine turbidity in the sample tubes in testing of antimicrobial act
A: The clarity of a liquid can be measured by its turbidity. We can define turbidity as the measure of…
Q: Answer the following: given bacterial cultures? Escherichia coli MR-Staphylococcus aureus:…
A: Antimicrobial drugs are substances that both inhibit and kill microorganisms. They are drugs that…
Q: Considering microbial identification methods, to which category would the following method belong…
A: Introductin The existence of catalase, an enzyme that catalyses the release of oxygen from…
Q: Discuss the four most common nonfermentative, gram-negative bacilli isolated in the clinical…
A: Gram-negative bacteria cause infections including bloodstream infection, pneumonia, surgical site…
Q: What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
A: Bacterial culture media is a kind of growth media in which the required bacteria is cultured. The…
Q: List 2 non-chemical means of disinfection.
A: Disinfection refers to the process by which most pathogenic disease-causing microorganisms, except…
Topic: Preparation of Microbial Suspension
A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included.
Effect:
Rationale:
Step by step
Solved in 2 steps
- Topic: Spread-Plate Method Agar was not completely set prior to inoculating with the organism. Effect:Rationale:Topic: Preparation of Microbial Suspension 4. Inaccurate volume measurements of aliquots during dilution. Effect: Rationale:Topic: Preparation of Microbial Suspension 5. Over-dilution with sterile water. Effect: Rationale:
- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiTopic: Pour-Plate Method 2. Molten agar mixed with bacterial suspension has a temperature higher than 45˚CEffect: Rationale:Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient Agar
- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:Identification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus) CAMP Test Coagulase Tube Test PYR Test Novobiocin Sensitivity TestNOTE: Kindly provide references. 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and gamma-hemolytic reactions on blood agar media (BAM). Cite two (2) examples of bacteria causing the above hemolytic reactions.