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- Answer the following questions: 1. Define a bacterial colony. 2. What is the difference between macroscopic and microscopic appearance of bacteria? 3. State the three standard terms used to describe single colonies on agar plates. 4. State and define the three types of growth that may be seen in a broth culture. 5. State three basic shapes of bacteria. 6. State and describe the different arrangements of cocci. 7. What is the difference between true motility and Brownian motion?Identification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't grow well in 6.5% NaCl medium. Which test would you expect to give you a presumptive identification? (i.e. The first tests tell you the genus, which test would identify a species of that genus) CAMP Test Coagulase Tube Test PYR Test Novobiocin Sensitivity TestFrom a contaminated intravenous catheter you isolate Gram-positive cocci, beta-hemolytic, and catalase positive. MSA results below (grown aerobically): Which pathogen from the list below have you isolated? O Streptococcus pyogenes_ O Staphylococcus aureus_ Staphylococcus epidermidis_ Streptococcus pneumoniae_
- Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient AgarA culture of Staphylococcus of unknown density is diluted as follows (letters represent each step): • 60 mL of a bacterial culture is diluted by adding 440 mL of water • 1000 microliters from (A) is added to 9 mL of water • Dilution (B) is used to prepare a 103 dilution in water • 100 ul from dilution (C) is plated to nutrient agar and incubated at 37°C Following incubation, colonies are uniformly distributed on the agar, and 102 colonies are counted on one half of the plate. 1. Express the total CFU/mL of the original culture in scientific notation. 2. If 10 ul is plated from dilution (C), how many colonies should grow on the plate? Edit View Insert Format Tools TableMatch the expected result (purple, red, or colorless) to the following descriptions of Gram-stained cells. Choices may be used more than once --------Staphylococcus aureus before the primary stain
- A patient with AIDS is hospitalized with symptoms of high fever and rigidity of the neck. Routine laboratory tests on the CSF show a WBC count of 100/m L with a predominance of lymphocytes and monocytes, glucose of 55 mg/dL (plasma: 85 mg/dL), and a protein of 70 mg/dL. The Gram stain shows a questionable starburst pattern. Questions: a. What additional microscopic examination should beperformed?b. If the test is positive, what is the patient’s diagnosis?c. If the results of the test are questionable, what additionaltesting can be performedDescribe the microscopic morphology of each sample observed with a Gram stain (S. aureus, P. aeruginosa, B. subtilis and dental plaque). Indicate the Gram reaction of each.discuss the clinical significance, principles, reagents (if any) and positive and negative results of the following laboratory tests for Gram positive cocci organisms: Bile esculin test 5% salt turbidity or tolerance test PYRase test Pyruvate broth test
- This is a catalase-positive, coagulase-negative, gram-positive coccus isolated from a urine specimen from a 20-year-old female college student. The image shown is a Mueller Hinton plate streaked with a 0.5 MacFarland standardized inoculum and a 5 microgram disk of novobiocin after overnight incubation. What is the identification of the isolate? Please select the single best answer Staphylococcus epidermidis Staphylococcus aureus Staphylococcus saprophyticus Staphylococcus lugdenensisBased on the pictures provided, tell me what I have for the gram-positive, and what I have for the gram-negative? Possible Unknown Strains: Staphylococcus epidermidis, Bacillus subtilis, Staphylococcus aureus, Bacillus megaterium, Eschericia coli, Pseudomonas aeruginosa Name one negative and one positive of which I have.Kousei works at a company that produces sweetened condensed milk. If one or more samples exceed 104 bacterial CFU/mL (or CFU/g) the entire lot produced will be rejected. Kousei's boss, Kaori, wanted to know if the lot passed the FDA guidelines. The following bacterial counts were obtained after serial dilution and pour plating of the samples: Type of Dilutions plated 10-4 10-³ 10'5 Colony Plate 1 Plate 2 Plate 1 Plate 2 Plate 3 Plate 1 Plate 2 Plate 3 Plate 3 244 Bacteria 288 251 99 81 75 24 21 15 Based on the bacterial counts obtained, will the lot be rejected? Yes, because the computed CFU/mL is 2.5 x 10^7 OYes, because the computed CFU/mL is 3.0 x 10^6 No, because the computed CFU/mL is 3.0 x 10^3 No, because the computed CFU/mL is 2.5 x 10^3 OYes, because the computed CFU/mL is 3.8 x 10^5 O No, because the computed CFU/mL is 3.0 x 10^3 O Information is lacking so CFU/mL cannot be computed