23. The drug UTSAone was investigated as a possible inhibitor of a dehydrogenase enzyme that acts on pregnenolone as a substrate in steroid synthesis. The activity of the dehydrogenase was measured in the presence and in the absence of 10 µM UTSAone. Use the data in the table to construct a Lineweaver-Burk plot. [Pregnenolone, µM] Vo without I (pmol/min) Vo with I (pmol/min) 1.0 0.00106 0.00079 5.0 0.00327 0.00242 10.0 0.00439 0.00326 20.0 0.00529 0.00395 Which of the following statements are TRUE? A. The Km (uM) in the absence of UTSAone is 2.4. B. The Km (µM) in the absence of UTSAone is 5.4 The Km (µM) in the presence of UTSAone is 2.4. D. The Km (µM) in the presence of UTSAone is 5.3. The Vmax (pmol/min) in the absence of UTSAone is 6.8 x 103. F. The Vmax (pmol/min) in the presence of UTSAone is 5.0 x 10³. G. The x intercept (µM²) in the absence of UTSAone is -0.186. H. The x-intercept (µM1) in the presence of UTSAone is -0.188. UTSAone binds to a site other than the active site on the dehydrogenase. I.
23. The drug UTSAone was investigated as a possible inhibitor of a dehydrogenase enzyme that acts on pregnenolone as a substrate in steroid synthesis. The activity of the dehydrogenase was measured in the presence and in the absence of 10 µM UTSAone. Use the data in the table to construct a Lineweaver-Burk plot. [Pregnenolone, µM] Vo without I (pmol/min) Vo with I (pmol/min) 1.0 0.00106 0.00079 5.0 0.00327 0.00242 10.0 0.00439 0.00326 20.0 0.00529 0.00395 Which of the following statements are TRUE? A. The Km (uM) in the absence of UTSAone is 2.4. B. The Km (µM) in the absence of UTSAone is 5.4 The Km (µM) in the presence of UTSAone is 2.4. D. The Km (µM) in the presence of UTSAone is 5.3. The Vmax (pmol/min) in the absence of UTSAone is 6.8 x 103. F. The Vmax (pmol/min) in the presence of UTSAone is 5.0 x 10³. G. The x intercept (µM²) in the absence of UTSAone is -0.186. H. The x-intercept (µM1) in the presence of UTSAone is -0.188. UTSAone binds to a site other than the active site on the dehydrogenase. I.
Chapter21: Heparin Infusion Calculations
Section: Chapter Questions
Problem 1.3P
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![23. The drug UTSAone was investigated as a possible inhibitor of a dehydrogenase enzyme that acts on
pregnenolone as a substrate in steroid synthesis. The activity of the dehydrogenase was measured in
the presence and in the absence of 10 µM UTSAone. Use the data in the table to construct a
Lineweaver-Burk plot.
[Pregnenolone, µM]
Vo without I (pmol/min)
Vo with I (pmol/min)
1.0
0.00106
0.00079
5.0
0.00327
0.00242
10.0
0.00439
0.00326
20.0
0.00529
0.00395
Which of the following statements are TRUE?
A. The Km (uM) in the absence of UTSAone is 2.4.
The Km (uM) in the absence of UTSAone is 5.4
В.
The Km (uM) in the presence of UTSAone is 2.4.
D. The Km (µM) in the presence of UTSAone is 5.3.
The Vmax (pmol/min) in the absence of UTSAone is 6.8 x 103.
F. The Vmax (pmol/min) in the presence of UTSAone is 5.0 x 103.
С.
Е.
G. The x intercept (uM1) in the absence of UTSAone is -0.186.
H. The x-intercept (µM) in the presence of UTSAone is -0.188.
UTSAone binds to a site other than the active site on the dehydrogenase.
I.
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Transcribed Image Text:23. The drug UTSAone was investigated as a possible inhibitor of a dehydrogenase enzyme that acts on
pregnenolone as a substrate in steroid synthesis. The activity of the dehydrogenase was measured in
the presence and in the absence of 10 µM UTSAone. Use the data in the table to construct a
Lineweaver-Burk plot.
[Pregnenolone, µM]
Vo without I (pmol/min)
Vo with I (pmol/min)
1.0
0.00106
0.00079
5.0
0.00327
0.00242
10.0
0.00439
0.00326
20.0
0.00529
0.00395
Which of the following statements are TRUE?
A. The Km (uM) in the absence of UTSAone is 2.4.
The Km (uM) in the absence of UTSAone is 5.4
В.
The Km (uM) in the presence of UTSAone is 2.4.
D. The Km (µM) in the presence of UTSAone is 5.3.
The Vmax (pmol/min) in the absence of UTSAone is 6.8 x 103.
F. The Vmax (pmol/min) in the presence of UTSAone is 5.0 x 103.
С.
Е.
G. The x intercept (uM1) in the absence of UTSAone is -0.186.
H. The x-intercept (µM) in the presence of UTSAone is -0.188.
UTSAone binds to a site other than the active site on the dehydrogenase.
I.
w m
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