17 - 6 Why are quantitative analytical methods based on NIR radiation often more precise and accurate than methods based on mid - IR radiation? 17-6 Why are quantitative analytical methods based on NIR radiation often more precise and accurate than methods based on mid-IR radiation?
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- The chromium in an aqueous sample was determined by pipetting 10.0 ml. of the unknown into each of five 50.0-mL volumetric flasks. Various volumes of a standard containing 12.2 ppm Cr were added to the flasks, following which the solutions were diluted to volume. Unknown,mLStandard, mLAbsorbancc 10.00.00.201 10.010.0 0.292 10.020.0 0.378 10.030.0 0.467 10.040.0 0.554 (a) Plot the data using a spreadsheet. (b) Determine an equation for the relationship between absorbance and volume of standard. (c) Calculate the statistics for the least-squares relationship in (b). (d) I)ctcrmine the conccnt ration oÍCr in ppm in the sample. (e) Find the standard deviation of the result in (d).Exactly 5.00 mL aliquots of a solution containing analyte X were transferred into 50.00-mL volumetric flasks and the pH of the solution is adjusted to 9.0. The following volumes of a standard solution containing 2.00 µg/mL of X were then added into each flask and the mixture was diluted to volume: 0.000, 0.500, 1.00, 1.50 and 2.00 mL. The fluorescence of each of these solutions was measured with a fluorometer, and the following values were obtained: 3.26, 4.80, 6.42, 8.02 and 9.56, respectively. ii. Using relevant functions in Excel, derive a least-squares equation for the data, and use the parameters of this equation to find the concentration of the phenobarbital in the unknown solution.In the HQS-Mg fluorescence part of Lab 6, a calibration curve is made of fluorescence intensity vs. MgCl2 concentration (M). The standard solutions for the calibration curve were made by mixing a small amount of 0.01 M MgCl2 with some HQS solution. Calculate the concentration of MgCl2 (M) in each of the following standards. Report your concentrations to 3 sig figs. Fluorescence Volume of 0.01 M Volume of 1 mM Concentration of MgCl2 (M) Intensity MgCl2 (mL) HQS (mL) 0.031250902 0.8 100 0.049490754 0.8 50 0.059803507 2 100 0.085834029 3.6 100 Hint: The total volume of each solution is volume of 0.01 M MgCl2 + volume of 1 mM HQS.
- Quinine in a 1.553-g antimalarial tablet was dissolved in sufficient 0.10 M HCl to give 250 mL of solution.A 10.00-mL aliquot was then diluted to 50.00 mL with the acid. The fluorescence intensity for the dilutedsample at 347.5 nm provided a reading of 196 on an arbitrary scale. A standard 100-ppm quinine solutionregistered 125 when measured under conditions identical to those for the diluted sample. Calculate the massin milligrams of quinine in the tablet.To quantify the Cadmium content in a water sample, 5 aliquots of 10 mL were taken, adding to each one of them a volume of a standard solution of Cd (2 mg / mL); after the addition the final volume for all solutions was 50 mL. The absorbance reading was taken in an Atomic Absorption spectrophotometer, the data recorded were: Aliquot 1 2 3 4 5 Cd solution (mL) 0 1 2 3 4 Absorbance 0.163 0.240 0.319 0.402 0.478 Calculate the ppm of Cd.What is difference between SPE and DSPE?
- The quenching of tryptophan fluorescence by dissolved O₂ gas was monitored by measuring the emission lifetimes at 348 nm in aqueous solutions. [0₂]/(10-² mol dm-³) T/(10-⁹ s) 0 2.3 5.5 8 10.8 2.6 1.5 0.92 0.71 0.57 A. 0.95 E-2 M B. 4.3 E-2 M C. 1.8 E-2 M D. 3.0 E-2 M E. None of the choices From the data above, predict the value of [0₂] required to decrease the intensity of tryptophan emission to 50% of the unquenched value.Gb6cd_i-5c7IV_IVquz8U4UO2epq7z4kBnpYZ5iA/edit > 2 ons Help Calibri - - [12] + B 7 U . UA 2 3 ge 2: Problems of Water and Air Quality Questions 1. Why is it recommended to have a carbon monoxide detector on each floor of a home? Et © ª @ E- E 4-B-E- 2. What impact would the increased use of electric vehicles have on environmental damage caused by acid rain? 3. Why is ground level ozone most likely to reach its highest concentrations on hot, sunny days during periods of heavy traffic in large cities? Page 3: More Air Pollution Issues: Questions 4. The COVID-19 pandemic forced many people to work from home for extended periods. During these periods, many cities reported unusually low levels of smog. Suggest an explanation for this observation. Page 4: Water Pollution: Questions 5. Explain how ozone can be both harmful and beneficial. 6. India relies heavily on burning coal to generate electricity. How does India's reliance on coal contribute to the poor air quality experienced in many of…4 of 15 Abs 2.0 1.5 1.0 0.5 0.25 0. 0.2 0.4 0.6 Concentration (M) With reference to the UV-Vis calibration curve reported above, calculate the concentration (Molar) of a sample whose Absorbance is 1.0. Report your answer with two significant figures.
- In the process of performing a spectrophotometric determination of Fe, an analyst prepares a calibration curve using a single-beam spectrometer, such as a Spec-20. After preparing the calibration curve, the analyst drops the cuvette used for the method blank and the standards. The analyst acquires a new cuvette, measures the absorbance of the sample, and determines the %w/w Fe in the sample. Will the change in cuvette lead to a determinate error in the analysis? Explain.one: Topic Molecular Spectroscopy (16 marks) You were given an unknown mixture that contains two metal salts (A) Co(NO3)2 and (B) Cr(NO3)2 and requested to determine their concentration. The following representative information about the absorbance data were also provided. Measurements were made in a 1.0 cm quartz cells. Pure A (mol/l) Pure B (mol/l) 0 Abs @510 nm Abs @575 nm 1.5 x 101 0 6 x 102 Unknown conc in mixture 0.714 0.097 0.298 0.757 Unknown conc in mixture 0.671 0.330 How would you go about calculating the concentration of the two salts?You obtained the following raw data when setting up a Biuret standard curve: Absorbancy 540nm BSA (mg/ml) 0.158 1 0.210 2 0.260 3 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.279 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).