1) Provide a stepwise, arrow-pushing mechanism for the following transformation. You may use general acid and base as necessary. Feel free to abbreviate structures as needed. OH SCOA Lyase TPP O H H O SCOA
Q: what metabolic pathways are more likely to be active during: a) fasting, b) overnight fast) and c)…
A: Intermediary metabolism, also known as metabolic pathways, is the intricate network of chemical…
Q: 4. a. For an exponentially growing population, calculate umax (units?) based on the data below Time…
A: Monod kinetics describe the relationship between the growth rate of microorganisms and the…
Q: 2. Shown below is the enol intermediate in the citrate synthase mechanism. Draw the carbanion…
A: Citrate synthase is an enzyme in the citric acid cycle. It catalyses the condensation of acetyl CoA…
Q: Dihybrid Cross 13 Problem 5: A SsYy x ssyy test cross. Which of the following genotypes would you…
A: The objective of this question is to determine which genotype would not be expected in the offspring…
Q: a. How many peptide bonds are there? b. How many amino acid residues are there? c. In exact order,…
A: As per Bartelby Guidelines, we can answer only three subparts of a question. So, kindly post other…
Q: The carboxylation of acetyl CoA to synthesize malonyl CoA by acetyl CoA carboxylase requires…
A: One of the B vitamins is biotin, sometimes referred to as vitamin H or B7. Both in humans and other…
Q: Free energy diagrams are super fun to draw! Draw a set of free energy diagrams that show the…
A: Free energy diagrams have free energy (G) in Y-axis and reaction coordinate in X-axis. These can…
Q: Create a figure that includes a schematic of the topological map of AlaE. In your topological map,…
A: Topology data from 3 different techniques; hydropathy profile analysis, TMHMM and TOPCONS are…
Q: In two sentences describe alternate splicing and why it is important
A: Alternate splicing is a process that occurs during gene expression, where the exons of a pre-mRNA…
Q: Which of these situations would produce a Hill plot with a Hill coefficient less than 1? A purified…
A: A Hill plot is used to establish the cooperativity of ligand binding to a protein.NH (Hill…
Q: Draw a parallel beta sheet and an antiparallel beta sheet (three strands each). Show the hydrogen…
A: The secondary structure of protein refers to the local folded structure that forms within in…
Q: Draw the chemical structure for a phosphatidylethanolamine which contains trans, trans, cis-Δ10, Δ15…
A: I can describe the structure for you. The phosphatidylethanolamine molecule with trans, trans,…
Q: Question 9 Listen Two hamsters with black, short hair mate. They have the following offspring 18…
A: The objective of the question is to determine the genotypes of the parent hamsters based on the…
Q: Sketch a typical enzyme kinetic curve of [S] vs. V. The maximum [S] should be about four times the…
A: In a typical enzyme kinetic curve, the substrate concentration, [S] is plotted along the x-axis and…
Q: Draw the amino acid below in its most protonated form, any intermediately protonated forms, and its…
A: pH = 1/Log [H+] If [H+] concentration in a solution is high, pH will be low and the solution is…
Q: Referring to the figure below, which letter (s) indicate (s) the site (s O a. A+B O b. Only B O c.…
A: Methylxanthines: They are a group of naturally occurring compounds like caffeine, theophylline, and…
Q: In experiments carried out in 1941 to investigate the citric acid cycle, oxaloacetate labeled with…
A: The given reactions are part of a metabolic cycle known as the citric acid cycle or Krebs cycle. The…
Q: D raw the full structure of the following tetrapeptide . Label the N terminal and C terminal…
A: An amino acid is a biomolecule that has a hydrogen atom, an amino group, a carboxyl group and a…
Q: Dihybrid Cross Problem 9: Homozygous offspring of a dihybrid cross, again. In a dihybrid cross,…
A: The objective of this question is to determine the fraction of offspring that will be homozygous for…
Q: You are studying how your Lys-Val-Thr tripep de interacts with another pep de, which places an Asp…
A: Eventhough all ionizable groups have their characteristic pKa value, the pKa value of an ionizable…
Q: A protein has a molecular mass of 400 kDa when measured by size-exclusion chromatography. When…
A: Size exclusion chromatography is a technique which separates molecules according to their size…
Q: Question #4 The graph on the left shows data for an experiment in which isolated mitochondria were…
A: The process of oxidative phosphorylation, which mitochondria use to produce energy, is essential to…
Q: Consider the image of the amino acid. Identify the amino acid. Isoleucine Glycine Alanine Leucine…
A: An amino acid is an organic molecule that is made up of a basic amino group (−NH2), an acidic…
Q: After running a qPCR experiment, we will have graphs showing the amount of fluorescence detected by…
A: The Polymerase Chain Reaction is a qualitative procedure which is used to produce large copies of…
Q: a. Write the structure of a-D-fructose.
A: Since you have posted multiple questions, we will provide the solution only to the first question as…
Q: Which of the sugars below are diastereomers of compound I? CHO H+ OH H-+OH H+OH CH₂OH I CHO H-OH…
A: Stereoisomers are the type of isomers in which the chemical formula of the molecules as well as the…
Q: 2. Selective synthesis of the tetrapeptide Ala-Val-Gly-Leu a. Work backward from Structure K to fill…
A: Peptides can be synthesized chemically. For this we use various reagents like Boc (also called…
Q: If non-disjunction occurs at meiosis 2 in a male, 2 of the 4 sperm formed in that meiosis will have…
A: Non-disjunction can happen in meiosis 1 or meiosis 2.Non-disjunction is said to have taken place in…
Q: Draw the structure of phosphatidylcholine at pH 7 with the moiety on R₁ as 14:0 (C14) and R₂ as…
A: Glycerophospholipids, membrane lipids in which two fatty acids are attached in ester linkage to the…
Q: Monosaccharides have the general molecular formula of (CH₂0)m, where m is the number carbons. (m=5…
A: Starch is formed as a result of polymerization of glucose. This is also a condensation reaction as a…
Q: Name the protein structural element shown in yellow in the figure:
A: Proteins can be defined as large biomolecules. They comprise of one or more chains of amino acids.…
Q: Produces Leaves the nucleus and goes to the... 7. Transcription and Translation---DNA: A A…
A: Piece's of RNA make amino acids, which make proteins and this process occurs in the Cytoplasm. m RNA…
Q: What is the difference in ATP yield per glucose molecule between the malate-aspartate shuttle and…
A: During glycolysis of glucose, 1 molecule of glucose yields 2 molecules of NADH. The high energy…
Q: How many TCA cycles required to fully oxidize 1 molecule Acetyl-CoA. Support your answer with…
A: There are two oxidizable carbons in acetyl-CoA; the methyl carbon (CH3-) and the carbonyl carbon…
Q: Genetics Question 3
A: The objective of the question is to understand whether epistasis and dominance are the same concepts…
Q: Question 7 Identify the functional groups in the following structures. HO 1. 12345 1. HO- 3. GAWNE…
A: There are various functional groups present in each organic molecule. A functional group is a group…
Q: In the alpha helix shown below, all carbonyl groups are pointing_ amino groups are pointing…
A: The secondary structure of a protein refers to the folded configuration of the polypeptide chain.…
Q: Ala-Cys-Glu - Tyr - Trp - Lys - Arg - His - Pro-Gly NAZO Glu pka 4.15 SH Tyr RO 10.10 Draw Charges…
A: Proteins are essential biomolecules composed of amino acids arranged in four structural levels:…
Q: 4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You…
A: Enzymes are the biocatalysts that catalyse the biochemical reactions by lowering the activation…
Q: What did experiments using porcelain filters prove? Select all answers that apply. Porcelain filters…
A: Porcelain filters were engineered in the late 19th century by scientists as bacteriological filters…
Q: Which of the following are features of secreted bacterial proteins? (SELECT ALL THAT APPLY) A…
A: The question is asking about the features of secreted bacterial proteins. Secreted bacterial…
Q: 1. ATP ACCOUNTING, Provide what is being asked for. Show all relevant calculations and summarize…
A: Fatty acids are organic compounds determined by a long hydrocarbon chain having a carboxylic group…
Q: Genetics Question 1
A: The question is asking whether genes located on different chromosomes are inherited independently of…
Q: You have isolated a new trisaccharide from a new species of insect shown below that you are calling…
A: The dashed bonds will come below the plane of the ring and the wedge bonds will become above the…
Q: 5. What is a saponification number? What does a high saponification number indicate? Name a lipid…
A: Biological macromolecules are the molecules that are required in sufficient quantity for the…
Q: What is the target for the following compound? O Muscarinic receptor O Acetylcholinesterase enzyme O…
A: Acetylcholine is a neurotransmitter. Many enzymes and proteins involved in the metabolism and…
Q: Answer the following questions with regards to DNA replication: 1. In what direction does DNA…
A: DNA replication is the process of duplication of DNA by using the parent strands as template strands…
Q: The following question focuses on how the parameters regulating enzyme function might change, and…
A: Michaelis-Menten (MM) plot and Lineweaver Burk (LB) plot are drawn to decipher the kinetic…
Q: Please fill in the four boxes bolded below you KM (MM) Vmax (nM/s) Type of inhibition…
A: Competitive inhibitors are the substances which are similar to substrate molecules in their…
Q: (a) AG for transport of a proton from the mitochondrial matrix (inside) to the intermembrane space…
A: Where,C( inside)= Concentration of H+ ions inside the mitochondrial matrixC(outside)= Concentration…
Step by step
Solved in 3 steps with 2 images
- 1) Provide a stepwise, arrow-pushing mechanism for the following transformation. You may use general acid and base as necessary. Feel free to abbreviate structures as needed. مسلسلند SCOA Lyase TPP mos مسلسلند H + H 요 SCOASketch the appearance after visualization of a protein mixture containing the seven proteins (fibrinogen, y-globulin, collagen, ovalbumin, myoglobin, hemoglobin, insulin) when subjected to two-dimensional (2D) gel electrophoresis.a. An oligopeptide ALVGALGATPTPQMWSHSWRGVSIKS was digested with trypsin.Which method would be most appropriate for separating the products: ion exchange or gel filtration chromatography? Explain.b. Suppose that the peptide was digested with cyanogen bromide. What would be the optimal separation technique? Explain
- Explain why protein purification procedures are normally carried out 4-5°C.protein is purified and at a concentration of 600 μg in 1.75 ml of buffer. You do an assay with your purified protein, and the assay requires 25 μg of biochemisfunase. What volume of the purified biochemisfunase would yield 25 μg? Show your calculation.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- Please provide a separation solution of combining two types of chromatography to isolate the protein A from the mixture containing protein A, B, C and D (shown as below). And then explain the related mechanisms in details. Protein A: Met-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu- Protein B: Met-Asp-Glu-Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu Protein C: Met-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu-Val-Val-Val- Ile-lle-lle-Leu-Leu- Leu-Leu-Val-Val-Val-lle-lle-lle-Leu-Leu-Leu-Leu-Val-Val-Val-lle-lle- lle-Leu-Leu-Leu-Leu-Val-Val-Val-Ile- Protein D: Met-Asp-Glu--Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu-Glu-Asp- Glu-Asp-Glu-Glu-Asp-Asp-Glu-Glu-Glu-Glu-Asp-Asp-Asp-Glu-Glu-Asp-Asp-Glu-Glu- Asp-Glu-Asp-Glu-Glu-Asp-Glu-Glu-Asp) In urea assay (Catalog #K375-100) what is the nmol (range) it can detect?39. The most expensive method to purify the protein is: Affinity Size exclusion RP-HPLC (reversed phase) lone exchange
- (a) The octapeptide AVGWRVKS was digested with the enzyme trypsin . Which method would be most appropriate for separating the products: ion-exchange or gelfiltration chromatography? Explain. (b) Suppose that the peptide was digested with chymotrypsin . What would be the optimal separation technique? Explain.What is the practical advantage of the approximate red cell suspension over the exact red cell suspension?Design an assay with purified phycocyanin to test protein stability using 500ml of 95% ethanol. Then do the same with 2 M sodium chloride. Make each step clear.