4. a. For an exponentially growing population, calculate umax (units?) based on the data belowTime (d)Biomass (mg/L)01275111166248369b. Calculate the specific growth rate (µ) of the population above growing at a substrateconcentration of 25 mg/L according to Monod kinetics with a ks of 50 mg/L.c. Consider two genetically engineered organisms intended for use in the cleanup of hydrocarbonspills into the ocean. Bacterium alpha has a µmax of 1 d'¹ and a ks of 0.1 mg/L. Bacterium beta hasa μmax of 5 d ¹ and a k, of 5 mg/L. If the hydrocarbon concentration is initially 1000 mg/L and thedesired objective is to reduce concentrations below 0.1 mg/L what order of addition of the bacterialspecies is desired?35045

Monod kinetics describe the relationship between the growth rate of microorganisms and the…

Answered: 4. a. For an exponentially growing…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Suggest TWO (2) commonly available biomass in Malaysia that can be utilized as potential feedstocks for fermentation and discuss the importance of feedstock pre-treatment. ⠀⠀
Using the data below from a batch test, estimate the observed yield and the decay rate of an organism growing on toluene as a substrate. Show / explain your work. Be sure to include units. Time (hrs) Toluene (mg/L) Biomass (mg VSS/L) 300 100 2 200 150 4 100 200 8 25 250 16 5 252 24 0.1 248 36 0.1 230 48 0.1 220
The case study for UPSTREAM AND DOWNSTREAM PROCESSING TECHNOLOGY needs to be conducted to study about Two different types of microorganisms producing similar bio-based compound using similar substrates. Microorganisms selected: Saccharomyces cerevisiae vs Zymomonas mobilis Bio-based compund: Ethanol QUESTION: Please explain very clearly about the Medium formulation (1.what are the substrates?,2.pre-treatment of substrates, 3.addition of stimulant or etc., 4.advantages and disadvantages of the selected substrates).
A batch reactor was initially filled with organic substrate (S0) with an equivalentconcentration of COD = 1000 mg/L and 100 mg/L of bacterial biomass (X0). After 24 hraeration, the biomass in the reactor increased to 300 mg/L, while the substrateconcentration decreased to 200 mg/L as of COD; what is the observed biomass yield (Y)during this biodegradation process?
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Two microorganisms that use naphthalene as a food source have the same Umax but different Ks values for naphthalene. If both types of microorganisms are inoculated at the same cell density into a liquid culture containing naphthalene as the sole carbon source (i.e. the only food source), which microorganism will outcompete the other: the one with the higher Ks or the one with the lower Ks. Why?.
Rate in U [S] in mM No Inhibitor 0.5 1.0 2.0 4.0 8.0 16.0 32.0 64.0 0.22 0.41 0.73 1.12 1.57 2.01 2.3 2.5 3.0 mM Inhibitor Copy and paste the above data into Excel. Create an Eadie-Hofstee plot of the given dataset. 0.08 0.15 0.29 0.52 0.85 1.3 1.78 2.16 Calculate the Vmax, and Vmax/ Km parameters for both the uninhibited and inhibited trials (Inhibited parameters are marked with an apostrophe (')). Finally, determine the mechanism of inhibition. Note: give all parameters to two decimal places, and do not include the units. For the method of inhibition, state competitive, uncompetitive, or mixed as your answer. U. Vmax/Km- A U. Vmax/Km= Vmax 1. Vmax= U-mM-1. Mechanism of inhibition = inhibition A/ A/ A/ U.mM A
The case study for UPSTREAM AND DOWNSTREAM PROCESSING TECHNOLOGY needs to be conducted to study about Two different types of microorganisms producing similar bio-based compound using similar substrates. Microorganisms selected: Saccharomyces cerevisiae vs Zymomonas mobilis Bio-based compund: Ethanol QUESTION: Please explain and discussion very clearly about the advantages and disadvantages of both of the microorganisms in paragraph form.
For scenario 2, below write the following 1.the process that the sets of measurements represent. 2.suitable equations of the process. 3.the calculated rates and 4.the resulting units for the process as expressed by your calculation. Scenario 2 Time,0 minutes:7.13mg/L O2 Time,20 minutes:13.39mg/L O2 Algal biomass:2.3g fresh mass

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