You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
Q: As shown in the image below, 10 µl of a stock solution (tube #1) are serially diluted into 0.990 ml…
A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…
Q: A saline solution (used in intravenous drips for patients who cannot take oral fluids) contains…
A: The normal saline is 0.9%. This means that there is 0.9 g of salt (NaCl) per 100 ml of solution, or…
Q: Of a 10%6 SDS solution in H20. The combined final solution would be 0.5M NaC1 and 1% SDS. If you are…
A: Molality is never equal to molarity. But the difference becomes lesser as the solutions become more…
Q: A glucosamine solution of unknown concentration was diluted as follows: 1 mL of the solution was…
A: Glucosamine is a polysaccharide found in the outermost structure of chitinous animals. In humans, it…
Q: What is the relationship of concentration and absorbance based on the table and what are the changes…
A: The concentration of a solute in a solution can be determined spectrophotometrically by passing a…
Q: For the following, calculate pka, and pka, of 1 Glycine, determine the 2 buffering regions, and…
A: Glycine is an amino acid with H-atom as the side chain. The isoelectric point of a amino acid (pI)…
Q: 40.00 mL of 0.1100 M HCl is diluted to 100 mL with H2O and titrated with 0.1M NaOH. Calculate the pH…
A: pH is generally a method to find acid or base. The ranges from 0 to 14, with 7 being neutral. pHs of…
Q: Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of…
A: Beer Lambert's plot It's a graph showing a compound's absorbance vs. concentration. It is a straight…
Q: Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the…
A: Lambert Beer law states that Absorbance of a solution is directly proportional to its concentration…
Q: volumetric flask made of Pyrex glass is calibrated at 20 °C.It is filled to the 100 mL mark with 35…
A: Original volume is 100 mL. Delta T = 20 - 35 = -15°C After cooling to 20 °C, the volume is Vfinal=…
Q: When 0.2 millimoles of Y are dissolved in 250 ml of water, the resultant solution has an absorbance…
A: Beer-lambert law states that for the given material, the sample concentration and path length are…
Q: As a technician in a large pharmaceutical research firm, you need to produce 200. mL of 1.00 M…
A: Given Values: pH of the potassium phosphate buffer required = 6.96 pKa value of the potassium…
Q: You need to prepare an acetate buffer of pH 5.43 from a 0.621 M acetic acid solution and a 2.95 M…
A: At equilibrium 0.40875 - x moles of acid x moles of its conjugate base. Initial moles of CH3COOH…
Q: The unknown metal sulfates are hygroscopic and will absorb water from air. The unknowns must thus be…
A: Hygroscopic properties of sulfate-containing metals are crucial to understanding the atmospheric…
Q: How much water must be added to 300 mL of an aqueous solution of 0.2 M acetic acid in order to…
A: Given : 0.2 M acetic acid solution , volume 300 ml Let C1 = 0.2 M C2 = concentration of acetic acid…
Q: The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L =…
A: Asked : Effect on measured pH value if acid concentration is increased HCl is an acid
Q: If the equation produces from data is y = 0.300x - 0.857 and the absorbance for the unknown is…
A: Note: Since you have posted multiple independent questions in the same request, we will solve the…
Q: Which of the following reagents will you include in the blank (negative control) for this…
A: A variable is any quantity we can measure. In any experiment there are three variables:…
Q: For the TylenolTM dose, calculate how much (in mg) did Erthy consume for all six doses. To do that,…
A: Drug computations practice will help caregivers build better and more assured arithmetic abilities.…
Q: If you had measured the absorbances of your unknown in test tubes that were half the diameter of the…
A: Proteins are composed of twenty standard amino acids that are attached together via peptide bonds.…
Q: If your initial absorbance from undiluted crude dialysate is 4.50, determine the fold dilution…
A: Since you have posted multiple questions, we will answer the first one for you. If you want a…
Q: You will be given a stock solution of the dye EVANS BLUE dissolved in distilled water whose…
A: Scientists use molarity to describe the concentrations and it tells us how much solute is dissolved…
Q: You need to prepare 1L of a 5×TE stock solution (1×TE buffer:10mM Tris,1mM EDTA).How much of Tris…
A: The mole is used as a unit in the SI system to quantify the amount of substance present. One mole is…
Q: You have a 6 M solution of stock A. You do the following serial dilutions: Add 1 ml of stock A to…
A: Dilution is calculated by stock volume to the final volume. Solution = Stock volume/ (stock volume+…
Q: 12- 10- 8- 6- 4- 2- 0- 20 40 60 80 Volume of NaOH (mL) Hd
A: The titration curve is the graphical representation of pH. The pH is greater than 7 for weak acid…
Q: Calculate the enthalpy of vaporization at the normal boiling point (760 mmHg) of the liquid(water,…
A: The enthalpy of vaporization also known as heat of vaporization or heat of evaporation is the amount…
Q: Calculate the protein concentration (in mg/ml) of an unknown sample with an absorbancy value of…
A: Bradford method of protein estimation is quick and simple method of protein estimation using…
Q: What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock…
A: Molarity is defined as the number of moles dissolves in 1000 ml of the solution.
Q: The pH value is calculated mathematically as the Log of the .hydrogen ion (H+) concentration False…
A: The pH of a solution is used as an indicator of the hydrogen ion concentration of the solution. A…
Q: A KCC student titrated 100.00 mL of a NaOH solution using 55.00 mL of a 0.1000M H2SO4 solution. How…
A: The reaction of an acid with a base is called an acid-base reaction. It is also called a…
Q: Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of…
A: Spectrophotometry enables us to calculate the concentration of a species in a solution by measuring…
Q: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 2 0.420 3 0.510 4 0.610 0.720 6 0.810 7 0.915 8 0.950…
A: The Bradford Assay is is a sensitive assay to detect and quantify protein in solution.
Q: An aqueous solution is 3.50 % by mass hydrochloric acid, HCl, and has a density of 1.02 g/mL. The…
A: Mole fraction is expressed in the terms of moles. It is can be calculated from the formula that is…
Q: Make a standard curve by using the values given in the table and determine the concentration of…
A: Bovine serum albumin or BSA is used for standard curve preparation because of its stability. The…
Q: 1. compute for patient B result of his chloride analysis with the following data using mg/dL unit…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions…
A: Standard curves are graphs of absorbance v/s concentration, It is used to find out the solute…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5…
A: Even when the concentration of BSA (Bovine Serum Albumin) is 0 , an absorbance of 0.158 was…
Q: During transfer of liquids using a micropipette:
A: Micropipettes are used in laboratory to measure volumes less than 1000 uL or 1 mL.
Q: The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L =…
A: Asked: Correct option out of the given options
Q: What volume of 0.10NH2 SO3 will be required to neutralize a solution containing 10.0grams of…
A: Given Values: Weight of Ca(OH)2 = 10 grams The molecular weight of Ca(OH)2= 74 Normality of H2SO4 =…
Q: A 1.0-L 0.010 M buffer with pH 6.50 is given as an assignment to a group of students. Which is the…
A: A weak acid is used to prevent any change in the pH of the solution, regardless of the solute. On…
Q: Calculate the mass of a liquid with a density of 3.2 g/mL and a volume of 25 mL
A: The amount of matter present in a substance is defined as its mass. It is independent of its…
Q: Calculate the followings: The pH of the final solution after adding 300 ml of 40 mM NaOH to 600 ml…
A: pH is the scale that is used to specify the acidity or basicity of an aqueous solution. Acidic…
Q: By a stoichiometric calculation, determine the pH of a buffer solution prepared by dissolving 25.5 g…
A: The equation that relates the pH of an aqueous solution of an acid to the acid dissociation constant…
Q: Calculate the molarity of dilute Ca(OH)2 solution if the titration of 30.00 mL of 0.05231 M HCl…
A: Given : 30.00 mL of 0.05231 M HCl For this , n 2=1 as 1 hydrogen ion is present V2= 30 ml M2 =…
Q: 10 Determine the Hill coefficient for the following binding data using a linear method. [L] in…
A: The Hill plot is a graph graph which enables us to check whether the protein in question exhibits…
Q: If a sukfuric acid solution is titrated with the above sodium hydroxide soultion, it reqires 45.20…
A: Acid-base titration: This is a standard procedure to measure the concentration of unknown acid/base…
Q: You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy…
A: The Biuret test is used to find the amount of protein in the given analyte as it checks the presence…
Q: A 1–fluid ounce (29.573 mL) solution contains 4.5 grains (291.60 mg) of silver nitrate. How much…
A: Isotonicity of nasal fluid is similar to 7 % of sodium chloride, NaCl.7% of NaCl means 7 grams of…
You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- i) Write down the equation derived from your Excel generated standard curve , figure legend and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precisionA 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).Consider the titration of 50.00 mL of 0.160 M NH3 with 0.200 M HCl. Calculate the pH at the following volumes of the titrant: VT = 0.00 (Initial Stage) VT = 20.00 VT = 30.00 VT = 40.00 (Equivalence Point Stage) VT = 45.00 Kb = 1.76 x 10-5
- What would be the working stock concentration (in molarity) if you diluted 250 uL of a stock concentration of 10 uM in a total volume of 1 mL?BSA Concentration (ug/mL) Abs 2000 2.25 1500 1.715 1000 0.915 750 1.156 500 0.714 250 0.082 125 0.086 25 0.082 Create a BSA standard curve Abs562=f[BSA] using a Scatter Plot representation in Excel.You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 10 0.550 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).
- You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.262 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).A mixture containing only KCl and NaBr is analyzed by the Mohr Method. A 0.3172-g sample is dissolved in 50 mL water and titrated to the Ag2CrO4 endpoint, requiring 36.85 mL of 0.1120 M AgNO3. A blank titration requires 0.71 mL of titrant to reach the same endpoint. Report the % (w/w) KCl and NaBr in the sample. [Ans . 84.41 % (w /w); 17.59 % (w /w)]A concentrated lidocaine solution contains lidocaine 1 g/5 mL. How many milliliters of this injection should be used to prepare a 500 mL IV infusion containing 8 mg/mL of lidocaine in D5W? Do not round.
- You dissolve a 3.435 gram sample of potato in acid to determine the levels of solanine (the reason why green potatoes are poisonous) and diluted it to a total volume of 500.0 mL. You have also created a standard curve as outlined below. Solve for the ug/gram concentration of solanine in the potato sample. ppm Solanine Std. Abs 0.213 5 0.315 10 0.423 15 0.532 Unknown sample 0.314 Your Answer:A buffer contains 0.015 mol of lactic acid (pK₁ = 3.86) and 0.080 mol of sodium lactate per liter. H₂C OH Lactic acid OH Calculate the pH of the buffer. H₂C. Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of the buffer. O OH Sodium lactate Calculate the change in pH after adding 9.0 mL of 0.10 M HCl to 1.0 L of pure water. O Na+ buffer pH: buffer pH change: water pH change: units unitsThe pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if HCl in pH electrode, became 0.15M instead of 0.1M ? a.impact can not be determined b.measured pH is higher than "real" pH. c.measured pH is lower than "real" pH. d.measured pH is same as "real" pH.