You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis.  You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc.    You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies.  How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flask

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter2: Multiplication And Division Of Decimals
Section: Chapter Questions
Problem 6.8P
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You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis.  You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc.    You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. 

How many bacteria (measured in CFU/mL) were in the overnight culture flask?

A. 1.34 x 10^4 CFU/mL

B. 1.34 x 10^5 CFU/mL

C. 1.34 x 10^6 CFU/mL

D. 1.34 x 10^7 CFU/mL

E. 1.34 x 10^8 CFU/mL

F. cannot tell based on the data given - you'd need to know the volume of the original culture flask

Performing Serial Dilution
Imt
+9ml
1/10
(/1000)
(1/10000)
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Transcribed Image Text:Performing Serial Dilution Imt +9ml 1/10 (/1000) (1/10000) www.technologyinscience.blogspot.com
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