You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium botulinum in commercial sterilization. Calculate F value when sterilized at 131 °C in canning processing.
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You are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium botulinum in commercial sterilization. Calculate F value when sterilized at 131 °C in canning processing.
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- Identify the method of sterilization/ chemical agent described At 15psi, the temperature reaches 121 degree Celsius requiring only 15 to 20 minutes to sterilize the material. The material to be sterilized is exposed to live steam for 30 minutes for 3 consecutive days.Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiDisinfectants and Sterilization Procedures Complete the table by putting (+) for growth of microorganisms or (–) absence of growth of microorganisms. Disinfectant Concentration Growth after time of exposure (minutes) Control 5 minutes 10 minutes 15 minutes Sodium hypochlorite 5% Sodium hypochlorite 0.05% Alcohol 70% Alcohol 40% Iodophor (Betadine) 10% solution Mouthwash Cetylpyridinium Chloride - 0.075% Sodium Fluoride - 0.05%
- Alternative name for Moist-heat sterilization Select one: Oven sterilization Autoclaving Ionizing radiationSweet sorghum juice is used as sole carbon source for ethanol production by Saccharomyces cerevisiae. The overall in situ sterilisation duration is 40 minutes at 120 0C for a pilot reactor (50 L) which is filled with 60% of working volume. The non-sterile medium consists of 3.28 x 106 contaminants L-1. During the autoclaving process, you have noticed that the time taken for heating and cooling are 18 minutes and 15 minutes, respectively. As a process engineer, explain if you agree with this sterilisation practice by using quantitative analysis.List 5 other methods of sterilization and describe the principle/s and when each method is used. What are the requirements to support microbial growth?
- Other than the application of heat, name other methods of sterilization. Define the methods and give examples of materials sterilized in each method.Oven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect: Rationale:For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise when the slide is heated in a flame?
- (b) A Food material containing Bacillus stearothermophilus PS1518 as an indicator organism ts subjected to heat sterilization at 121 C. Calculate the time required to reduce the organism to one tenth of the original number. Fo value for the organism is 4 minutes and the decimal reduction time , D, at 116°C is 40 minutes. Assume operation is at constant temperature of 121°C. HINT Fo = -To 10 dt Where Fo = equivalent exposure time at 121°C of the actual exposure time at a variable temperature To = the reference temperature =121 C, z=10 = number ofC necessary for10fold increase in FMatch the most appropriate method of sterilisation for the following items. 10 mL vitamin solution 5 mL antibiotic solution Petri dishes 1 L of water 1 L of nutrient broth medium Talcum powder Charcoal powder [Choose ] [Choose ] Gamma radiation Autoclave Dry heat oven Filtration [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]What is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit response