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- Definition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified OrganismDiscuss the uses of PCR and RT-PCR in human identity (e.g. paternity, forensics or criminal cases) as well as in viral or bacterial DNA/RNA analysis (i.e. Identification of SARS-COV-2 RNA coronavirus using RT-PCR).Answer the following questions:1. What are the different sections in the Clinical laboratory? Which one you think, will be your favorite?2. Enumerate the 3 Coronavirus strains that caused a large-scale outbreak in humans in the past 2decades.3. What is the first step that should be done in PCR testing?
- Definition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified OrganismWhich of the following is not required for PCR? a. dNTPs b. bacterial plasmids c. carefully designed primers d. taq or other heat- resistant polymerases e. template DNA24. Arrange the correct sequence in southern blotting. I. DNA extracted from white cells and are cleaved. II. hybridization with p-labeled probe. III. Agarose gel electrophoresis. IV. Blotting technique V. Expose of x-ray film. O A. I, III, II, V, IV ) B.I, II, III, IV, V. OC.I, IV, III, V, II D. I, III, IV, II, V. Clear selection O O O
- Please answer these two questions regarding PCR a) First question you must use the image: What is the DNA profile for suspect B for the TH01 locus? b) What determines the target sequence amplified in PCR?Brenda is a junior student in the biomedical program at her school. She is starting the PCR genetic testing lab activity. She is about to obtain her DNA sample but doesn’t want like the taste of NaCl solution. Her friend, Mark, let her use some of his DNA. What laboratory tule did the students break? A. Obtaining and handling DNA sample without wearing googles or gloves B. Improper use of human DNA samples C. Violating Patient Confidentiality D. Disposing of bio hazardous material in a regular trash3 different pcr tubes were obtained each tube containing a variation of hemoglobin E. tube A is a normal hemoglobin E,tube B is a diseased hemoglobin E, and tube C is a hemoglobin E trait. conduct a graph showing which one would move further electrophoresis gel well if an experiment was performed. explain your answer.
- What are the advantages of real-time PCR over microscopy for diagnosing malaria? a. Giemsa stain is not required for real-time PCR b. It allows for species identification and quantification of malaria-causing plasmodium at lower blood concentration with greater sensitivity and specificity. c. Real-time PCR doesn't require the use of a microscope which is deemed too expensive. d. Real-time PCR has a hiher sensitivity for the main malaria-causing plasmodium, P. falciparum, than microscopy techniques. Thank you!!!1. a) A technologist has a 20µl sample of DNA and adds 5µl of loading dye before adding the total volume into an agarose gel. What was the original concentration of the loading dye? a. 4x b. 5x c. 6x d. 7x e. None of the above 1. b)A technologist adds 6uL of 5x loading dye to a DNA sample before adding the total volume into the agarose gel. How much DNA sample was combined with the loading dye? 12uL 16uL 20uL 24uL 28uLCHOICES: a. Plasmid b. Sticky end c. DNA ligase d. Transformation e. Restriction enzyme f. Genetic marker g. Transduction QUESTIONS: Cuts the DNA into fragments Circular DNA molecule of bacteria Used to insert DNA of interest to vector Area of DNA where bases are ready to be paired Recombinant DNA technology with the help of a vector gene