what would you expect to find in a metagenomic library ? (check all that apply) A. peer reviewed research articles on disease and the environment B. the complete genome from a new species C. bits of DNA from numerous species D. many copies of cloning vector with different inserts E. a composite genome from several related species F. some DNA representing genes that are similar but differ in exact sequence

what would you expect to find in a metagenomic library ? (check all that apply) A. peer reviewed research articles on disease and the environment B. the complete genome from a new species C. bits of DNA from numerous species D. many copies of cloning vector with different inserts E. a composite genome from several related species F. some DNA representing genes that are similar but differ in exact sequence

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 7RQ: What is the most challenging issue facing genome sequencing? a. the inability to develop fast and...

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What is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the above
Which of the following processes can be used to clone animals? Select one: A. fusion of an adult cell's nucleus with an enucleated egg, followed by incubation in a surrogate B. separation of an early stage blastula into separate cells, followed by incubation in a surrogate C. use of mitochondrial DNA from adult female cells of another individual • D. replication and dedifferentiation of adult stem cells from bone marrow
The Human Genome Project organized by many universities and research institutes in 1990 was mainly Select one: a.isolating genes from human DNA b. making many copies of human genome c. making many copies of genes extracted from in human DNA d. sequencing all the bases in human genome DNA e. collecting all proteins from cells in a population
Which of the following processes can be used to clone animals? Select one: A. fusion of an adult cell's nucleus with an enucleated egg, followed by incubation in a surrogate B. separation of an early stage blastula into separate cells, followed by incubation in a surrogate C. use of mitochondrial DNA from adult female cells of another individual D. replication and dedifferentiation of adult stem cells from bone marrow
A has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choice
With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.
For each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.
Arrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,b
What is a transgenic organism? a. an organism that has had its entire genome sequenced b. an organism that has genes from another species c. an organism that does not use DNA to specify proteins d. an organism with multiple copies of the same gene e. an organism that has been DNA profiled
Which of these conditions are always true of populations evolving due to natural selection? Condition 1: The population must vary in traits that are heritable. Condition 2: Some heritable traits must increase reproductive success. Condition 3: Individuals pass on most traits that they acquire during their lifetime. Select one: O a. Conditions 2 only O b. Conditions 1 and 2 Conditions 2 and 3 Conditions 1 only O c. O d.
1) One of the first studies extracted all the DNA from a biofilm in an acid mine drainage in northern California in 2004. Then cloned the DNA in E. coli, sequenced all of the cloned fragments and then attempted to reconstruct the genomes of the different individuals in the population. This approach is an example of what? -Transcriptomics -Genomics -Metatranscriptomics -Metagenomics -Proteomics
Which of the following is true about DNA manipulation? Select the best answer. A. all of these B. DNA has never been transferred between different organisms. C. Transferring DNA from one organism into another always kills the host organism. D. Whole genomes have been manipulated by humans for centuries; now individual genes ca be inserted into different organisms using a variety of techniques. E. DNA can't be manipulated.