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- Under what conditions are media typically sterilized in an autoclave?Explain about different types of bioreactors and how they are used in cell suspension culture. What are the advantage and limitations of each bioreactors?What are the main types of immobilization techniques used in biosensors? (with a deep explanation)
- Briefly explain that what is the important to use immobilization techniques in biosensor?..(explain under5 points)A microbiologist has isolated a new strain of bacteria, and is trying to characterize aspects of its growth. Three flasks of the same media have different amounts of NaCl added. Other growth conditions are identical, and the flasks inoculated at the same time. At regular intervals over a 3 hour period, culture samples are taken and turbidity is measured. Here are the absorbance values over time in a table and graphed. Absorbance (OD600) Time (min) 20% NaCl 3% NaCl 0% NaCl 0 0.05 0.05 0.05 30 0.06 0.05 0.05 60 0.075 0.06 0.05 90 0.11 0.07 0.049 120 0.25 0.1 0.048 150 0.5 0.105 0.055 180 0.7 0.11 0.055 210 0.99 0.15 0.06 240 1.22 0.18 0.055 270 1.35 0.19 0.06 300 1.5 0.2 0.06 330 1.6 0.25 0.062 360 1.7 0.3 0.06 Based on these data, which of the following can be determined about this bacterium? It is psychrophilic. It…In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and pressure at 15 psi for 15 to 20 min? What will happen if this was done in only for 10 min? Can you autoclave lab materials and decontaminate cultures at the same time?