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- Mannitol, a carbohydrate, is supplied as a 25% (w/v) solution. This hypertonic solution is given to patients who have sustained a head injury with associated brain swelling. (a) What volume should be given to provide a dose of 70. g? (b) How does the hypertonic mannitol benefi t brain swelling?After blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?An amino acid mixture consisting of phenylalanine, glycine, and glutamic acid is to be separated by HPLC. The stationary phase is aqueous and the mobile phase is a solvent less polar than water. Which of these amino acids will move the fastest? Which one will move the slowest?
- You have determined that a cell suspension contains 1 x 103 cells/mL. That cell suspension was prepared by adding 1 ml of a more concentrated cell suspension to 999 mL of phosphate buffered saline. What is the concentration of cells in the original cell suspension?How can contaminants in DNA and RNA samples affect absorbance at 260 and concentration results?Isotonic, hypotonic, and hypertonic IV (intravenous) fluid solutions are widely used in the healthcare setting. As a possible health care professional, you must know how each of the solutions work on the body and why they are given. a) When does each type of solution must be applied to a patient? b) What does each type of solution do the cells? Isotonic IV fluid solution Hypotonic IV fluid solution Hypertonic IV fluid solution|
- Both of the regular intravenous solutions administeredin medicine, normal saline and lactated Ringer’ssolution, are isotonic. Why is this important?What is correct?Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.
- can you label the drawing? Where are the 1 eicosapentanoic acid, 1 myristic acid and 1 oleic acid residues.A) Consider membrane filtration. What is concentration polarization and why does it occur? B) What are the important parameters that affect the diffusivity in/across membranes and what do The they mean physically? C) Consider the Krogh Cylinder model. What is the physical meaning of the critical radiusand why does it occur?Is the 25% sucrose solution (or syrup) a hypertonic, hypotonic, or isotonic solution?