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We have used SAR as an estimate of purity. How else could you test for the purity of the phycocyanin sample?
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- How else could you test for purity of the phycocyanin sample? Briefly outline the steps to be takenWhy is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.Select all of the following that represent examples of positive stains. A) nigrosin B) methylene blue C) crystal violet D) India ink E) safranin
- What is the relevance of the use of sugar, spice, and starch in the detection of sporeformers? What is the selective agent used in the detection of sporeformers from dried ingredients? Why is ropiness usually observed at the center of the bread?What is the use of xylol in staining milk preparation?You have to prepare an agarose solution for gel electrophoresis. How many grams of agarose powder do you need in order for you to make 50mL of 1% agarose solution?
- Different stains have different affinities for different organisms or different parts of organisms. Elaborate on this statement using the Hematoxylin and Eosin staining as an example.Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.List other fluorescent stains. What is the usefulness of each?