Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL
Q: E. coli ribonuclease H1 is an enzyme that catalyzes the hydrolysis of phosphodiester bonds in RNA. I...
A: Carboxylate relay One of the carboxylates ( Asp70) activates water molecule to release the hydroxyl ...
Q: What name is given to the assembly of proteins that carry out DNA copying at a replication fork? O c...
A: Every time cells divide, eukaryotic genomes are duplicated with near-perfect fidelity. The circumsta...
Q: Using Molisch's reagent, an unknown carbohydrate solution was found to be positive (+). Within three...
A: Molisch's test is done for detection of carbohydrate. Barfoed's test is used for detection of monosa...
Q: Why do we compute for TFM in soaps. How does one calculate for TFM? What is saponification? Show it...
A: 1. The TFM quantity interprets the quality of soap. It adds moisturizing properties to the product, ...
Q: 1. What is the name we give to structures which are not complete polypeptide chains but form superse...
A: "Since you have asked multiple questions, we will solve the first four questions for you. If you wan...
Q: structure of cysteine at pH 8.2 and what is its charge?
A: Cysteine is a sulfur-containing amino acid. It is an amino acid with an uncharged polar side chain a...
Q: 30. Sketch the O2-consumption curves obtained for the mitochondria in Problem 29 when (a) CN¯ is add...
A: The electron transport chain is the metabolic process that consumes oxygen and reduces it to water. ...
Q: Describe the types of pathogens. Give an example of an obligate pathogen, a facultative saprophyte, ...
A: Microorganisms, often known as microbes, are microscopic organisms that can be found all over the pl...
Q: Match the function with the appropriate protein or sequence:
A: tRNA is a short nucleotide chain with L-shaped structure. A tRNA molecule is required for translatin...
Q: 1. Would you say that indicator tests are qualitative or quantitative? Explain your answer.
A: “Since you have asked multiple questions, we will solve the first question for you. If you want any ...
Q: How does one measure Km using a Lineweaver-Burk plot? 1. By determining the reciprocal of the y-int...
A: Lineweaver-Burk plot is also known as double reciprocal plot suggested by Hans Lineweaver and Dean B...
Q: Imagine that you are viewing a slide with a heat-fixed smear from an sputum sample that has been aci...
A: Hi! Thank you for the question as per the honour code, we’ll answer the first question since the exa...
Q: In respiratory chain reaction, ATP is synthesis by. . O Coenzyme motive force AMP
A: The energy inside the cell is generated through catabolic processes such as glycolysis and the TCA c...
Q: 1. In one sentence describe the general structure and functions of the following biomolecules in a l...
A: Biomolecules or Biological molecules are the molecules present in living organisms and are essential...
Q: oxidation of NADH by nitrate produces 5 ATP, by H2S is 2 ATP, by O2 is 3 ATP. Why oxidation of NADH ...
A: An intact Krebs cycle is frequent in microbes that use anaerobic respiration, allowing them to utili...
Q: What is the impact of biochemistry of fats consumption especially having too much saturated fats in ...
A: Our body needs energy for the proper functioning of all the organs and to sustain the metabolic acti...
Q: 6. Delineate the steps in the production by a human of a C20:3 45, 8, 11 fatty acid. Make sure your ...
A: Fatty acid metabolism includes Fatty acid biosynthesis (an anabolic process) and β- oxidation o...
Q: 10 samples of genetically modified organisms ( Animals ) With brief descriptions of each
A: Genetically modified organisms (GMOs) are those organisms (plants, animals or microorganisms) whose ...
Q: 1. What organelles/parts of the cell are present in all types of cells, whether prokaryotes or eukar...
A: All living things are made up of cells, which are the structural, functional, and biological compone...
Q: do archaebacteria have autotroph or heterotroph nutrients
A: Nutrients are the components of food like carbohydrates, fats, vitamins, proteins, etc. These nutrie...
Q: Which of the following is NOT a dissimilarity between RNA and DNA? a) Types of bases b) Double-str...
A: RNA , DNA : Nucleic acids. RNA : Ribonucleic acid DNA : Deoxyribonucleic acid Both are structurall...
Q: How many phosphate groups are part of the nucleotide? (A) 2 only B) 1, 2, or 3 c) 1 only D) 3 only
A: S.No. DNA RNA 1 Deoxyribonucleic Acid. Ribonucleic Acid. 2 Generally double stra...
Q: 1. What are the ground electronic configurations of Ti and Ti* ion? Ti Tit
A:
Q: write the orbital box diagram and dipole moment using the lewis strcuture for clo3^-: orbital name, ...
A: Lewis structure: Lewis figures show only an atom's valence electrons and the chemical symbol of a c...
Q: Which isotope did Hershey and Chase use to label bacteriophage nucleic acid? 32p 355
A: A. D. Hershey and Martha Chase, two geneticists, offered more proof in 1952. They experimented with ...
Q: Is the phosphate functional group negatively charged in both a non-cellular and cellular environment...
A: A phosphate group is an important group that helps in the formation and hydrolysis of ATP molecules....
Q: When a protein denatures in a cell, it tends to assemble into aggregates. Propose an explanation for...
A: Proteins have distinct structures. If they are exposed to specific conditions, they may lose their s...
Q: Calculate volume for 10 ug of DNA.
A: DNA concentration is estimated by measuring the absorbance of a DNA sample in buffer solution at 260...
Q: Name the experiment shown above and briefly describe how it is set up as well as the role of each co...
A: Gel electrophoresis is a method of separation of protein (SDS-PAGE, polyacrylamide gel electrophores...
Q: You sequence a new tRNA and discover that the anticodon sequence is 5'-IGC-3. What amino acid does i...
A: I corresponds to an unusual bas hypoxanthine, which is found in tRNA
Q: What enzyme kinetic parameters are apparently impacted by competitive inhibitors? Vmax ...
A: Correct answer Option b. Km Explanation Competitive Inhibition: Competitive inhibition is a type...
Q: The type of bonding that happens between atoms within a water molecule is which of the following? a...
A: The molecules of water are held together by non-covalent interactions.
Q: What are the 4 levels of structural organization of proteins and what are the important interactions...
A: The four levels of structural organization of proteins are : Primary structure, Secondary structure,...
Q: Endosymbiotic theory can be applied to Biomolecules as it invloves the development of nucleic acid a...
A: Protein import is one of the strongest evidence of origin of organelles
Q: Draw a tripeptide only containing Alanine
A: Amino acids are the building blocks of proteins which are composed of amino group (NH3+), carboxyl g...
Q: is an example for aldotriose.
A: The question is about the functional group that out of given example which is the example of aldotri...
Q: a. What is the efficiency of the metabolic conversion of palmitic acid to ATP? b. Compute the numbe...
A: Saturated fatty acids include palmitic acid. It has a lengthy chain since the backbone is made up of...
Q: Abs undiluted 0.208 Diluted (1/10) 0.105 Diluted (1/20) 0.09 Use the chart above to back calculate...
A: Absorption spectroscopy is a spectroscopic method that measure the absorption of radiation by the sa...
Q: Which of the following molecules is an inorganic molecule? a) cholesterol b) sucrose c) collage...
A: Biomolecules are organic molecules made up of mainly carbon and hydrogen but there are other element...
Q: Is the picture below a D or L amino acid? Q2: Name 4 reducing sugars
A:
Q: ENDOSYMBIOTIC THEORY is the evolution theory that includes a larger and one smaller cell. The larger...
A: It is believed that life arrived on earth some four billion years ago and the first cells were proka...
Q: Formulate a developing heifer ration containing 14% CP on a DM basis. The feeds to be used are corn ...
A: Using pearson square method, the feed ration found out.
Q: Why is dietary fiber yood for you? What are some of its sources? What's the difference between solub...
A: Dietary fibers are carbohydrates. Fibers are mainly composed of polysaccharides. D...
Q: An athlete uses ATP (another chemical) as fuel for a sprint when a glucose molecule is broken down. ...
A: ATP is fundamental source of energy in humans and during sprinting we gets this ATP from different s...
Q: LUT3 iš homologous to human GLU 25. Rabbi threonine, two (2) serine and one (1) asparagine. Based on...
A: Since the environment within the lipid bilayer is nonpolar, the transmembrane regions of the the mem...
Q: Experiment No. 2 AMINO ACIDS AND PROTEINS Data Solubility in water Sample Solubility (So...
A: Disclaimer: "Since you have posted a question with multiple subparts, we will solve the first three ...
Q: 1a-Membrane bound proteins often contain transmembrane domains. These transmembrane domains cont...
A: Membrane proteins can be of three major types- membrane bound/integral, peripheral and lipid anchore...
Q: What are the different types of solutions according to their concentration? What is its effect of ea...
A: Solutions: A solution is a homogenous mixture that contains a similar kind of substance (solute) di...
Q: It's important to note that MnO is an alkaline metal, whereas Mn207 is an acidic metal. Why? (ii) Al...
A: Introduction Chemical compounds are a critical component of life. Organic substances are those deriv...
Q: Protein folding
A: proteins are composed of some 20 types of amino acid residues, and they would be more or less alike ...
Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μL
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.How is 10^4 derived? Please show all calculation steps. # of cells per mL = (Average)*DF*10^4
- You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.An IV bag contains 250 mL of a 5% mannitol solution. How many grams of mannitol are in the IV bag?How much serum is needed to create 2μL of a 1:20 dilution?
- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable number of cells in original culture (CFU/ml)?You have a 1:5 dilution. Describe how you would make a 1:100 dilution. When 0.5 µL of serum is added to 1.0 µL of diluent, what is the dilution? What is the dilution factor needed to change a 1:2 dilution to a 1:10?