The nuclear chromosomes in all of the cells of the clone are derived only from the somatic nuclear donor, the cloned animal and this donor are not perfectly identical in all respects, for which reasons?
Q: Describe the process of somatic cell nuclear transfer and how it is used to create cloned cells.…
A: As per the guidelines, the first question is answered. Somatic nuclear cell transfer (SCNT) is a…
Q: It is possible to take the DNA of a gene from any source and place it on a chromosome in the nucleus…
A: Recombinant DNA technology is the process in which the recombination of genetic material takes place…
Q: Transposons are useful as mutagens because they act asmolecular tags for genomic DNA sequences that…
A: Step 1 Transposons or jumping gene is a DNA (deoxyribonucleic acid) sequence that can change its…
Q: Which of the following is NOT true of transposition? O A) a replicative form of transposition is…
A: Transposable elements are the jumping genes.
Q: If telomeres are normally shortened after each round of replication in somatic cells (see Chapter…
A: Cloning is the creation of identical biological entities for therapeutic and reproduction. It is…
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Q: In reverse transcriptase polymerase chain reaction (RT-PCR), an analytical method used to amplify…
A: Reverse transcriptase polymerase chain reaction (RT-PCR) is a method used to amplify and sequence…
Q: In yeast, you have sequenced a piece of wild-type DNA and it clearly contains a gene, but you do not…
A: To find the mutated genes one must code all the amino acid of the genetic modified yeast and normal…
Q: Do a few cells created by therapeutic cloning of your own somatic cells constitute life? If these…
A: Somatic cells are those cell which forms the whole body of an organisms, also known as the vegetal…
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A: Restriction enzymes cut deoxyribonucleic acid molecules at specific sites. Restriction endonucleases…
Q: Describe the process of somatic cell nuclear transfer and how it is used to create cloned cells.…
A: Somatic cell nuclear transfer is also called therapeutic cloning. Here the somatic cell's nucleus is…
Q: The human genome contains about 3 billion base pairs. During the first cell division after…
A: In the S-phase of interphase, DNA replication occurs in both mitosis and meiosis. DNA replication…
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A: Yeast has a very compact genome having 70 % of its DNA as exons, therefore it contains insufficient…
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A: A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from…
Q: In eukaryotes, sexual reproduction is the main process by which new gene combinations are generated.…
A: Genetic material passes on from parents to offspring along with the information loaded in the form…
Q: Although a number of different animals have been successfully cloned, the process of creating cloned…
A: A living organism, which is genetically identical to that of the other organism is known as a Clone.…
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A: Cancer is a disease that is associated with uncontrolled division of cells and invasion of…
Q: Which of the following contain only the sequences required for transposition and the genes for…
A: Transposons are the DNA sequence which can change their position within the genome. It may be simple…
Q: What are some of the reasons you might expect of human cells to have more efficient repair systems…
A: DNA (deoxyribonucleic acid) repair refers to the set of processes that are involved in the…
Q: A line of mouse cells is grown for many generations in a medium with 15N. Cells in G1 are then…
A: A mouse cell that has been growing in a 15N -rich medium for generations will have only the 15N…
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A: Hi, Thanks For Your Question. Ans : Cancer Cells Telomeres are sequences of DNA which are short and…
Q: Which of the following mobile genetic elements is a retrotransposon, making up about 10% of the…
A: A transposon can be defined as the characteristic mobile genetic element. These elements can…
Q: Explain why can a clone can be produced from a single body cell adult?
A: Clones are organisms shaped by the asexual technique of replica. it's the assembly of a replacement…
Q: import processes of histones which are synthesized in the cytosol but function in the nucleus to…
A: Replication and Transcription Replication is a process in which DNA makes copies of itself to ensure…
Q: The following diagram outlines how the process of cloning a sheep was accomplished. Cloning is the…
A: gene cloning is a process of producing the exact same of the gene of the interest. Replicating…
Q: . In yeast, you have sequenced a piece of wild-type DNAand it clearly contains a gene, but you do…
A: Introduction In the RDT (recombinant DNA technology), we insert the segment of DNA into the vector…
Q: For each of the following scenarios, indicate Yes (it is cloning) or NO (it is NOT cloning)…
A: Cloning means an organism is generated genetically identical from an existing organism through…
Q: involved in the transfer of multiple drug resistance from one cell to another? * a.Transposition b.…
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Q: A major difference between prokaryotes and eukaryotes is the presence of a nucleus. What advantages…
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Q: Satellite DNA Select one: Is found outside of the nucleus Is a term used to describe very small…
A: Satellite DNA consists of large amount of tandem repeats that are non coding (do not produce any…
Q: What would be the likely result of expressing telomerase in somatic cells? Immortality of gametes…
A: Telomerase is also known as telomere terminal transferase enzyme which is formed of RNA subunits and…
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Q: List all the materials needed to clone a mouse. Place the following steps in the correct order:…
A: Cloning is defined as a process of generating a genetically identical organisms either naturally or…
Q: during anaphase of animal cells, describe the appearance of the DNA, spindle fibers and location of…
A: Anaphase is the intermittent stage between metaphase and telophase where segregation of chromosomes…
Q: Select the following statements that DO apply to the process of non-homologous end joining. It can…
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Q: Does reproductive cloning always involve somatic cell nuclear transfer?
A: Gene cloning refers to the cloning fo genes through various techniques. These copies of genes can be…
Q: Select the following statements that DO apply to the It can be error prone. A. B. It uses a sister…
A: Repairing genetic material is a crucial phenomenon that aids in the proper inheritance of…
Q: DNA polymerase III can synthesize new DNA at a rate of 1000 bases per second. The genome of…
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Q: The genome of D. melanogaster consists of approximately 1.7 * 108 base pairs. DNA synthesis occurs…
A: Since it is a bidirectional replication, Genome of D.melanogaster= 1.7*108 bp Rate of DNA synthesis=…
The nuclear chromosomes in all of the cells of the clone are derived only from the somatic nuclear donor, the cloned animal and this donor are not perfectly identical in all respects, for which reasons?
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- A major difference between prokaryotes and eukaryotes is the presence of a nucleus. What advantages and disadvantages may occur with having a cell’s genome packaged in a nucleus?The technique of fluorescence in situ hybridization (FISH) is described. This is another method for examining sequence complexity within a genome. In this method, a DNA sequence, such as a particular gene sequence, can be detected within an intact chromosome by using a DNA probe that is complementary to the sequence.For example, let’s consider the β-globin gene, which isfound on human chromosome 11. A probe complementary to theβ-globin gene binds to that gene and shows up as a brightly colored spot on human chromosome 11. In this way, researchers can detectwhere the β-globin gene is located within a set of chromosomes. Becausethe β-globin gene is unique and because human cells are diploid(i.e., have two copies of each chromosome), a FISH experimentshows two bright spots per cell; the probe binds to each copy ofchromosome 11. What would you expect to see if you used thefollowing types of probes?A. A probe complementary to the Alu sequenceB. A probe complementary to a tandem array near…Describe the process of somatic cell nuclear transfer and how it is used to create cloned cells. Explain what you consider to be the positive and negative aspects of cloning both scientifically and morally, giving at least three 'for' and three 'against' points
- It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?It is possible to take the DNA of a gene from any source and place it on a chromosome in the nucleus of a yeast cell. When you take DNA of a human gene and put it into a yeast cell chromosome, the yeast cell can synthesize the human protein. However, when you remove the DNA for a gene normally present on yeast mitochondrial chromosomes and put it on a yeast chromosome in the nucleus, the yeast cell cannot synthesize the correct protein, even though the gene comes from the same organism. Explain. What would you need to do to ensure that such a yeast cell could make the correct protein?What is the difference between the cloned animal and nuclear donor in terms of X chromosome inactivation?
- Cell cultures can be synchronized, or brought into the same phase of the cell cycle, by various means. For example, adding thymidine to a cell culture causes all cells to become arrested early in S phase. What is the mechanism by which thymidine treatment blocks DNA replication?The genome of D. melanogaster consists of approximately 1.7 * 108 base pairs. DNA synthesis occurs at a rate of 30 base pairs per second. In the early embryo, the entire genome is replicated in five minutes. How many bidirectional origins of synthesis are required to accomplish this feat?You have a strain of Neurospora that is unable to synthesize histidine and thus requires H in the media in order to grow. You have isolated one revertant colony. Predict the expected proportion of the progeny that would be h+ if you cross the colony with the original mutant colony and the reversion occurred by each of the following mechanisms: Precise change of the mutated base back to its original base. A suppressor gene is mutated on a different chromosome A suppressor gene is mutated on the same chromosome but 10mu distant from the mutated gene. The mutant colony is crossed to a wild-type Neurospora colony and the following data are collected. 95% of all asci scored are h+ but 5% are h-. Which mechanism in part a is consistent with these data? Explain why and what has happened on a molecular level.
- For the following situations, indicate whether homologous or non-homologous DNA strands are preferred to use as template: Prokaryotic double strand break repair: Mitotic double strand break repair: Meiotic double strand break repair:Why is the technique used to clone mammals called somatic cellnuclear transfer?Do all of them please Multiple choice 1) Although Dolly was only three years old, her chromosomes had the length of a (9-10)-year old sheep. Which of the following best describes why this occurred?A) The cell lines that created Dolly were aged prematurely in the labB) Nonhomologous recombinationC) A mutation enhanced the rate of agingD) Dolly was not a cloneE) The telomeres of the somatic cells that Dolly originated from were shorteningAnswer: 2) Which of the following terms represents a cell that can form any other cell of the organism?A) PluripotentB) TotipotentC) UnipotentAnswer: 3) Bone marrow transplants typically use what type of cells?A) Embryonic stem cellsB) Embryonic germ cellsC) Embryonic carcinoma cellsD) Hematopoietic stem cellsAnswer: 4) Which of the following is an example of a pluripotent cell?A) Embryonic stem cellsB) Red blood cellsC) Fetal heart cellsD) Umbilical cord bloodE) Nerve cellsAnswer: 5) An organism that has integrated recombinant DNA into its genome is…